Effects of electroacupuncture of different frequencies on SP and VIP expression levels in colon of rats with slow transit constipation

Objective

To observe the effects of electroacupuncture (EA) of different frequencies on the expression levels of substance P (SP) and vasoactive intestinal peptide (VIP) in the colon of rats with slow transit constipation (STC).

Methods

One hundred healthy male Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group, a low-frequency EA group, a high-frequency EA group and a variable-frequency EA group, with 20 rats in each group. The rats in the normal group were fed with normal diet. The rats in the other groups were fed with phenethylpiperidine in the diet at a dose of 8 mg/(kg·bw) per day, for 120 d, to establish the STC model. Rats in the normal group and the model group did not receive any treatment; rats in the low-frequency EA group were treated with 2 Hz continuous wave EA, rats in the high-frequency EA group were treated with 100 Hz continuous wave EA, and rats in the variable-frequency EA group were treated with 2 Hz/100 Hz sparse-dense EA. The current intensity of the EA was determined by the slight vibration of the rat limbs without painful screaming. The intervention was performed once a day, 15 min/time for continuous 15 d. After treatment, the intestinal transit function and the expression levels of SP and VIP in the colon of the rats in each group were determined.

Results

After treatment, the defecation duration of the first dark stool in the model group was significantly longer than that in the normal group (P<0.05); the defecation durations of the first dark stool in the low-frequency EA group, high-frequency EA group and variable-frequency EA group were significantly shorter than the duration in the model group (all P<0.05); compared with the low-frequency EA group, the first dark stool defecation duration of rats in the variable-frequency EA group was significantly shorter (P<0.05); compared with the normal group, the SP and VIP expression levels in the colon of the model group were significantly decreased (both P<0.01); the SP and VIP expression levels in the colon of the low-frequency EA group, the high-frequency EA group and the variable-frequency EA group were significantly higher than those in the model group (all P<0.05); compared with the high-frequency EA group, the SP expression levels in the colon in the low-frequency EA group and the variable-frequency EA group were significantly increased (both P<0.05); compared with the low-frequency EA group, the VIP expression levels in the colon in the high-frequency EA group and the variable-frequency EA group were significantly increased (both P<0.05).

Conclusion

EA improves the intestinal function of STC model rats by regulating the expression levels of SP and VIP in rat colon. The EA stimulation with 100 Hz continuous wave, 2 Hz/100 Hz sparse-dense wave shows a better improvement in the colonic transit function in STC rats, followed by 2 Hz continuous wave.

     

芒针温针灸对慢传输型便秘肠道传输功能的影响

目的观察芒针温针灸对慢传输型便秘(STC)的治疗效果。方法将209例STC患者随机分为治疗组(106例)和对照组(103例),治疗组采用芒针温针灸治疗,对照组采用西沙必利口服,分别对两组治疗效果和结肠传输功能进行疗效评估。结果经治疗后,治疗组总有效率为92.45%,明显高于组总有效率(79.61%,P0.05)。与治疗前比较,两组均可加速肠道内标记物清除速度(P0.01),治疗组肠道内标记物的清除速度更快(P0.01)。结论芒针温针灸治疗可明显改善STC患者结肠传输功能。     

经阴道子宫输卵管声诺维三维超声造影诊断输卵管性不孕症的初步探讨——附36例检测报告

目的：探讨经阴道子宫输卵管声诺维三维超声造影（3D-SonoVue-HyCoSy）诊断输卵管性不孕症的可行性及诊断效能。方法：选择36例不孕症患者共72条输卵管,利用经阴道子宫输卵管3D-SonoVue-HyCoSy诊断输卵管的通畅性和盆腔黏连情况,以腹腔镜下通液检查结果为标准,评价3D-SonoVue-HyCoSy对输卵管通畅性的诊断效能。结果：3D-SonoVue-HyCoSy诊断输卵管阻塞的阳性预测值为87%,诊断输卵管通畅的阴性预测值为84%,诊断通而不畅的符合率为20%,判断输卵管通畅与否的准确度是76%,诊断输卵管阻塞的敏感度为84%,诊断输卵管通畅的特异度为75%。在通畅及通而不畅的35条输卵管的3D-SonoVue-HyCoSy图像中,83%输卵管可见伞端溢出,通畅的25条输卵管中88%可见同侧卵巢周边造影剂溢出环,10条通而不畅的输卵管中7条可见同侧卵巢周边少量溢出;检出盆腔黏连的敏感度15%。结论：经阴道子宫输卵管3D-SonoVue-HyCoSy诊断输卵管性不孕症是可行和有效的,自动三维容积成像功能和声诺维超声造影剂的使用明显提高了输卵管内造影剂持续流动信号的探测水平和输卵管伞端造影剂溢出的识别水平。     

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三维子宫输卵管超声造影检查和图像分析

目的初步探讨三维子宫输卵管造影对输卵管通畅性的诊断价值。方法回顾分析2008年4月—2010年12月在本院就诊并自愿接受三维子宫输卵管超声造影检查的不孕症患者197例。结果通畅、通而不畅和阻塞的输卵管中,伞端明显可见率依次为89.0%、30.3%、4.0%;卵巢周边明显光环显示率分别为90.0%、52.3%、17.3%;将197例患者按照输卵管通阻情况分为6组,各组子宫直肠窝造影剂明显积聚发生率从高到低依次为97.1%、82.7%、68.2%、40.0%、17.6%、0.0%;有明显阻力发生率从高到低依次为72.2%、35.3%、30.0%、27.3%、3.8%、2.9%;有明显不适发生率各组间差异有统计学意义。结论综合分析三维和编码对比显像模式下输卵管伞端、卵巢周边明显光环显示率等可用于评估输卵管通畅性,三维子宫输卵管造影动态、简单、无创,可作为检测输卵管通畅性的重要方法。     

冷水作业工人甲皱微循环检查

冷水作业工人甲皱微循环检查马德元张莘我们在９０年代初期，对天津市某单位直接从事冷水作业的工人进行了甲皱微循环检查，为判断冷水对手部的损伤程度及为冷水作业工人的劳动保护措施提供科学依据。一、对象与方法１．对象：实验组为随机选取徒手冷水作业（０℃）、排除...     

131Ⅰ-抗-c-erbB-2单抗对卵巢癌细胞SKOV3的抑制作用

目的观察^131Ⅰ标记针对癌蛋白p185为靶点的单克隆抗体在体外对卵巢癌细胞的抑制作用。方法用氯胺T法^131Ⅰ标记抗-c-erbB-2单克隆抗体(anti-c-erbB-2-McAb)，取不同浓度的^131Ⅰ-anti-c-erbB-2-McAb与SKOV3细胞共同培养，以流式细胞术检测其对SKOV3细胞生长的抑制作用。结果^131Ⅰ-anti-c-erbB-2-McAb在体外可明显抑制SKOV3细胞，^131Ⅰ-rmIgG的抑制作用较弱，未标记的anti-c-erbB-2-McAb对SKOV3细胞的生长几乎没有抑制作用：结论^131Ⅰ-anti-c-erbB-2-McAb能有效抑制肿瘤细胞生长，有可能用于卵巢癌的免疫导向治疗。     

长时间放置cook球囊支架预防宫腔粘连术后复发的临床应用

目的探讨长时间放置cook球囊支架预防宫腔粘连(IUA)术后复发的有效性及安全性。方法 134例中、重度IUA患者,行宫腔镜下宫腔粘连分离术(TCRA),术后宫腔放置cook球囊2~3个月预防宫腔粘连术后复发,随访效果。结果所有患者均无支架嵌顿;手术前后宫腔粘连美国生育协会(AFS)预后评分分别为(8.01±1.33)、(0.45±0.75)分,差异有统计学意义(P<0.05)。手术前后AFS月经模式评分分别为(1.61±1.23)、(0.69±0.97)分,差异有统计学意义(P<0.01)。患者累积妊娠率为60.48%,累积活产率为20.90%。放置支架所需时间为(7.3±5.1)min;1例放置支架扩张宫颈时导致子宫穿孔;4例支架放置失败;4例因症中途取出支架;5例术后经间期少量出血,2例术后出血≥500 ml;1例发生术后急性生殖道感染;15例在未达规定时间支架下移或脱出;带器妊娠1例。结论宫腔粘连分离术后宫腔内长时间放置cook球囊在重建宫腔形态、预防术后再粘连、改善月经模式及远期生育结局方面有较明显的成效。     

近年热敏灸疗法的研究概况

通过收集2010年6月－2015年3月近5年内关于热敏灸疗法的临床、实验研究文献,结合刺灸法数据挖掘课题组所采集热敏灸在各科的数据信息,筛选出在5个科属中具有代表性的病种及临床研究试验进行了归纳。     

原发性气管癌1例

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