小儿腹泻推拿治疗研究进展

小儿腹泻是儿科常见病．仅次于呼吸道感染．在国内外皆呈现高发病率。如克利夫兰的一项研究报告显示儿童每人每年发病1．52次，中国每年为1．76冼，人．农村高于城市．估计中国5岁以下儿童每年有3—4亿人次腹泻。其病原有细菌、病毒、原虫等，以病毒为多见．尤其是轮状病毒。其中夏季多发细菌性腹泻．秋冬季节则多发轮状病毒肠炎．据统计中国秋冬小儿腹泻中有半数为轮状病毒所致．可见轮状病毒是中国小儿腹泻的主要原因．与外国的报道一致。轮状病毒感染以6月～2岁婴幼儿为多见（约占93．5％），2岁以后小儿感染轮状病毒逐渐减少。至于其它病毒，尤其是肠道腺病毒。在小儿腹泻中的病原地位。墨尔本调查占6％，中国是小于5％。同样。诺瓦克病毒、星状病毒、杯状病毒、冠状病毒、小圆病毒也能引起腹泻。     

“Four-Step Massage” in treating childhood spleen-deficiency diarrhea

Objective To observe the clinical effects of a modified Tuina method in the treatment of childhood diarrhea. Methods Thirty children with diarrhea of spleen deficiency were treated by “Four-Step Tuina” (treatment group); and control group was treated with routine Tuina methods. The scale of children diarrhea was used as an objective index for therapeutic effect evaluation. Results After treatment, the scale was 1.4±1.92 in treatment group and 3.3±3.45 in control group; the statistical analysis showed that there was a difference between the scales; the treatment group had better effects than control group, in another word, the “Four-Step Tuina” was quite effective for children diarrhea. Conclusion Tuina has positive effects on children diarrhea of spleen deficiency, which provides evidences for the standardization of clinical therapeutic effects.      

Sau-PCR法分析由洋葱伯克霍尔德菌引起的院内感染

目的 通过使用Sau-PCR方法分析一起由洋葱伯克霍尔德菌引起的院内感染,为追溯感染源和感染途径提供有效的信息.方法 Sau-PCR首先使用限制性内切酶Sau3AI对基因组DNA进行酶切,获得以GATC序列为黏性末端的酶切产物,然后使用能够识别酶切位点(GATC)的引物对酶切产物进行PCR扩增,最后根据DNA琼脂糖凝胶电泳图谱分析样本之间同源性.结果 11株来自患者的菌株中,除1株以外,其余均表现出100%同源性,采用脉冲场凝胶电泳(PFGE)对样本进行验证,结果与Sau-PCR完全一致.结论 Sau-PCR是一种实用、简便、迅速的DNA指纹图谱分析工具,在对同源性的研究当中具有广阔应用前景.     

"Four-Step Massage" in Treating Childhood Spleen-Deficiency Diarrhea

目的:观察一种改良的推拿方法治疗小儿腹泻的疗效.方法:究采用随机对照,单盲方法,通过对"四步推拿法"治疗30例脾虚型腹泻患儿的临床观察,并设立对照组,将小儿腹泻症状分级量化评分作为疗效评价的客观指标.结果:治疗组治疗后症状量化总评分为1.4±1.92,对照组治疗后症状量化总评分为3.3±3.45,经统计学处理,两者有明显差异,治疗组优于对照组,说明四步推拿法疗效更佳.结论:推拿治疗小儿脾虚型腹泻的疗效确切.为进一步规范推拿临床诊疗评价体系提供了依据.