全文获取类型
收费全文 | 518篇 |
免费 | 61篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 54篇 |
儿科学 | 10篇 |
妇产科学 | 31篇 |
基础医学 | 89篇 |
口腔科学 | 9篇 |
临床医学 | 48篇 |
内科学 | 82篇 |
皮肤病学 | 4篇 |
神经病学 | 18篇 |
特种医学 | 35篇 |
外科学 | 70篇 |
预防医学 | 7篇 |
眼科学 | 2篇 |
药学 | 14篇 |
肿瘤学 | 107篇 |
出版年
2023年 | 3篇 |
2021年 | 11篇 |
2020年 | 13篇 |
2019年 | 12篇 |
2018年 | 11篇 |
2017年 | 22篇 |
2016年 | 32篇 |
2015年 | 26篇 |
2014年 | 33篇 |
2013年 | 27篇 |
2012年 | 52篇 |
2011年 | 45篇 |
2010年 | 38篇 |
2009年 | 28篇 |
2008年 | 26篇 |
2007年 | 16篇 |
2006年 | 18篇 |
2005年 | 20篇 |
2004年 | 17篇 |
2003年 | 18篇 |
2002年 | 20篇 |
2001年 | 5篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1977年 | 1篇 |
1976年 | 4篇 |
1975年 | 1篇 |
1974年 | 4篇 |
1973年 | 5篇 |
1972年 | 1篇 |
1971年 | 6篇 |
1970年 | 13篇 |
1969年 | 3篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1937年 | 1篇 |
1928年 | 1篇 |
排序方式: 共有580条查询结果,搜索用时 15 毫秒
1.
Piotr Podolec Grzegorz Kope? Jakub Podolec Piotr Wilko?ek Marek Krochin Pawe? Rubi? Marcin Cwynar Tomasz Grodzicki Krzysztof Zmudka Wies?awa Tracz 《Hypertension research》2007,30(12):1151-1158
The objectives of this study were to determine the relationship between carotid-femoral (cfPWV) and aortic pulse wave velocity (aPWV) and to compare their modulators and association with coronary artery disease (CAD). We studied 107 consecutive patients (68 men) with a mean age of 60.49+/-8.31 years who had stable angina and had been referred for coronary angiography. cfPWV and aPWV were measured simultaneously during cardiac catheterization using the Complior device and aortic pressure waveform recordings, respectively. Based on the presence or absence of significant coronary artery stenosis (CAS) patients were subdivided into a CAS+ or CAS- group. The mean values of cfPWV and aPWV were 10.65+/-2.29 m/s and 8.78+/-2.24 m/s, respectively. They were significantly higher in the CAS+ (n=71) compared with the CAS- (n=36) group and predicted significant CAS independently of cardiovascular risk factors and mean or systolic aortic blood pressure. aPWV and cfPWV were significantly correlated (r=0.70; p<0.001) but the degree of correlation differed significantly (p<0.03) between the CAS+ (r=0.74, p<0.001) and CAS- group (r=0.46, p=0.003). Age and mean aortic blood pressure were independent predictors for aPWV as well as cfPWV. In the receiver operating characteristic (ROC) analysis, aPWV and cfPWV had similar accuracy in identification of significant CAS (AUC [area under the ROC curve]=0.76 and 0.69, respectively; p=0.13). However, neither cfPWV nor aPWV was effective at differentiating the extent of CAD. In conclusion, aPWV and cfPWV are highly correlated parameters with similar determinants and comparable accuracy in predicting significant CAS. The strength of correlation between these two indices differed significantly between subjects with and those without CAS. 相似文献
2.
The MTT-assay as a rapid test for cell proliferation and cell killing: application to human peripheral blood lymphocytes (PBL). 总被引:1,自引:0,他引:1
The possibility to use the colorimetric MTT assay for measuring proliferation and cell death of human peripheral blood lymphocytes (PBL) was studied. In a range from 100,000-800,000 cells/well a linear correlation between the optical signal (OD signal at 570 nm) and the cell number was found. It is necessary to incubate the cells with the MTT at least 2 hours. After stimulation by different PHA concentrations a very good correlation between [3H] thymidine incorporation and MTT assay was found. A comparison of daunomycin cytotoxicity, measurement by trypan blue exclusion and MTT assay, gave also a good correlation between both methods. It can be pronounced that the MTT assay is a suitable method to measure cell proliferation and cell death of human PBL. The assay is easy to handle, a large number of probes can be assayed in a relatively short time and no radioactivity is necessary. For the measurement of the colored product a common ELISA reader can be used. 相似文献
3.
Heinrich Heimann Norbert Bornfeld Wilko Friedrichs Horst Helbig Ulrich Kellner Ayman Korra Michael H. Foerster 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》1996,234(9):561-568
Background: Pars planta vitrectomy has evolved as an alternative method in the treatment of more complicated rhegmatogenous retinal detachments. We report a series of patients who underwent primary vitrectomy with gas tamponade without the use of additional scleral buckling. Methods: A retrospective study of 53 patients with a follow-up of 6–45 months (mean 17.8 months) was carried out. Preoperative findings included unusual, multiple or large breaks, vitreous haemorrhage, proliferative vitreoretinopathy and bullous retinal detachment. Preoperative visual acuity was between light perception and 1.0, with 30% (16/53) of patients with 0.4 or better. Results: Retinal reattachment was achieved in 64% of cases (34/53) with one and in 92% (49/53) with one or more operations. Final visual acuity was between light perception and 1.0, with 41% (22/53) of patients with 0.4 or better. Cataract formation occurred in 86% (37/43) of all patients with a clear lens preoperatively. Macular pucker was noted in 11 % (6/53) and postoperative proliferative vitreoretinopathy causing redetachment in 6% (3/53). Conclusion: With primary vitrectomy, a high final anatomical success rate with few intraoperative complications can be achieved in more complicated forms of rhegmatogenous retinal detachment. The major drawback of the procedure is the high incidence of post-operative cataract formation. 相似文献
4.
Carsten Denkert Wilko Weichert Klaus-Jürgen Winzer Berit-Maria Müller Aurelia Noske Silvia Niesporek Glen Kristiansen Hans Guski Manfred Dietel Steffen Hauptmann 《Clinical cancer research》2004,10(16):5580-5586
PURPOSE: The human ELAV (embryonic lethal abnormal vision)-like protein HuR stabilizes a certain group of cellular mRNAs that contain AU-rich elements in their 3'-untranslated region. Cell culture studies have shown that the mRNA of cyclooxygenase (COX)-2 can be stabilized by HuR. EXPERIMENTAL DESIGN: To investigate a possible contribution of dysregulation of mRNA stability to the progression of cancer and to overexpression of COX-2, we studied expression of HuR in 208 primary breast carcinomas by immunohistochemistry. RESULTS: There were two different staining patterns of HuR in tumor tissue of breast carcinomas: nuclear expression was seen in 61% of cases; and an additional cytoplasmic expression was seen in 30% of cases. Expression of HuR was significantly associated with increased COX-2 expression; this association was particularly significant for cytoplasmic HuR expression (P < 0.0005). We further observed a significant association of cytoplasmic (P = 0.002) or nuclear HuR (P = 0.027) expression with increased tumor grade. Only 13% of the grade 1 carcinomas showed cytoplasmic expression of HuR, compared with 46% of the grade 3 carcinomas. There was no significant correlation between HuR expression and other clinicopathological parameters such as histological type, tumor size, or nodal status as well as patient survival. CONCLUSIONS: Our results suggest that overexpression of HuR in tumor tissue may be part of a regulatory pathway that controls the mRNA stability of several important targets in tumor biology, such as COX-2. Based on our results, additional studies are necessary to investigate whether HuR might be a potential target for molecular tumor therapy. 相似文献
5.
6.
Franziska Erlmeier Wilko Weichert Andres Jan Schrader Michael Autenrieth Arndt Hartmann Sandra Steffens Philipp Ivanyi 《Medical oncology (Northwood, London, England)》2017,34(6):99
Programmed death-1 receptor (PD-1) and programmed death-1 receptor-ligand (PD-L1) have been suggested to play a role as prognostic markers in clear cell renal cell carcinoma (ccRCC). The association between PD-L1 and prognosis seems to be more robust than for PD-1. Further, preliminary analyses suggest that neither PD-1 nor its ligands play a role as prognostic markers in non-clear cell RCC, while the prognostic role of PD-L2 in ccRCC as well as in non-clear cell RCC remains unclear. 相似文献
7.
8.
9.
Tumour budding activity and cell nest size determine patient outcome in oral squamous cell carcinoma: proposal for an adjusted grading system 下载免费PDF全文
10.