改良的Tricine-SDS-PAGE电泳检测胸腺肽分子量

目的 建立一种分析胸腺肽制剂中多肽分子量分布的简便可行的方法。方法 采用改良的Tricine-SDS-PAGE电泳方法分析胸腺肽制剂中多肽的分子量分布,并分析其中胸腺素α1的含量。结果 采用该法可检出1μg的胸腺肽α1,分析国内市场上的胸腺肽制剂多肽分子量分布范围在3.5～8.5kD。结论 该法适用于分析胸腺肽制剂中多肽组分的分子量分布和胸腺肽α1的存在,可用于胸腺肽制剂的质量控制。

Analysis of the Molecular Weight of Peptides in Thymosin by Tricine-SDS-PAGE

Objective To establish a simple and feasible method in analyzing the molecular weight distribution of peptides in thymosin.Methods Improved Tricine-SDS-PAGE electrophoresis was applied to detect the molecular weight of peptides and the content of thymosin α1 in thymosin.Results Tested the thymosin preparations on the domestic market using this improved method.It was demonstrated that the peptide molecular weight distribution in thymosin preparations was between 3.5～8.5 kD,also could detect the concentration of 1 μg thymosin α1 in thymosin by using this improved method.Conclusion This improved method is suitable for the analysis of peptides molecular weight distribution and theconcentration of thymosin α1,so it can be used for control quality of thymosin preparations.