chan皮肤鳞癌组织芯片中P53与VEGF表达的关系

目的：研究皮肤鳞癌（SSCC）组织芯片中血管内皮生长因子（VEGF，vascularendothelialgrowthfactor）的表达与p53之间的关系。方法：用免疫组化的方法研究了90例皮肤鳞癌组织芯片中VEGF、P53的表达。结果：VEGF的表达率为64．4％（58／90），p53的表达率为48．9％（44／90）。经连续切片对比分析表明，在VEGF表达较高的区域，P53的表达亦较强，X^2检验发现p53突变与VEGF表达相关（X^2=5、65，P〈0．05）。结论：在皮肤鳞癌组织芯片中．VEGF的表达与p53的表达正相关。     

山豆根水提物对体外培养人肝癌细胞增殖及代谢的影响

目的:研究中药山豆根(SSCC)提取物对体外培养的人肝癌细胞的抑癌活性。方法:SSCC经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得SSCC提取物。人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。结果:1经SSCC提取物处理48h,其A570光吸收降为对照组的62.1%,72h为9.64%;2SSCC提取物处理7d后,人肝癌细胞数明显低于对照组;3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;4经SSCC提取物处理24h后其有丝分裂指数为对照组的50.6%。结论:中药SSCC提取物能抑制体外培养的人肝癌细胞的增殖,降低线粒体代谢活性。     

Survivin基因在皮肤鳞状细胞癌中的表达及意义

目的 研究Survivin基因在皮肤鳞状细胞癌(SSCC)和正常皮肤组织中的表达情况,探讨其在SSCC发生、发展中的可能作用.方法 采用RT-PCR对38例SSCC组织中Survivin mRNA的表达进行检测,通过免疫组织化学S-P法检测60例SSCC组织中Survivin蛋白的表达,分析Survivin的表达与SSCC临床病理特征的关系.结果 RT-PCR和免疫组化结果显示Survivin mRNA及其蛋白在SSCC中高表达,且与SSCC的病理分级及淋巴结转移密切相关(P＜0.05),与患者的性别、年龄、部位无明显的相关性(P＞0.05).结论 SSCC中Survivin基因的表达能促进SSCC的发生、发展和转移,可作为判断SSCC恶性程度、评价预后的指标.     

Effects of the Primary NbC Elimination on the SSCC Resistance of a HSLA Steel for Oil Country Tubular Goods

Sulfide stress corrosion cracking (SSCC) has been of particular concern in high strength low alloyed (HSLA) steels used in the oil industry, and the non-metallic inclusions are usually considered as a detrimental factor to the SSCC resistance. In the present work, continuous casting (CC) and electroslag remelting (ESR) were adopted to fabricate a 125 ksi grade steel in order to evaluate the effect of microstructure with and without primary NbC carbides (inclusions) on the SSCC resistance in the steel. It was found that ESR could remove the primary NbC carbides, and hence, slightly increase the strength without deteriorating the SSCC resistance. The elimination of primary NbC carbides caused two opposite effects on the SSCC resistance in the studied steel. On the one hand, the elimination of primary NbC carbides increased the dislocation density and the proportion of high angle boundaries (HABs), which was not good to the SSCC resistance. On the other hand, the elimination of primary NbC carbides also induced more uniform nanosized secondary NbC carbides formed during tempering, providing many irreversible hydrogen traps. These two opposite effects on SSCC resistance due to the elimination of primary NbC carbides were assumed to be offset, and thus, the SSCC resistance was not greatly improved using ESR.     

p53和EGFr在皮肤鳞癌中表达的研究

目的：研究皮肤鳞癌（SSCC）中p53和表皮生长因子受体（EGFr）的表达，探讨其在SSCC多阶段发生中与癌增殖、分化的关系。方法：采用免疫组化(LSAB)方法研究60例SSCC中p53和EGFr的表达。结果:在正常上皮中p53表达缺乏，癌中p53阳性表达率为56.67%(34/60).EGFr表达于整个癌巢中，p53和EGFr表达在癌的不同分化有显著差异，p53和EGFr的表达两者在SSCC中表达呈显著正相关。结论：在SSCC中由免疫组化显示p53和EGFr过表达的癌细胞常是获得了更具增殖、侵袭和间变能力的细胞类型;p53和EGFr过表达是判断SSCC增殖和分化的有意义指标。