蛋白质组学在胰腺疾病研究中的应用

蛋白质组学是研究蛋白质的表达、翻译后修饰、在细胞内定位以及蛋白质与蛋白质之间的相互作用的科学。传统的研究手段主要采用双向凝胶电泳和质谱技术,前者用于蛋白质的分离,后者用于蛋白质的鉴定。目前新兴的研究技术主要有相差凝胶电泳技术同位素亲和标签技术。蛋白质组学在胰腺疾病的研究主要是通过正常个体与患病个体的血清、胰液或组织等进行蛋白质比较,发现差异蛋白质,从而对急、慢性胰腺炎和胰腺癌等胰腺疾病的发病机制、早期诊断和有效治疗提供理论依据和新思路。

Proteomics in Pancreatic Disease

Proteomics is a subject that aims to study the expression, translation and location of proteins, the relationship between proteins and proteins as well. Two-dimensional gel electrophoresis and mass spectrometry are traditional methods, the former serves to separate proteins while the later to identify the different proteins. Some new techniques such as differential in-gel electrophoresis and Isotope coded affinity tag are more and more widely used these days. Plasma, pancreatic tissue and juice were taken from normal and sick individuals who were suffered from acute pancreatitis, chronic pancreatitis or pancreatic cancer. Comparing the different proteins between normal and sick individuals could be a useful way for early diagnosis and effective treatment for pancreatic disease.