酶联免疫吸附试验(ELISA)和动物法测定人血清破伤风抗体的比较

<正> 人血清中破伤风抗体的测定,在临床上和流行病学上都有十分重要的意义。在临床上是判断病人对破伤风是否具有免疫能力的重要依据;在流行病学上是监测人群破伤风免疫状态的重要方法。小白鼠中和试验是检测人血清破伤风抗体水平的经典方法,凡是抗体效价达到0.01~(IU)/ml以上者便具有足够的破伤风免疫力,动物法的结果准确可靠,为

COMPARISON FOR DETECTION OF TETANUS ANTIBODIES BY USING ELISA AND ANIMAL TEST

This article aims at the comparison of the results obtained between the use of ELISA (using β-Galactosidase as label) and the animal method in anti-tetanus antibody serum levels.Human sera from 112 healthy individuals were tested for tetanus antibodies by using ELISA and Mouse neutralization test. The results for ELISA and animal test showed a good agreement. The positive sera detected by ELISA and animal test were 47 and 53 respectively, the corresponding rate of both method being 88.7%. And negative sera detected by ELISA and animal test were 57 and 59 respectively, the corresponding rate of both method being 96.6%The ELISA for the detection of tetanus antibodies is highly sensitive, specific and reproducible, and no animal is needed for the test. The ELISA is simpler than the animal test and provides a more convienient technique for epidemiological survey and clinical diagnosis of tetanus in most laboratories and hospitals.