沙眼衣原体感染对大鼠着床期间子宫内膜上皮细胞的影响

目的:研究沙眼衣原体(Ct)感染后大鼠着床期间子宫内膜形态学改变及肌动蛋白的变化。方法:大鼠雌雄合笼建立早孕模型,取正常妊娠组和Ct感染后妊娠组大鼠子宫,采用扫描电镜(SEM)观察子宫内膜超微结构改变及荧光免疫组化检测肌动蛋白的表达。结果:SEM示对照组子宫腔可见有微绒毛的分泌细胞;实验组腔上皮细胞表面有的可见破损,微绒毛少见;免疫组化结果显示荧光强度主要定位于腔上皮细胞胞质/胞膜,妊娠第4,5,6天沿胞质顶部分布较密集,而底部较弥散;妊娠第7天顶部强度有所增加;感染组的荧光强度均弱于相应正常组,在妊娠第5天存在显著性差异(P<0.05)。结论:沙眼衣原体感染后可能通过破坏子宫腔上皮分泌细胞,进而影响其内成分肌动蛋白,从而干扰早期妊娠。

Effects of Chlamydia Trachomatis on Endometrium Epithelial Cells During Rats Implantation Window

Objective: To study the changes of morphology and actin level in the endometrium of rats during implantation window after Chlamydia trachomatis(Ct) infection.Methods: The uteri of pregnancy rats with and without Ct infection were sectioned.Scanning electron microscopy(SEM) was used to study the ultra-structure of the uterine luminal epithelial cells and immunofluorescence method was used to study the expression of actin.Results: The normal epithelial cells were intactly lined with microvilli,and lesions on the surface of Ct infected cells were showed by SEM.The expression of actin was obvious throughout the cytoplasm of uterine epithelial cells but appeared to be most intense along the apical plasma membrane on day 4,5 and 6 of pregnancy.On the day 7,levels of actin had increased along the apical surface.The expression of actin in the infected group was weaker than that in of the normal group.And there was a significant difference on day 5 between the two groups.Conclusion: Ct infection could influence the early pregnancy through the epithelial cells.