不育男性精浆酸性磷酸酶和锌与精液参数分析

目的:探讨不育男性精浆中酸性磷酸酶和锌水平与精子密度及精子活力的关系。方法:对21例正常生育男性、169例不育男性精浆中酸性磷酸酶和锌分别进行检测,并作白细胞染色计数。结果:①不论按精子密度分组或按精子活力分组,不育各组精浆中酸性磷酸酶含量明显低于正常对照组(P<0.01),但不育各组之间的差异无显著性意义(P>0.05);②不论按精子密度分组或按精子活力分组,不育各组精浆锌含量仅死精子症组低于正常对照组(P<0.05);③按精子密度分组,各组标本之间白细胞计数的差异用秩和检验分析,不育各组白细胞计数的平均秩次明显大于正常对照组(P<0.001);按精子活力分组,不育各组精浆白细胞计数均高于正常对照组(P<0.05),死精子症组除外(P>0.05)。④精浆中酸性磷酸酶及锌含量均与白细胞计数不相关(r=0.088,P=0.162;r=0.119,P=0.057)。结论:精浆中酸性磷酸酶水平下降和白细胞增多均可导致精子密度和精子活力降低,可作为男性不育的诊断指标;不育患者精浆中锌离子水平虽然低于正常对照组,但无显著性差异。

Analyse of Zinc and Acid Phosphatase in Seminal Plasma and Sperm Parameters of Infertile Male

Objective: This study was to investigate the relationship among zinc,acid phosphatase levels in seminal plasma and sperm parameters in infertile male.Methods: The activities of zinc and acid phosphatase in seminal plasma were detected and leucocytes was stained and counted in 169 infertile men and 21 normal fertility as control.Results: Acid phosphatase levels in infertile groups were significantly lower than those in the normal control when grouped both in terms of sperm vitality and density(P<(0.01)),but no statistical differences were observed among infertile groups(P>(0.05)).Further,zinc levels in necrospermia groups were lower than those in the control(P>(0.05)).The leucocyte numbers in infertile groups were larger than those in the control when grouped according to sperm density(P<(0.001)),and the same results were obtained when grouped in terms of pserm vitality except in the necrospermia group(P>(0.05)).Acid phosphatase and zinc levels were not correlated with leucocyte counting(r=(0.088),P=(0.162);r=(0.119),P=(0.057)).Conclusion: The descent of acid phosphatase and ascent of leucocyte number in seminal plasma can result in the fall of sperm viatality and density,and can be used to be diagnostic indexes of male infertility.Serminal zinc levels in infertile groups were lower than those in the control,but there was no statistical significance.