祛疡清热汤治疗中重度溃疡性结肠炎疗效及对患者血清LPO与SCD-1表达的影响

目的:探讨祛疡清热汤对中重度溃疡性结肠炎患者血清中脂质过氧化物(LPO)与硬脂酰辅酶A脱氢酶(SCD-1)表达的影响。方法: 将84例中重度溃疡性结肠炎患者作为研究对象,依照随机数字表法将患者分为观察组和对照组,每组42例。对照组给予英夫利昔单抗治疗,观察组在对照组的基础上加用祛疡清热汤治疗,对比两组患者的临床疗效及不良反应的发生情况,以及在治疗前后T细胞群、LPO及硬脂酰-LPC/油酰-LPC(SCD-1)水平的变化情况。结果:经治疗结束后,两组患者均有一定疗效(观察组90.5% 与对照组73.8%,χ²=3.977,P<0.05),且不良反应发生率较低; 经治疗结束后,两组的Treg/Th₁₇相较于治疗前明显升高,且观察组的Treg/Th₁₇显著高于对照组,差异具有统计学意义(P<0.05); 经治疗结束后,两组患者血清中LPO明显下降,硬脂酰-LPC/油酰-LPC得到升高,且观察组的LPO与硬脂酰-LPC/油酰-LPC水平显著优于对照组,差异有统计学意义(P<0.05)。结论:采用祛疡清热汤治疗中重度溃疡性结肠炎具有较好的临床疗效,可调节血清LPO与SCD-1的表达水平,增强免疫功能。     

乌司他丁对重度烧伤患者SIRS发生率和氧化应激因子水平的影响

目的:研究乌司他丁对重度烧伤患者全身炎症反应综合征(Systemic inflammatory response syndrome,SIRS)发生率和氧化应激因子水平的影响。方法:选取笔者医院2016年4月-2018年7月收治的重度烧伤患者82例,根据治疗方法不同分为两组。对照组给予清洗、补液、抗感染、抗休克及加强营养支持等基本治疗,观察组在对照组基础上给予乌司他丁治疗。分析两组患者治疗后的SIRS发生率及丙二醛(Malonaldehyde,MDA)、脂质过氧化物(Lipid peroxide,LPO)、髓过氧化物酶(Myeloperoxidase,MPO)、白细胞介素-2(Interleukin-2,IL-2)、白细胞介素-10(Interleukin-10,IL-10)表达水平。结果:观察组患者住院时间为(16.98±4.27)d短于对照组的(23.25±5.11)d,差异具有统计学意义(t=6.029,P=0.000)。两组治疗前MDA、LPO、MPO、IL-2、IL-10水平比较,差异不具有统计学意义(P>0.05)。观察组治疗3d、5d、7d后MDA、LPO、MPO水平低于对照组,IL-2、IL-10水平高于对照组,差异有统计学意义(P<0.05)。观察组入院3d、7d时SIRS发生率低于对照组,差异有统计学意义(P<0.05)。结论:乌司他丁应用于重度烧伤患者可调节IL-2、IL-10的表达水平,抑制氧化应激反应,减少SIRS发生率,缩短住院时间,加速患者康复。     

Mechanism of adrenocortical toxicity induced by quinocetone and its bidesoxy-quinocetone metabolite in porcine adrenocortical cells in vitro

Quinocetone (QCT) is a new feeding antibacterial agent in the QdNOs family. The mechanism of its adrenal toxicity is far from clear. This study was conducted to estimate the adrenal cell damage induced by QCT and its bidesoxy-quinocetone (B-QCT) metabolite and to further investigate their mechanisms. Following doses of QCT increasing from 5 to 50 μM, cell apoptosis and necrosis, mitochondrial dysfunction and redox imbalance were observed in porcine adrenocortical cells. The mRNA levels of the six components of intermediary enzymes and the adrenal renin-angiotensin-aldosterone system (RAAS) displayed a dysregulation induced by QCT, indicating that QCT might influence aldosterone secretion not only through the upstream of the production but also through the downstream of the adrenal RAAS pathway. In contrast, B-QCT had few toxic effects on the cell apoptosis, mitochondrial dysfunction and redox imbalance. Moreover, LCMS-IT-TOF analysis showed that no desoxy metabolites of QCT were found in either cell lysate or supernatant samples. In conclusion, we reported on the cytotoxicity in porcine adrenocortical cells exposed to QCT via oxidative stress, which raised awareness that its toxic effects resulted from N→O groups, and its toxic mechanism might involve the interference of the steroid hormone biosynthesis pathway.     

Effect of two monoterpene phenols on antioxidant defense system in Candida albicans

Thymol and carvacrol from the class of monoterpene phenols are one of the most potent plant essential oil components possessing antimicrobial effects. Known for their wide bioactive spectrum, these positional isomers of isopropyl cresol deplete ergosterol content, compromise membrane permeability, block efflux pumps and restore antifungal susceptibility to fluconazole in resistant Candida strains. Exposure to these natural compounds induces a cascade of stress responses, which are important to comprehend their microbicidal mechanisms. This study evaluates the antioxidant defense response to lower concentrations of thymol and carvacrol in Candida albicans. The antioxidant defense responses in C. albicans are important for developmental mechanisms pertaining to resistance against the immune system, infection establishment and drug resistance. In this view, primary and secondary antioxidant defense enzymes, and oxidative stress markers including glutathione and lipid peroxidation were determined in C. albicans cells exposed to lower concentrations of thymol and carvacrol. These compounds were found to induce oxidative stress and compromised the antioxidant defense system in C. albicans at lower concentrations. This study helps in understanding the ‘in cell’ antifungal mechanisms of natural monoterpene phenols originating from oxidative stress. Thymol and carvacrol induced membrane deterioration reported earlier, is further explained as a result of a toxic radical cascade mediated by lipid peroxidation. Findings reinforce the observed toxic oxidizing effects of these compounds as a consequence of direct damage to antioxidant components and not to their genetic manipulations.     

胃得安胶囊联合兰索拉唑治疗胃溃疡的临床研究

目的探讨胃得安胶囊联合兰索拉唑治疗胃溃疡的临床效果。方法选取2016年7月—2018年7月天津市第五中心医院收治的胃溃疡患者96例,随机分成对照组(48例)和治疗组(48例)。对照组早餐前30 min口服兰索拉唑肠溶胶囊,30 mg/次,1次/d。治疗组在对照组基础上口服胃得安胶囊,2粒/次,3次/d。两组患者均连续治疗6周。观察两组患者临床疗效,同时比较治疗前后两组患者上腹痛评分、反酸评分、前列腺素(PG)E2、C反应蛋白(CRP)、白介素-17(IL-17)、超氧化物歧化酶(SOD)和脂质过氧化物(LPO)水平以及幽门螺杆菌根除率、溃疡愈合率及复发率。结果治疗后,对照组临床有效率为87.5%,显著低于治疗组的100.0%,两组比较差异有统计学意义(P0.05)。治疗后,两组患者中上腹痛评分和反酸评分均显著降低(P0.05),且治疗组患者中上腹痛评分和反酸评分明显低于对照组(P0.05)。治疗后,两组患者胃液中PGE2浓度及血清SOD水平均显著升高(P0.05),血清CRP、IL-17、LPO浓度则均显著降低(P0.05),且治疗组患者这些指标水平明显好于对照组(P0.05)。治疗后,治疗组幽门螺杆菌根除率、溃疡愈合率分别为93.8%、75.0%,均显著高于对照组(P0.05)。随访6个月,治疗组复发率为8.3%,比对照组的25%明显显著降低(P0.05)。结论胃得安胶囊联合兰索拉唑治疗胃溃疡的整体疗效切实,促进溃疡愈合,增强胃黏膜的防御功能及机体的抗氧化能力。     

脑复康对中老年认知功能和LPO影响的观察

选择认知功能减退患者２１例连服脑复康２个月，经治疗前后韦氏记忆量表（ＷＭＳ）和血过氧化脂质（ＬＰＯ）检测显示：使ＷＭＳ１０项目中的６项及量表总分、记忆商值均显著提高（Ｐ＜０．０１或＜０．０５），表明本药有改善记忆障碍的作用；使ＬＰＯ明显下降（Ｐ＜０．０５），对防治老年疾病的发生和延缓机体衰老具有一定意义。     

奥曲肤对急性胰腺炎患者白细胞介素及LPO、 CRP、NF-α水平影响的研究

目的探讨奥曲肽对急性胰腺炎患者多项白细胞介素及LPO、CRP、TNF-α水平影响。方法选取2007年1月～2010年5月进行治疗的35例急性胰腺炎为研究对象,将其设为观察组,采用奥曲肽进行治疗,同时选取同期的35名健康人为对照组,分别于治疗前后7d及14d对观察组及对照组的血清IL-6、IL-8、IL-10及LPO、CRP、TNF-α水平进行检测及比较。结果治疗前观察组的血清IL-6、IL-8、IL-10及LPO、CRP、TNF-α水平均显著高于对照组,P均<0.01,差异均有统计学意义,而治疗后7d观察组的血清IL-6、IL-8、IL-10及LPO、CRP、TNF-α水平与治疗前比较,P均<0.05,差异均有统计学意义,治疗14d时与对照组比较,P均>0.05,差异均无统计学意义。结论奥曲肽对急性胰腺炎患者多项白细胞介素及LPO、CRP、TNF-α水平影响较大,可显著降低这些血清因子的水平,对疾病的转归有积极的意义。     

丹参对家兔实验性动脉粥样硬化预防作用的研究

丹参预防组主动脉斑块面积占主动脉总面积的百分比为（１９．８５±１１．７９％），明显小于动脉粥样硬化（ＡＳ）模型组（３７．７９±１０．８０％），Ｐ＜０．０５。丹参有显著保护超氧化物歧化酶（ＳＯＤ）活性和清除脂质过氧化物中间产物丙二醛（ＭＤＡ）的作用。丹参能明显抑制在高脂条件下３Ｈ－ＴｄＲ掺入培养的平滑肌（ＳＭＣ），抑制ＳＭＣ、ＤＮＡ合成。本研究初步探讨了丹参抑制家兔实验性ＡＳ病变发生发展的作用机制。     

Fisetin, a novel flavonol attenuates benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice

Lung cancer is the foremost cause of cancer mortality and is a growing economic burden worldwide. Fisetin (3,7,3′,4′-tetrahydroxyflavone), a naturally occurring flavonoid is found in vegetables and fruits possesses anti-oxidative, anti-inflammatory and anti-proliferative effects in a wide variety of cancer. In the present study it is hypothesized that fisetin may provide chemopreventive as well as chemotherapeutic effects against experimental lung carcinogenesis. The present study was designed to investigate whether fisetin confers anti-cancer action against benzo(a)pyrene [B(a)P] induced lung carcinogenesis. Treatment with fisetin significantly reduced the degree of histological lesions, restored the levels of lipid peroxidation (LPO), enzymic and non-enzymic anti-oxidants in B(a)P-induced mice. Anti-proliferative efficacy of fisetin was assessed by immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) in B(a)P induced mice showed increased PCNA expression which is restored upon fisetin administration. Together, our results depicts that fisetin can be used as chemopreventive agent against lung cancer.     

Protective effect of gallic acid against lindane induced toxicity in experimental rats

Lindane is an organochlorine pesticide that persists in the environment, bioaccumulate through food chain and has a risk of causing adverse effects to human health and the environment. It induces cell damage by producing free radicals and reactive oxygen species. The aim of the present study is to investigate the protective effect of gallic acid (a plant derived polyphenol) against lindane induced hepatic and renal toxicity in rats. Liver damage was assessed by hepatic serum marker enzymes like SGOT, SGPT and ALP and histopathological observation. Renal damage was observed by histopathological examination and serum markers like creatinine and urea. Treatment with lindane increased the levels of lipid peroxidation, serum marker enzyme activity with a concomitant decrease in GSH, CAT, SOD, GPx and GST. Histological alterations were also observed in kidney and liver tissue with lindane treatment. Co-treatment of gallic acid significantly prevented the lindane induced alterations in kidney and liver tissues with a decrease in LPO, serum marker enzyme activity and a significant increase in antioxidant levels. These results suggest that gallic acid has protective effect over lindane induced oxidative damage in rat liver and kidney.