5名电离辐射事故患者外周血T细胞T细胞抗原受体、T细胞分化抗原决定簇－3效应的研究

文中报道了５名事故性急性骨髓型放射病患者照后２．５和３．５年外周血Ｔ淋巴细胞Ｔ细胞受体（ＴＣＲ）基因，ＴＣＲ、Ｔ细胞分化抗原决定簇－３（ＣＤ_３）表达与ＴＣＲ／ＣＤ_３复合物功能的辐射效应．发现５名患者于照后２．５年，２名（５．２Ｇｙ和２．４Ｇｙ，５５岁）于照后３．５年外周血Ｔ细胞应答抗ＣＤ３单抗刺激而增殖的能力尚未完全恢复；经同时用ＩＬ－２和抗ＣＤ_３单抗刺激，增殖能力比单用抗ＣＤ_３单抗刺激有所增强；后２名的外周血ＴＣＲ、ＣＤ_３阳性细胞百分率一直低于正常对照和其他患者；并见一患者出现ＤＮＡ重排杂交带型．本文并从ＴＣＲ／ＣＤ_３在介导Ｔ细胞抗原刺激反应中的作用，电离辐射对ＴＣＲ／ＣＤ_３复合物的影响，后果和意义等方面进行了讨论．

Study of the bioeffects of ionization radiation on TCR and CD_3 of peripheral T-cells in 5 cases with accidental exposure

TCR gene,TCR,CD_3 expression,the function of TCR/CD_3 complex and the links between each other of them of the peripheral T-cells in 5 cases of accidental exposure to ionization radiation were studied.It was found in 2 cases 3.5 years exposure,the capacity of proliferation of the peripheral T-cells in response to the stimulation of CD_3 monoclonal antibodies(CD_3 McAb)was not recovered.The proliferative capacity of T-cells was larger when IL-2 and CD_3 McAb were used combinedly as the stimulant.In other 2 cases,the percentage of the positive cells to TCR and CD_3 was consistently lower than the control and other cases and rearranged hybrid DNA bend was seen in one case.The effect of TCR/CD_3 on the reaction of the mediation of T-cell antigen stimulation and the effect,consequence and significance of ionization radiation on TCR/CD_3 complex were discussed.