A strain of Aspergillus fumigatus has been isolated from sediments of a mussel bed. When cultured in hyper saline conditions (with sea-water), it produces a cytotoxic and immunosuppressive toxin, gliotoxin, which is excreted in an exudate. In order to know if this toxin could represent a risk for shellfish consumers, an experiment of bioaccumulation of gliotoxin in mussel has been carried out. After 6 days of contamination, toxin was accumulated in the meat of the mussels, at a level up to 2.9 microg/mg of extract weight, with a mode of contamination different to the classical digestive process described for a majority of marine toxins, but similar to the contamination mode of domoic acid. 相似文献
Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%~99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity. 相似文献
It could be demonstrated that morphological changes in bone cement are different in fibroblasts and osteoblasts. Osteoblasts seem to grow on bone cement and develop an orderly formation. Fibroblasts grow in a confluent monolayer around bone cement but do not adhere to the cement itself. This is a desirable effect since most Orthopaedic applications aim at osteointegration as opposed to fibrous tissue overgrowth. Apoptosis attributed to bone cement is comparable to the respective natural rate of apoptosis. Cyanoacrylate glue and the mussel adhesive protein lead to an almost complete apoptosis in the investigated cells. Their routine application should be avoided. The developed cell culture model seems appropriate for performing further investigations. 相似文献