| 地黄脱毒技术研究 |
| |
| 引用本文： | 武宗信,解红娥,冯文龙,解晓红,陈丽,李红霞,李江辉. 地黄脱毒技术研究[J]. 中药材, 2002, 25(6): 383-385 |
| |
| 作者姓名： | 武宗信 解红娥 冯文龙 解晓红 陈丽 李红霞 李江辉 |
| |
| 作者单位： | 山西省农科院棉花研究所,运城,044000 |
| |
| 摘 要： | 从地黄种茎育出的无菌幼苗上切取下的茎尖,用组织培养方法可诱导成去病毒幼苗。本试验还研究出适宜地黄茎段苗消毒的消毒剂为0.05％HgCl_2。适宜于茎段苗生长的MS培养基的琼脂浓度为0.7％,pH为7。诱导茎尖成苗的培养基为MS 6-BA 0.5 mg/L。繁殖脱毒苗所用的MS培养基为1/4大量元素 1/2微量元素,并用1/2食用白砂糖代替蔗糖,可使快繁成本降低48.7％。
|
| 关 键 词： | 地黄Rehmannia glutinosa 脱毒 茎尖 诱导 |
| 修稿时间： | 2001-12-14 |
Study on the Technique of Rehmannia glutinosa Virus-free |
| |
| Wu Zongxin,Xie Honge,Feng Wenlong,Chen Li,Li Hongxia,Li Jianghui. Study on the Technique of Rehmannia glutinosa Virus-free[J]. Journal of Chinese medicinal materials, 2002, 25(6): 383-385 |
| |
| Authors: | Wu Zongxin Xie Honge Feng Wenlong Chen Li Li Hongxia Li Jianghui |
| |
| Affiliation: | Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000. |
| |
| Abstract: | The stem tip from germ-free stem segment of Rehmannia glutinosa cultured in test tube can be induced into virus-free seedling.The experiment showed that the proper disinfectant for stem segments of Rehmannia glutinosa was 0.05% HgCl2.The seedings from stem segments grew better with MS in the concentration of agar 0.7% and pH7. The stem tips could be directly induced to seedlings by using MS 6-BA(0.05 mg/L) .The MS media for seedlings virus-free culture are 1/4 macro-elements 1/2 micro-elements and using edible sugar instead of sucrose,so the cost of media could be decreased to 48.7%. |
| |
| Keywords: | Rehmannia glutinosa Virus-free Stem tip Inducement |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
