海绵共附生真菌Hansfordia sp.次级代谢产物的分离与鉴定

目的从海绵共附生真菌Hansfordia sp.的次级代谢产物中得到具有活性的化合物。方法采用硅胶柱色谱、Sephadex LH-20柱色谱和半制备HPLC色谱分离纯化,根据化合物的NMR、MS波谱数据和理化性质进行结构鉴定。结果从真菌Hansfordia sp.的固体发酵物的乙酸乙酯提取物中分离得到8个单体化合物,分别鉴定为1,3,6-trihydroxy-8-methylxanthone(1)、4-chloro-1,3,6-trihydroxy-8-methylxanthone(2)、6-hydroxymellein(3)、(R)-4ydroxymellein(4)、3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(5)、2,5-dimethyl-7-hydroxychromone(6)、(S)-regiolone(7)和cy-clonerodiol(8)。结论化合物1～8全部为首次从Hansfordia属真菌中分离得到。     

Salicylic acid derivatives and phenylspirodrimanes from the sponge-associated fungus Hansfordia sinuosae

Abstract

Three new salicylic acid derivatives (1–3) and a known one, 6-(3′-hydroxypropyl)-2-hydroxybenzoic acid (4), together with seven known phenylspirodrimanes (5–11), were isolated from the sponge-associated fungus Hansfordia sinuosae, collected from the South China Sea. HRESIMS, FT-IR Spectroscopy, and NMR techniques including COSY, HSQC, and HMBC were used to elucidate the structures of these compounds. The inhibitory effects of the isolated compounds (1–11) against HIV-1 virus were evaluated, and most of the phenylspirodrimanes (5, 8–11) showed varying degrees of anti-HIV activity.     

植物内生真菌Hansfordia sp.次生代谢产物研究

对植物内生真菌Hansfordia sp.的化学成分进行研究。采用硅胶柱、Sephadex LH-20柱和半制备HPLC色谱等手段进行分离纯化,通过波谱分析,结合文献对照,鉴定化合物结构。从植物内生真菌Hansfordiasp.中分离鉴定8个化合物,分别为:4,5-dimethoxycandidusin A(1)、prenylcandidusin C(2)、prenylcandidusin B(3)、ascotricin A(4)、3,6,8-trihydroxy-1-methylxanthone(5)、(22E,24R)-ergosta-5,7,22-trien-3β-ol(6)、stigmasta-4,6,8(14),22-tetraen-3-one(7)、(3β,5α,22E)ergosta-6,8(14),22-triene-3,5-diol(8)。化合物1~8为首次在该种菌株中分离得到。