| 组织工程新材料蜘蛛拖丝蛋白的重组培养 |
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| 引用本文： | 章文贤,李敏,涂桂云,黄智华.组织工程新材料蜘蛛拖丝蛋白的重组培养[J].中国组织工程研究与临床康复,2003,7(20):2782-2784. |
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| 作者姓名： | 章文贤 李敏 涂桂云 黄智华 |
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| 作者单位： | 福建师范大学生物工程学院生化与分子生物学系,福建省福州市,350007 |
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| 基金项目： | 国家教委资助项目(JA02158)，福建省自然科学基金重大项目(2001F006)~~ |
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| 摘 要： | 目的通过对重组蜘蛛拖丝蛋白基因工程菌pNS2生长的培养基进行优化,为获取大量蜘蛛拖丝蛋白提供条件。方法采用单因子试验和正交试验对培养基进行优化。结果获得优化的培养基组成:葡萄糖5.0g/L、蛋白胨5.0g/L、磷酸二氢钾(KH2PO4)15.0g/L、微量元素母液15.0g/L、硫酸镁(MgSO4)0.8g/L、柠檬酸1.7g/L。培养基优化后,工程菌的生长量提高了60.6%。结论优化的培养基为基因工程菌pNS2大规模生产蜘蛛拖丝蛋白奠定了基础。
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| 关 键 词： | 生物医学工程 生物相容性材料 培养基 正交试验 |
| 文章编号： | 1671-5926(2003)20-2782-03 |
| 修稿时间： | 2003年3月4日 |
Recombinant culture of spider dragline silk protein as a new tissue engineering material |
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| Wen-Xian Zhang,Min Li,Gui-Yun Tu,Zhi-Hua Huang,Coll ege of Bioengi-neering,Fujian Normal University,Fuzhou ,Fujian P rovince,Chi na.Recombinant culture of spider dragline silk protein as a new tissue engineering material[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2003,7(20):2782-2784. |
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| Authors: | Wen-Xian Zhang Min Li Gui-Yun Tu Zhi-Hua Huang Coll ege of Bioengi-neering Fujian Normal University Fuzhou Fujian P rovince Chi na |
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| Institution: | Wen-Xian Zhang,Min Li,Gui-Yun Tu,Zhi-Hua Huang,Coll ege of Bioengi-neering,Fujian Normal University,Fuzhou 350007,Fujian P rovince,Chi na |
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| Abstract: | Aim To optimize the medium for the growth of genetic e n -gineering Escherichia coli pNS2,pr oviding a nice condition for the mass cul ture of spider dragline silk proteins.Methods The single-factor experi-ments and the orthogonal experiment s were adopted to opitimize the medi-ums.Result s The optimized culture medium consis ted of 5.0g /L Glucose,5.0g /L Pe ptone,15.0g /L KH 2 PO 4 ,15.0g /L Trance elements storeso-lution,0.8g /L MgSO 4 ,1.7g /L Citric acid.The growth of en gineering bacteria increased 6 0.6%in the optimized culture.Conclusion The opti-mized culture medium is u sed for genetic engineering Escherichia coli pN S2to produce the recombinant sp ider dr agline silk protein in a large scale. |
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| Keywords: | biomedical engin eering biocompatible materials cul-ture media orthogonal test |
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