芪泽汤对慢性肾功能衰竭大鼠 内质网应激相关凋亡分子表达的影响

目的:探讨芪泽汤对慢性肾功能衰竭(CRF)大鼠内质网应激(ERS)相关凋亡分子表达的影响.方法:2.5％腺嘌呤ig24 d建立SD大鼠肾衰模型.分为5组:正常组、模型组、尿毒清组(2.5 g·kg-1·d-1)、芪泽汤高、低剂量组(50.4,12.6g·kg-1·d-1),每组10只.造模同时各治疗组ig给药,干预24 d后,观察体重变化,全自动生化分析仪检测大鼠血尿素氮(BUN)、血肌酐(SCr),应用TUNEL法检测肾组织细胞凋亡,Western blot检测CHOP( C/EBP-homologous protein)和半胱氨酸天冬氨酸蛋白酶12( Caspase-12)肾内表达水平.结果:模型组BUN(17.21±4.63) mmol·L-1,SCr( 67.00±25.62) μmol·L-1较对照组BUN(4.19±0.77) mmol· L-1,SCr( 15.89±2.62)μmol·L-1明显升高(P ＜0.01)；TUNEL结果显示空白组肾小管上皮细胞有少量凋亡,而模型组细胞凋亡数明显增多(P＜0.01)；模型组CHOP,Caspase-12表达显著增强(P＜0.01).芪泽汤低剂量和尿毒清能抑制慢性肾功能衰竭大鼠肾小管上皮细胞凋亡和CHOP,Caspase-12表达(与模型组相比较P＜0.05).结论:芪泽汤低剂量能保护腺嘌呤导致的大鼠肾损害,可能与其抑制ERS相关凋亡分子CHOP,Caspase-12高表达有关.

Effects of Qize Decoction on the Expression of Endoplasmic Reticulum Stress Related Apoptosis Molecules in Chronic Renal Failure Rats

Objective:To study the effect of Qize decoction at endoplasmic reticulum stress(ERS) in rats with chronic renal failure(CRF).Method: SD rats were fed with adenine of 2.5% to establish chronic renal failure model.They were divided into five groups:normal group,model group,Niaoduqing group(2.5 g·kg-1·d-1),Qize decoction of high dosage(50.4 g·kg-1·d-1)group and Qize decoction of low dosage(12.6 g·kg-1·d-1) group,each group has 10 rats.All groups were infused with adenine into stomach in addition to normal group,at the same time,each rat in chinese herbal compound group was also infused with chinese herbal compound into stomach and Niaoduqing group was given Niaoduqing liquid for 24 days.Body weight was observed dynamically,blood urea nitrogen(BUN) and serum creatinine(SCr) levels were checked by automatic biochemical analyzer.The apoptosis of renal was evaluated by means of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL).The expression of C/EBP-homologous protein(CHOP) and Caspase-12 was detected by Western bloting.Result: Compared with the control group BUN(4.19±0.77)mmol·L-1 and SCr(15.89±2.62)μmol·L-1,in the model group BUN(17.21 ±4.63) mmol·L-1 and SCr levels(67.00 ±25.62) μmol·L-1 were much higher(P<0.01).The results of TUNEL showed that the few numbers of apoptosis in renal tubular epithelial cells in normal group were found,but more in model group.The expression of CHOP and Caspase-12(P<0.01)were much higher in the model group.Compared with the model group,the number of apoptosis in renal tubular epithelial cells and the expression of CHOP and Caspase-12 were reduced(P<0.05) in Qize decoction low dosage group and Niaoduqing group.Conclusion: Qize decoction low dosage could protect the adenine-induced renal damage,maybe related to that it can inhibit the high expression of ERS-related apoptosis-molecule CHOP and Caspase-12.