红霉素体外诱导金黄色葡萄球菌耐药分析

目的 体外诱导金黄色葡萄球菌标准株ATCC25923对红霉素耐药,分析其耐药后菌株生长及对其它药物敏感性变化。方法 用红霉素浓度2倍递增的方法对金黄色葡萄球菌ATCC25923进行体外诱导,比较诱导前后金黄色葡萄球菌的生长表型以及对其它抗菌药物的耐药性变化。结果 诱导后ATCC25923红霉素MIC由0.032 5 mg/L增加到>256 mg/L,诱导耐药后金黄色葡萄球菌出现生长缓慢,溶血性减弱等变异,对其它非诱导抗菌药物耐药性未发生变化。结论经红霉素体外诱导后金黄色葡萄球菌可以获得稳定耐药性,但同时也产生了表型及部分生化特性变化,与其它非诱导抗菌药物无交叉耐药。

Drug-Resistance of Staphylococcus Aureus Induced by Erythromycin in Vitro

Objective To investigate the drug-resistance of Staphylococcus aureus induced by erythromycin in vitro and its changes ingrowth and susceptibility of antibiotics.Methods Erythromycin in vitro induction was conducted with the S.aureus reference strainATCC25923 on the serial of erythromycin agar plates.The growth of S.aureus,and the susceptibility against other antibiotics was compared after induced tothe parent strain.Results Resistance to erythromycin was successfully,and themaximum MIC was over 256 mg/L.The Erythromycin-resistant S.aureus grew much slower than the susceptible parent,and the strains didn't have cross-resistance to other antibiotics.Conclusion S.aureus could be induced resistance in vitro by erythromycin,and this resistance inherited stably.Some phenotype and biochemical characteristic features of the strain were changed after induced.