气相色谱法测定冠心苏合胶囊中土木香内酯和异土木香内酯的含量

目的:建立冠心苏合胶囊中土木香内酯和异土木香内酯的含量的测定方法。方法:采用聚乙二醇20 000(PEG-20M)毛细管柱,FID检测器,程序升温:初始温度190℃,保持30 min,以120℃.min-1的速率升温至240℃,保持5 min;进样口温度260℃;检测器温度280℃。结果:在该色谱条件下,土木香内酯在48.96～244.8 mg.L-1呈良好的线性关系,异土木香内酯在51.28～256.4 mg.L-1呈良好的线性关系。结论:本方法灵敏、准确、重复性好,可用于冠心苏合胶囊的质量控制。     

冠心苏合丸特征图谱研究

目的建立一种全面控制冠心苏合丸药品质量的方法。方法以混合对照药材作为对照,用气相色谱法进行样品分析,从而确立冠心苏合丸特征鉴别图谱。色谱条件:HP-5弹性石英毛细管柱(30 m×0.25 mm,0.25μm),程序升温,FID检测器。结果建立了冠心苏合丸特征鉴别图谱,各味药材互不干扰。样品色谱图显示与特征图谱保留时间一致的特征峰。结论该方法重复性好,操作简便,易于推广,可全面控制药品质量。     

Occupational contact dermatitis from Cichorium (chicory, endive) and Lactuca (lettuce)

In two cases, occupational contact dermatitis was found to be due to chicory (Cichorium) used as a salad plant. In one of the two cases, contact sensitivity to letuce (Lactuca) was also observed. The sesquiterpene lactones of the plant may be the allergens.     

HPLC同时测定三味甘露散中土木香内酯和异土木香内酯含量

目的建立同时测定三味甘露散中土木香内酯、异土木香内酯含量的方法。方法采用高效液相色谱法,光电二极管阵列检测器,色谱柱为CAPCELL PAK MG C18(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸(58∶42),流速1.0 mL/min,检测波长225 nm,柱温30℃。结果土木香内酯在0.083~0.517μg、异土木香内酯在0.108~0.672μg范围内呈良好线性关系,r=0.999 9;土木香内酯平均加样回收率为97.95%,RSD=1.31%;异土木香内酯平均加样回收率为97.69%,RSD=1.24%。结论本方法操作简便,可快速、准确地测定三味甘露散中土木香内酯和异土木香内酯的含量。     

土木香内酯抗病毒作用的体内外研究

目的 探索土木香内酯体内外的抗病毒作用,并基于Ⅰ型干扰素(type Ⅰ interferon,IFN-Ⅰ)通路探究其抗病毒作用机制。方法 CCK-8法检测土木香内酯对A549细胞活力的影响;利用表达绿色荧光蛋白的水疱性口炎病毒(vesicular stomatitis virus expressing green fluorescent protein,VSV-GFP)感染细胞模型,结合流式细胞术检测土木香内酯对病毒复制的影响;qRT-PCR检测土木香内酯对甲型流感病毒(influenza A virus,H1N1)、脑心肌炎病毒(encephalomyocarditis virus,EMCV)复制的影响;Western blotting检测土木香内酯对VSV病毒G蛋白和H1N1病毒NP蛋白表达的影响;利用生物信息学初步分析土木香内酯的抗病毒分子机制;qRT-PCR检测药物处理后干扰素α1(interferon α1,Ifna1)、干扰素β1(interferon β1,Ifnb1)、干扰素诱导蛋白1(interferon-induced protein with tetratrico...     

土木香趁鲜切制与传统切片方法的比较

目的: 探讨鲜切土木香饮片的可行性,对土木香传统切片工艺进行改良。 方法: 以土木香内酯、异土木香内酯及总黄酮含量为指标,通过正交试验考察含水量、干燥方式及切片厚度对土木香直接鲜切饮片工艺的影响,并与传统切片工艺进行比较。采用紫外分光光度法测定总黄酮含量;HPLC测定土木香内酯和异土木香内酯含量,色谱条件为SHIMADZU C₁₈色谱柱(4.6 mm×200 mm,5 μm),流动相乙腈-0.1% 磷酸溶液(50:50),柱温30 ℃,流速1.0 mL·min^-1,检测波长220 nm,进样量20 μL。 结果: 优选的鲜切饮片工艺为土木香药材在含水量为35% 时切片,饮片厚度2.5~3.0 mm,阴干。鲜切饮片中土木香内酯、异土木香内酯及总黄酮质量分数分别为(22.41±1.38),(12.87±0.73),(15.04±0.64) mg·g^-1,传统工艺切制饮片中则依次为(22.94±1.24),(13.24±0.83),(14.12±0.58) mg·g^-1。 结论: 趁鲜切制土木香饮片产品质量稳定,具有推广意义。     

Alantolactone and Isoalantolactone Prevent Amyloid β25–35‐induced Toxicity in Mouse Cortical Neurons and Scopolamine‐induced Cognitive Impairment in Mice

Given the evidence for detoxifying/antioxidant enzyme‐inducing activities by alantolactone (AL) and isoalantolactone (IAL), the purpose of this study was to investigate the effects of AL and IAL on Aβ_25–35‐induced cell death in mouse cortical neuron cells and to determine their effects on scopolamine‐induced amnesia in mice. Our data demonstrated that both compounds effectively attenuated the cytotoxicity of Aβ_25–35 (10 μM) in neuronal cells derived from the mouse cerebral cortex. It was also found that the production of intracellular reactive oxygen species, including superoxide anion induced by Aβ_25–35, was inhibited. Moreover, the administration of the sesquiterpenes reversed scopolamine‐induced cognitive impairments as assessed by Morris water, Y‐maze, and the passive avoidance tests, and the compounds decreased acetylcholinesterase (AChE) activities in a dose‐dependent manner. Interestingly, AL and IAL did not improve scopolamine‐induced cognitive deficit in Nrf2 ^?/? mice, suggesting that memory improvement by sesquiterpenes was mediated not only by the activation of the Nrf2 signaling pathway but also by their inhibitory activity against AChE. In conclusion, our results showed that AL and IAL had neuroprotective effects and reversed cognitive impairments induced by scopolamine in a mouse model. Therefore, AL and IAL deserve further study as potential therapeutic agents for reactive oxygen species‐related neurodegenerative diseases. Copyright © 2017 John Wiley & Sons, Ltd.