骨髓间充质干细胞对创伤性骨折愈合的促进作用

BACKGROUND:In recent years, the development of stem cell culture and isolation technologies provides new therapeutic choices for fracture healing. OBJECTIVE:To investigate the effect of exogenous bone marrow mesenchymal stem cells on bone fracture healing in traumatic fracture rats and on the migration ability of endogenous bone marrow mesenchymal stem cells. METHODS:Femoral fracture models were made in 48 Wistar rats and then randomized into experimental group and control group (n=24/group). Bone marrow mesenchymal stem cells from another healthy rats were isolated using adherent method and then injected into the rats via the tail vein in the experimental group. Rats in the control group were given the same volume of normal saline. At 2, 3, 4, 8, 12 weeks after injection, we extracted bone marrow mesenchymal stem cells from the femur of rats in the two groups. RT-qPCR was used to detect expression levels of type I collagen and CD44. Transwell method was used to detect cell migration ability. Immunohistochemitry method was employed to detect expression of nerve growth factors in the callus. RESULTS AND CONCLUSION:mRNA levels of type I collagen and CD44 in rat bone marrow mesenchymal stem cells were significantly higher in the experimental group than the control group at 2, 3 and 4 weeks after injection (P < 0.05). Compared with the control group, the higher migration ability of bone marrow mesenchymal stem cells was found in the experimental group at 2 and 3 weeks after injection (P < 0.05) as well as the higher expression of nerve growth factor in the callus in the experimental group at 3, 4, 8, 12 weeks after injection. All these findings suggest that exogenous bone marrow mesenchymal stem cells can improve the migration ability of endogenous bone marrow mesenchymal stem cells and the expression of nerve growth factor in the callus in rats with femoral fracture, thereby promoting fracture healing in rats.     

数字三维影像测量模型在复杂长骨骨折修复中的应用：随机对照、6个月随访、临床试验方案

BACKGROUND: During internal fixation for complex long bone fractures, it is difficult to position the internal fixator completely to the contours of the bone surface, often resulting in unstable fixation and affecting the quality of the repair. Digital three-dimensional (3D) models were therefore established based on computed tomography (CT) scans that closely reflected the site and degree of fractures, thereby guiding precise positioning of the internal fixator. From a safety point of view, the digital 3D-derived model was created to ensure that the internal fixator could achieve better repairs of complex long bone fractures.     

平阴县医疗单位消毒情况调查

作者对全县２５０个医疗单位进行消毒情况调查，检测表明，灭菌医疗用品消毒合格率为８５．７５％￣１００％。普通病房空气含菌量合格率为２８．７５％￣６０．００％，紫外线灯强度达到标准的为４２．１１￣６２．５０％。     

蚊蝇抗药性调查及其克服抗药性措施的研究

化学杀虫剂在消灭害虫方面起了重要作用，但随着时间的推移和杀虫互作的深入，污染环境和产生抗药性的弊病暴露得越来越明显，越来越引起人们的关注。我县已开展消杀互作多年，为了解蚊蝇对杀虫剂的抗药性情况，筛选敏感杀虫药，制定预防和延缓抗性产生的措施，2005年6～10月对县城驻地蚊蝇抗药性进行了调查，现将调查结果报告如下.     

改良Watson-Jones入路微创治疗髋关节后脱位合并PipkinⅠ、Ⅱ型股骨头骨折

目的探讨改良Watson-Jones入路在髋关节后脱位合并PipkinI、II型股骨头骨折微创治疗中的应用。方法 7例髋关节后脱位合并PipkinI、II型股骨头骨折患者,PipkinI型1例、II型6例,全部采用改良Watson-Jones入路切开复位钛质空心螺钉内固定治疗。结果按照Harris评分：90-96分,平均92.5分。结论改良Watson-Jones入路是治疗髋关节后脱位合并PipkinI、II型股骨头骨折的理想手术入路。     

涤纶毡片重建喙锁韧带治疗陈旧性肩锁关节脱位

涤纶毡片重建喙锁韧带治疗陈旧性肩锁关节脱位慕小瑜官众李洪杨杰山郭启发谭欣林赵希唐薛茂堂我们１９９１年１月～１９９７年５月采用涤纶毡型心脏补片（简称涤纶毡片）重建喙锁韧带，治疗陈旧性肩锁关节脱位１３例，疗效满意，现报告如下。１．临床资料：本组陈旧性肩关...     

腓肠神经营养血管逆行岛状皮瓣修复小腿下软组织缺损

1临床资料1.1一般资料2004~2005年我院收治小腿下1/3皮肤缺损患者11例,其中男性9例,女性2例。年龄34~75(43.2)岁。缺损部位:1例陈旧性足跟皮肤缺损,7例急性踝部皮肤挫灭伤,小腿前方缺损患者2例,前足部截肢术后1例。皮瓣面积为8 cm×10 cm~10 cm×18 cm。11例皮瓣全部成活,2例皮     

对基层公共卫生体制改革的思考

公共卫生体制改革是医疗卫生体制改革的重要组成部分，从大头娃娃、苏丹红、吊白块、三聚氰胺等食品安全事件到SARS、禽流感、手足口等疫情的发生，给我们国家带来了前所未有的灾难，也给我们敲响了警钟，同时也暴露出疾病预防控制体系和卫生监督体系存在的致命弱点，因此改革建立符合我国国情的疾病预防控制体系和卫生监督体系，势在必行。本文通过对目前公共卫生体制现状及存在的主要问题的分析，提出公共卫生体制改革的建议。     

骨水泥型股骨柄人工股骨头置换治疗老年骨质疏松股骨颈骨折的可行性：随机对照临床试验方案

BACKGROUND: Osteogenesis is a common problem after surgery for femoral neck fracture in elderly patients. Internal fixation for the treatment of femoral neck fracture should be performed to optimize bone remodeling and strengthen fractured bone trabeculae, with the aim of achieving strong fixation from the perspective of biomechanics. Percutaneous internal fixation with cannulated compression screws has become a preferred treatment method of osteoporotic femoral neck fracture in elderly patients, but the insufficient holding power of the screws used in the femoral neck does not lead to strong fixation. An alternative, joint prosthesis, is recommended for the repair of femoral neck fracture in elderly patients. However, its long-term therapeutic effects in the treatment of osteoporotic fracture of proximal femoral neck remain poorly understood in patients with avascular necrosis of the femoral head.     

膝关节炎软骨基质金属蛋白酶2、基质金属蛋白酶9表达与MRI表现的相关性

BACKGROUND: Matrix metalloproteinases (MMPs) have been shown to break the balance between the production and degradation of extracellular matrix by degrading collagen, gelatin, elastin and other macromolecules, which is the leading cause of bone and cartilage damage in rheumatic arthritis. OBJECTIVE: To determine the expression levels of MMP-2 and MM-9 in the rabbit knee osteoarthritis cartilage, and to analyze whether they are related to the early MRI performance. METHODS: Fifty healthy Japanese white rabbits free from osteoarthritis were enrolled and randomly divided into control group (n=10) and model group (n=40). The anterior and posterior cruciate ligament amputation was adopted to establish the model of osteoarthritis. Subsequently, MRI examination and the expression levels of MMP-2 and MMP-9 were detected at 1, 3, 5 and 7 weeks (n=10 at each time point), respectively. RESULTS AND CONCLUSION: MRI examination showed that more damaged cartilage appeared with time in the rabbit model of osteoarthritis, and significantly different from MRI classification (P < 0.05). The expression levels of MMP-2 and MMP-9 mRNA in the model group were always significantly higher than those in the control group (P < 0.05); MMP-2 level reached the highest at 3 weeks after modeling (P < 0.01), and then presented a slight decline; MMP-9 level peaked at 5 weeks after modeling (P < 0.01) and slightly decreased at 7 weeks. MRI classification of cartage injury and expressions of MMP-2 (R2=0.119, P=0.119) and MMP-9 (R2= 0.466, P=0.466) were shown to have the correlation. These results illustrate that the serum levels of MMP-2 and MMP-9 are related to the early destruction of articular cartilage in patients with osteoarthritis, and can be used as predictors for early osteoarthritis. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程