针刺疗法在脑卒中恢复期中的作用

虽然现代医学的发展大大降低了脑卒中急性期的病死率,但经积极抢救后患者往往留有较多的功能障碍。进入恢复期后,若治疗不当或不及时会给患者留有不同程度的后遗症,严重影响其生活质量。一般情况下发病2周内为急性期,2周以后进入恢复期,半年以上则为后遗症期。针刺疗法在恢复期中占有十分重要的位置,针刺能显著改善瘫痪肢体和提高语言能力等功能,     

实验性脑缺血后主要炎症相关信号的传递和作用关系

本文对参与炎症的细胞(中性粒细胞、淋巴细胞、单核细胞、巨噬细胞、内皮细胞、血管平滑肌细胞、胶质细胞)、主要产物炎症因子(IL-1、IL-6、TNF-α、ICAM-1)及细胞外基质(ECM)等在脑缺血后相互协调、相互作用的过程进行综述。     

针刺对脑缺血再灌注损伤大鼠双侧脑组织白细胞介素-1β、肿瘤坏死因子-α表达的影响

目的:探讨脑缺血再灌注损伤大鼠双侧脑组织白细胞介素-1β(IL-1β)及肿瘤坏死因子-α(TNF-α)的表达变化及针刺的干预作用。方法:以右侧大脑中动脉线栓法制备脑缺血再灌注损伤大鼠模型,将大鼠随机分为模型组、假手术组和针刺组,每组再按照再灌注后时间分12、24、48、72、96、144h6个时间点,每个时间点6只大鼠,另设正常组6只。针刺组电针刺激"百会"和"足三里"穴,每日1次,每次20min。应用免疫组化法检测大鼠双侧脑组织IL-1β及TNF-α的表达量。结果:IL-1β在脑缺血大鼠患侧脑区呈双峰表达,模型组峰值时间为48h和96h,针刺组为72h,针刺组IL-1β表达量显著低于模型组,但高于假手术组和正常组(均P<0.05);IL-1β在脑缺血大鼠健侧脑区表达高于假手术组和正常组,除72h组外,针刺组各个时间点的表达低于模型组(均P<0.05)。TNF-α在患侧脑区呈单峰表达,模型组和针刺组峰值时间均为72h,针刺组表达量显著低于模型组,但高于假手术组和正常组(均P<0.05);TNF-α在脑缺血大鼠健侧脑区表达高于假手术组和正常组(均P<0.05)。结论:针刺可通过调节脑缺血大鼠双侧脑组织IL-1β及TNF-α的表达,干预脑缺血再灌注损伤大鼠的炎性反应。     

针刺干预对脑缺血再灌注损伤大鼠外周血清蛋白表达的影响

目的:探讨脑缺血再灌注损伤模型大鼠外周血清蛋白表达的变化及针刺百会、足三里穴的干预作用。方法:以大脑中动脉线栓法制备大鼠脑缺血再灌注模型,采用随机区组设计将大鼠分为模型组、假手术组和针刺组3个大组,每大组再按照再灌注后时间分成12 h、24 h、48 h、72 h、96 h和144 h等6个亚组;每个亚组有大鼠7只;另设正常对照组7只。应用SELD I-TOF-MS检测各组大鼠外周血清蛋白表达的差异。结果:模型组与正常对照组比较,有150个差异蛋白峰(P<0.05);针刺组与模型组结果比较,有89个差异蛋白峰出现显著性变化,包括正向峰32个和负向峰57个;模型组血清蛋白峰值的分布呈双峰型,针刺干预使第一峰峰值降低,第二峰峰值时间前移。结论:针刺百会和足三里穴能够通过影响脑缺血再灌注损伤大鼠外周血清蛋白的表达,干预缺血再灌注模型大鼠的病理生理过程。     

B型超声诊断带器妊娠及其合并症

带器妊娠并非少见，及其并发症较为罕见。Ｂ型超声诊断有特异性，本文报告在早、中期妊娠Ｂ超检查中发现。带器妊娠１５例，妊娠合并金属圈环１４例，其中１０例为早孕作者单位：４５３６００河南省辉县市第二人民医院期间作人工流产取出避孕器，１例带器妊娠合并卵巢囊肿...     

针刺对大鼠脑缺血再灌注损伤后不同时间点血清SOD和MDA表达的影响!

目的:探讨针刺百会、足三里穴对脑缺血再灌注损伤大鼠不同时间点血清SOD、MDA表达的干预作用。方法:将SD大鼠随机分为假手术组、模型组、电针组和药物组。后3组制作脑缺血再灌注模型,根据再灌注时间分为1天、3天、5天和7天组。电针组针刺百会、左足三里,日1次/20 min,药物组腹腔注射依达拉奉3 mg/kg,日1次。黄嘌呤氧化酶法测血清SOD活力,TBA法测血清MDA含量。结果:模型组SOD活性先降低,后升高,低于同时间点的电针组和药物组;MDA含量先升高,后降低,高于同时间点的电针组和药物组;电针组SOD活性低于同时间点的药物组,MDA含量高于同时间点的药物组。结论:针刺大鼠百会、足三里穴可能通过上调SOD、下调MDA表达干预脑缺血再灌注损伤后氧化应激和脂质过氧化过程。     

针灸脑缺血再灌注损伤大鼠百会及足三里穴位对热休克蛋白70及肿瘤坏死因子-α表达的作用

Objective:To explore the effects of acupuncture on the peripheral serum expression of heat shock protein 70(HSP70)and tumor necrosis factor a(TNF-α)in rats with cerebral ischemia-reperfusion injury(CIRI).Methods:In total,152 Sprague-Dawley(SD)rats were randomly divided into an operated group and a non-operated group according to a random digits table.The operated group included a sham-operated group,a model group and an acupuncture group,whereas the non-operated group consisted of a normal group.Except for the normal group,each group was further divided into 12,24,48,72,96,and 144 h time points according to different reperfusion times.Eight rats were assigned in each operated group and in the normal group.The rat model of CIRI was established by the thread occlusion method in the model and acupuncture groups.The acupuncture group was treated with electroacupuncture at Baihui(DU20)and Zusanli(ST36)for the required time after successful operation.Blood was sampled to detect the HSP70 and TNF-αcontent by enzyme linked immunosorbent assay.Results:The expression of HSP70 protein in the peripheral serum of the experimental groups was higher than that in the normal control group.The peak time in both the model and the sham-operated groups was 12 h,and the peak time in the acupuncture group was 24 h.The expression in the acupuncture group declined to a lower level at 72 h and was lower than that in the model and sham-operated groups(P0.05).The peak time for the expression of TNF-αprotein in the peripheral serum of both the model and the acupuncture groups was 24 h,but the expression in the acupuncture group was lower than the model group.Additionally,the expression of TNF-αin all experimental groups was higher than the normal group(P0.05).Conclusions:Acupuncture at DU20 and ST36 in rats attenuated CIRI,which was associated with a reduction in the expression of HSP70 and TNF-α.These results provide clues to acupunctural neuroprotective properties.Acupuncture at DU20 and ST36 in rats after CIRI can adjust the expression of HSP70 and TNF-αin the peripheral serum,which might be one of the mechanisms of acupuncture's attenuation of CIRI.     

针刺对脑缺血再灌注损伤大鼠外周血清IL-1β、IL-6表达的影响

目的:探讨针刺百会和足三里穴对脑缺血再灌注损伤大鼠外周血清不同时间点白细胞介素1β(IL-1β)、白细胞介素6(IL-6)表达的干预作用。方法:将SD大鼠随机分为假手术组、模型组和针刺组3个大组,每大组再按再灌注后时间分为12 h、24 h、48 h、72 h、96 h和144 h等6个亚组,每亚组8只大鼠;另设正常对照组8只。以大脑中动脉线栓法制备大鼠脑缺血再灌注模型,用酶联免疫吸附测定法测各组大鼠外周血清IL-1β、IL-6的表达。结果:IL-1β在针刺组表达强度低于模型组,以24 h组和96 h组最显著(P<0.05);IL-6在针刺组的表达低于模型组和假手术组,其中24 h和48 h组具有显著性差异(P<0.05)。结论:针刺百会和足三里穴可抑制脑缺血损伤早期炎症反应,延缓组织损伤。     

Effects of Acupuncture at Baihui(GV 20) and Zusanli(ST 36) on Peripheral Serum Expression of MicroRNA 124,Laminin and Integrin β1 in Rats with Cerebral Ischemia Reperfusion Injury

Objective:To explore the effects of acupuncture at Baihui(GV 20) and Zusanli(ST 36) on the peripheral serum expression of microRNA 124(miRNA 124),laminin and integrin β 1 in rats with cerebral ischemia reperfusion injury(CIRI).Methods:Seventy-two healthy male Sprague-Dawley rats were randomized into a model group,an acupuncture group,and a sham-operated group using a random digits table,with 24 rats per group.Each group was further randomly divided into 1-,3-,5-,and 7-day subgroups based on the reperfusion time according to a random digits table,with 6 rats in each subgroup.In the model and acupuncture groups,CIRI was induced using the thread occlusion method.Electroacupuncture stimulation was applied daily to GV 20 and left ST 36 for 20 min at the indicated time points after successful operations.Serum was sampled for detecting laminin and integrin β1 protein via enzyme-linked immunosorbent assay,and serum miRNA 124 was examined using quantitative polymerase chain reaction.Results:The serum level of miRNA 124 in the cerebral ischemia rats increased significantly,and the peak expression of miRNA 124 in both the model and acupuncture groups occurred at 3 days.The expression of miRNA 124 in the acupuncture group was higher than in the model group at the same time point(5.96 ±0.01 vs.3.11 ±0.04,P0.05).Laminin expression in serum from the cerebral ischemia group was higher than that in the sham-operated group.Compared with the model group,the level of laminin in the serum of the acupuncture group was significantly lower at each time point,especially at the 3-day,and 7-day time points(589.12±3.57 vs.793.05 ± 5.28,and 600.53 ±3.05 vs.899.06 ±5.74,P0.05).The level of integrin β 1 in the serum from the acupuncture group was lower than that in the model group particularly at the 3-day and 7-day time points(208.66 ±0.95 vs.280.83 ±1.77,and212.36 ±0.95 vs.316.77±2.42,P0.05).Additionally,the model group and the acupuncture group showed dual peaks of integrin β 1 and laminin expression at 3-day and 7-day.Conclusions:Acupuncture at GV 20 and ST 36 in rats alleviated CIRI and was associated with upregulated expression of miRNA 124 and with downregulated expression of integrin β 1 and laminin in peripheral serum.These changes may represent one of the mechanisms underlying acupuncture's attenuation of CIRI.