P21ras、VEGF、Survivin在胃癌组织中的表达及意义

目的：探讨P21ras基因、血管内皮生长因子（VEGF）、凋亡抑制因子Survivin在胃癌组织中的表达及其与胃癌生物学行为的关系。方法：应用免疫组化（SP）法检测P21ras、VEGF;应用原位杂交法检测Survivin mRNA在12例正常胃粘膜组织、23例胃良性疾病组织及57例胃癌组织中的表达。结果：P21ras基因在正常胃粘膜组织、胃良性疾病组织及胃癌组织中的表达阳性率分别为16．7％（2／12）、21．7％（5／23）和56．1％（32／57）；VEGF表达阳性率分别为0、8．7％（2／23）和68．4％（39／57）；Survivin mRNA表达阳性率分别为0、0和54．4％（31／57）．胃癌组与正常粘膜及胃良性疾病组之间的差异有统计学意义（P〈0．01）．而正常胃粘膜与胃良性疾病组之间的差异无统计学意义（P〉0．05）。P21ras、VEGF的表达与胃癌侵袭深度、淋巴结转移及肿瘤临床分期相关（P〈0．05），而与年龄、性别、组织学分化程度无关（P〉0．05）；Survivin mRNA的表达与胃癌组织学分化程度、侵袭深度、淋巴结转移及临床分期相关（P〈0．05），而与年龄、性别无关（P〉0．05）。VEGF在胃癌组织中的表达与P21ras和Survivin mRNA呈正相关（P〈0．05）。结论：胃癌组织中P21ras、VEGF、Survivin mRNA的表达与肿瘤侵袭深度、淋巴结转移及临床分期密切相关，且VEGF与P21ras、Survivin mRNA的表达呈正相关，联合检测P2lras、VEGF、Survivin有助于提高胃癌侵袭转移能力的评估．为判断胃癌患者的预后提供参考。

Expression and significance of P21ras, VEGF and survivin in gastric cancer tissues

Objective: To identify the expression of P21ras gene, vascular endothelial growth factor(VEGF) and Survivin in gastric carcinoma and its relationship to the biological behavior of gastric carcinoma.Method: The expression of P21ras and VEGF were detected by immunohistochemistry SP method and the expression of Survivin mRNA was examined by in situ hybridization technique in 57 malignant,23 benigns and 12 normal gastric tissues.Result: The positive rates of P21ras gene in malignant,benigns and normal gastric tissues were 16.7%(2/12),21.7%(5/23) and 56.1%(32/57);The positive rates of VEGF in malignant,benigns and normal gastric tissues were 0%,8.7%(2/23) and 68.4%(39/57);and the positive rates of Survivin mRNA were 0%,0% and 54.4%(31/57).The positive rates in malignant gastric lesions were significantly higher than that of the other two groups(P<0.01).The difference was not statistically sigificant between benign and normal gastric tissues(P>0.05).The expression of P21ras,VEGF were positively correlated with the depth of tumor infiltration,lymph node metastasis and clinic stage(P<(0.05)),but was not related to age,sex and the degree of histological differentiation of the patient(P>(0.05)).The expression of Survivin mRNA was positively correlated with the the degree of histological differentiation,depth of tumor infiltration,lymph node metastasis and clinical stage(P<0.05),but was not related to age,sex of the patient(P>0.05).The expression of VEGF in gastric carcinoma was positively correlated with P21ras and Survivin mRNA(P<0.05).Conclution: The expression of P21ras,VEGF and Survivin mRNA were significantly correlated with depth of infiltration,lymph node metastasis and clinical stage of gastric carcinoma.The expression of VEGF in gastric carcinoma was positively correlated with P21ras and Survivin mRNA.The combined detection of P21ras,VEGF and Survivin may be the valuable(indicators) to evaluate the infiltrative ability and prognosis of gastric carcinoma.