脑脉舒康胶囊对大鼠血瘀合并脑缺血模型能量代谢的影响

目的:探讨脑脉舒康胶囊对大鼠血瘀合并脑缺血模型能量代谢的影响.方法:采用注射地塞米松复制血瘀模型,模型大鼠灌服脑脉舒康胶囊0.9,0.6,0.3 g·kg-1连续10d,于第11天给药后1h,结扎大鼠两侧颈总动脉30 min,制造脑缺血模型;取大鼠脑组织匀浆,检测脑匀浆液Na+K+-ATPase,Mg2+ -ATPase,Ca2+-ATPase活力、乳酸(LD)含量、乳酸脱氢酶(LDH)活力.结果:脑脉舒康胶囊3个剂量组均可以提高模型大鼠脑组织ATP活力(P＜0.01或P＜0.05),高剂量和中剂量组可以降低LD含量(P＜0.01),中剂量和低剂量组可以提高LDH活力(P＜0.01).结论:脑脉舒康胶囊对大鼠血瘀合并脑缺血模型能量代谢有改善作用,减少乳酸堆积,可以减轻组织结构及功能损伤.

Effects of Naomaishukang Capsule on Rat Stasis Model Combined Cerebral Ischemia in the Energy Metabolism

Objective: To study the effects of Naomai Shukang capsule on energy metabolism in rat blood stasis with cerebral ischemia models. Method: Blood stasis model was replicated with injection of dexamethasone. Model rats were administrated orally with Naomai Shukang capsule according to dosages of 0.9,0.6,0.3 g·kg^-1 for 10 d. After the rats were administrated with drugs for 1 h on the 11th day, Cerebral ischemia models were made by ligating both sides of the common carotid arteries of rats for 30 min. Na⁺-K⁺-ATPase, Mg²⁺-ATPase, Ca ²⁺-ATPase activity, lactic acid(LD) content and lactate dehy drogenase(LDH) activity of brain homogenates were measured. Result: Three doses of Naomai Shukang capsule can improve the ATP activities(P<0.01 or P<0.05). The high and medium dose can reduce the LD content(P<0.01). The medium and low dose can increase the LDH activity(P<0.01). Conclusion: Naomai Shukang capsule can improve brain energy metabolism in rat blood stasis with cerebral ischemia models and reduce lactic acid accumulation and damage to brain tissue.