西洋参茎叶皂甙对 CPHD病人外周淋巴细胞增强与调节作用

目的 :为了开发西洋参在临床医学方面的应用 ,提高CPHD病人的各项免疫指标。方法 :选择 4 0例CPHD病人 ,分成用药组 2 0例 ,对照组 (非用药组 ) 2 0例 ,通过流式细胞术检测病人外周血中T淋巴细胞亚群中各亚群所占的比例来反映机体的细胞免疫状态。结果 :通过两组临床研究观察 ,用药组疗效优于对照组 (P <0 0 5 )。西洋参茎叶皂甙可直接作用于淋巴细胞分化成熟过程 ,具有增强和调节CPHD病人机体免疫功能的作用。结论 :合理应用西洋参茎叶茎叶皂甙对改善CPHD病人免疫功能低下和紊乱状态 ,具有重要临床意义。     

PQS抑制模拟缺血-再灌注心肌细胞FAS的表达

目的探讨西洋参总皂苷(PQS)对缺血再灌注诱导心肌细胞凋亡的FAS mRNA的表达影响。方法利用原代培养的乳鼠心肌细胞模拟缺血模型2h,再恢复正常培养24h;RT-PCR及原位杂交检测PQS处理后FAS mRNA水平。结果 PQS能显著抑制缺血再灌注Fas的表达。结论实验表明PQS能抑制心肌细胞缺血再灌注FAS凋亡基因的表达。     

西洋参茎叶皂甙对低频电刺激豚鼠乳头肌的抗缺糖缺氧作用

西洋参茎叶皂甙对豚鼠乳头肌电刺激频率在1/8～1/64Ｈｚ，可增强其收缩力（Ｐ＜０．００５）。在营养液中加入５０ｍｏ１／Ｌ葡萄糖并充以95%N₂和5%CO₂，或在营养液中无糖并充以95%N₂和5%CO₂时，在低频刺激（1/8～1/64Hz）时西洋参茎叶皂贰具有抗缺氧缺糖作用（p<0.05，p<0.01）。     

西洋参茎叶皂甙对某些免疫功能影响的实验研究

作者从西洋参茎叶中提得总皂甙(panaxquinquefolium sapoin)简称PQS,观察了PQS对免疫功能的影响。结果PQS体内给药8天后100mg/kg组T、B淋巴细胞转化率显著高于对照组(P<0.05);可促进conA诱导IL—2产生(P<0.05);脾脏T细胞百分率及B细胞的抗体产生水平均显著高于对照组(P<0.01),说明在100mg/kg剂量下,机体的T、B淋巴细胞功能都有明显的增强作用。     

Community surveillance enhances Pseudomonas aeruginosa virulence during polymicrobial infection

Most infections result from colonization by more than one microbe. Within such polymicrobial infections, microbes often display synergistic interactions that result in increased disease severity. Although many clinical studies have documented the occurrence of synergy in polymicrobial infections, little is known about the underlying molecular mechanisms. A prominent pathogen in many polymicrobial infections is Pseudomonas aeruginosa, a Gram-negative bacterium that displays enhanced virulence during coculture with Gram-positive bacteria. In this study we discovered that during coinfection, P. aeruginosa uses peptidoglycan shed by Gram-positive bacteria as a cue to stimulate production of multiple extracellular factors that possess lytic activity against prokaryotic and eukaryotic cells. Consequently, P. aeruginosa displays enhanced virulence in a Drosophila model of infection when cocultured with Gram-positive bacteria. Inactivation of a gene (PA0601) required for peptidoglycan sensing mitigated this phenotype. Using Drosophila and murine models of infection, we also show that peptidoglycan sensing results in P. aeruginosa-mediated reduction in the Gram-positive flora in the infection site. Our data suggest that P. aeruginosa has evolved a mechanism to survey the microbial community and respond to Gram-positive produced peptidoglycan through production of antimicrobials and toxins that not only modify the composition of the community but also enhance host killing. Additionally, our results suggest that therapeutic strategies targeting Gram-positive bacteria might be a viable approach for reducing the severity of P. aeruginosa polymicrobial infections.     

西洋参茎叶皂甙湖养的大鼠心肌细胞动作电位的影响

西洋参茎叶皂甙(PQS250、500μg/ml)使培养的Wistar大鼠心肌细胞动作电位的波幅、波宽、阈电位、最大舒张电位、最大除极速度及复极50％水平的动作电位波宽一致减小。实验结果表明,ROS可能具有钙通道阻滞作用。     

西洋参茎叶皂甙对小鼠学习记忆的易化作用

西洋参茎叶皂甙500mg/kg、100mg／kg给小鼠连续灌胃7天，可拮抗樟柳碱和戊巴比妥钠引起的记忆获得损害，对环己酰亚胺引起的记忆巩固障碍有显著改善作用。西洋参茎叶皂甙还可使常压、低压缺氧状态下小鼠存活时间有所延长。     

西洋参茎叶皂苷对CPHD患者细胞免疫功能的影响

目的　通过观察西洋参茎叶皂苷对慢性肺原性心脏病 (CPHD)患者细胞免疫功能的影响 ,探讨CPHD患者的发病与机体免疫的关系。方法　采用流式细胞术检测 2 0例CPHD患者外周血T淋巴细胞亚群和NK(CD3-/CD16 +5 6 + )细胞 ;RT PCR方法检测IL 2、IFNγmRNA的表达。结果　CPHD患者CD3+ 、CD4 + 、和CD4 /CD8比值明显低于对照组并差异有显著性 ,而CD8+ 细胞高于对照组 (P <0 0 1)。PQS治疗后CD3+ 、CD4 + 、CD3-/CD16 +5 6 + 细胞和CD4 /CD8比值都升高 ,其中CD4 + 、CD3+ 细胞与治疗前比较呈显著性差异 (P <0 0 5 ) ,而CD8+ 细胞与治疗前比较降低 (P <0 0 5 )。西洋参茎叶皂苷 (PQS)能促进T细胞分泌细胞因子IL 2、IFNγmRNA的表达。结论　当CPHD患者免疫功能低下时 ,选用PQS治疗能够提高机体的细胞免疫功能     

西洋参茎叶皂甙对大鼠培养心肌细胞氧化损伤的保护作用

黄嘌呤-黄嘌呤氧化酶可引起大鼠培养心肌细胞电参数变小、心肌细胞起搏比例减少及心肌细胞超微结构破坏。西洋参茎叶皂甙可使上述指标明显改善,说明其有抗氧化损伤作用。     

G2 arrest and apoptosis by 2-amino-N-quinoline-8-yl-benzenesulfonamide (QBS), a novel cytotoxic compound

We screened a library of 11,000 small molecular weight chemicals, looking for compounds that affect cell viability. We have identified 2-amino-N-quinoline-8-yl-benzenesulfonamide (QBS) as a potent cytotoxic compound that induces cell cycle arrest and apoptosis. Treatment of Jurkat T cells with QBS increased the levels of cyclin B1 as well as phosphorylated-cdc2, which was accompanied by reduced activity of cdc2 kinase, suggesting that QBS may induce cell cycle arrest at G2 phase. Structural analogues of QBS also exhibited similar effects on cell cycle progression and cell viability. Long-term treatment with QBS resulted in DNA fragmentation, cytochrome C release, and PARP cleavage, and an increase in the number of subdiploidy cells, indicative of cellular apoptosis. Moreover, QBS-induced apoptosis was blocked by z-VAD-fmk, a pan-caspase inhibitor. These results suggest that QBS is a novel and potent compound that induces G2 arrest and subsequent apoptosis, implicating it as a putative candidate for chemotherapy.