重用赤芍治疗ANIT诱导大鼠急性淤胆型肝炎的研究

目的:观察不同剂量赤芍对α-萘异硫氰酸酯(α-naphthylisothiocy,ANIT)诱导大鼠急性淤胆型肝炎的保护作用.方法:赤芍高、中、低(分别相当于原生药量36,18,1g·kg-1)剂量ig给药3 d(2次/d)后,采用ANIT(60 mg·kg-1)诱导大鼠急性淤胆型肝炎模型,造模后继续给药3次,末次给药1h后,进行胆管引流,记录大鼠6h内的胆汁流量,检测血清学指标变化及肝脏病理学改变,结合对应分析综合评价赤芍对ANIT诱导大鼠急性淤胆型肝炎的影响.结果:血清生化结果显示,与模型组相比,赤芍中、高剂量给药组血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、碱性磷酸酶(ALP)、总胆汁酸( TBA)明显降低(P＜0.01),胆汁流量显著升高(P＜0.05),低剂量组变化不明显;病理组织学观察结果显示,与模型组相比,赤芍中、高剂量组肝脏病变均有明显改善,低剂量组改善不明显;对应分析结果表明,赤芍中、高剂量组的治疗效果显著,且呈剂量依赖性增强.结论:高、中剂量赤芍(36,18 g·kg-1)对ANIT诱导的大鼠急性淤胆型肝炎有显著的保护作用.     

高压氧治疗原发性三叉神经痛的临床研究

目的:探究原发性三叉神经痛应用高压氧(HBO)治疗的临床效果。方法:选取2017年1-7月本院收治的原发性三叉神经痛患者60例。按照随机数字表法将其分为对照组与研究组,各30例。对照组给予卡马西平口服治疗,研究组在对照组基础上增加HBO治疗。比较两组的短期疗效[治疗期间不良反应、治疗结束后的视觉模拟疼痛评分(VAS)、治疗效果]、治疗前后不同时间的VAS评分、治疗后不同时间点对治疗的总体感觉、治疗前后卡马西平的口服剂量。结果:研究组治疗结束后VAS评分低于对照组(P0.05),临床总有效率高于对照组(P0.05),两组治疗期间不良反应发生率比较差异无统计学意义(P0.05);治疗后1、2、3、6个月,研究组VAS评分均低于对照组(P0.05);治疗结束时及治疗后6个月,研究组治疗感觉有效率为76.67%、80.00%,均高于对照组的43.33%、36.67%,比较差异均有统计学意义(P0.05);治疗后1、2、6个月,研究组口服药物剂量均小于对照组,比较差异均有统计学意义(P0.05)。结论:HBO治疗原发性三叉神经痛可改善患者疼痛、提升临床效果、改善患者对治疗的总体感觉。     

苷泰胶囊抗乙肝病毒的实验研究

目的观察苷泰胶囊对乙肝病毒复制的抑制作用。方法使用鸭乙肝炎病毒使1日龄的北京鸭感染,造模7 d后给药(ig),使用斑点杂交法检验用药后(5、10 d)及停药5 d后血清中的DHBV DNA含量;以转染的2.2.15细胞为模型,分别加入不同浓度的苷泰胶囊药液,分别以酶联免疫法及斑点杂交法检测培养上清液中的HbsAg,HbeAg及HBV DNA的水平。结果 (1)用药后10 d和停药后3 d鸭血清中的DHBV DNA水平显著低于给药前(P<0.01,P<0.05);(2)苷泰胶囊可以明显地抑制2.2.15细胞HbsAg、HbeAg的分泌(P<0.01,P<0.05),其半数抑制浓度分别为1 158.92μg/mL和1 794.14μg/mL;(3)苷泰胶囊明显地抑制2.2.15细胞HBV-DNA的复制,其半数抑制浓度为701.27μg/mL。结论实验结果表面苷泰胶囊对2.2.15细胞及鸭乙肝病毒感染的模型均具有一定抑制作用并呈剂量依赖性。     

Research on Fuzi based on animal thermotropism behavior to discover if it has fewer "hot" characteristics without Ganjiang

OBJECTIVE:To investigate whether Fuzi(Radix Aconiti Praeparata) has fewer "hot" characteristics when administered without Ganjiang(Rhizoma Zingiberis).METHODS:Differences in the thermotropism behaviors of mice treated either with Fuzi(Radix Aconiti Praeparata),Ganjiang(Rhizoma Zingiberis) or the combination of the two given intragastrically were investigated using the Animal Thermotropism Behavior Surveillance System.The water intake volume,oxygen consumption volume,adenosine triphosphatase(ATPase) activity,total antioxidant capacity(T-AOC) and total superoxide dismutase(T-SOD) activity were determined during the investigation.RESULTS:When Fuzi and Ganjiang were administered together,the rate at which mice remained on a warm plate("remaining rate") and the times and distances of their movement were all significantly reduced(P<0.05).Compared with the Normal group,the reduction was 55.1%,48.3% and 44.8%,while compared with the Fuzi group,the reduction was 57.6%,34.3% and 36.0%,indicating that "cold" tropism was significantly increased.Compared with the Normal and Fuzi groups,the ATPase activity and the respiratory oxygen consumption volume of the Fuzi + Ganjiang group were significantly increased(P<0.05),suggesting an improvement in energy metabolism and showing a "hot" characteristic when Fuzi and Ganjiang are present together.Additionally,the T-AOC and T-SOD activity were significantly enhanced(P<0.05).CONCLUSION:The behavior of mice tending toward "cold" tropism can be regarded as a quantitative reflection of Fuzi having fewer characteristics consistent w ith a "hot" nature when not used with Ganjiang,the functional mechanism of which may be a change in the ATPase activity in liver tissue.