元胡的器官分化和生物碱形成的关系

 本文讨论了元胡愈伤组织的分化与形成生物城的关系。元胡愈伤组织和细胞培养物（悬浮培养）大多丧失了合成生物碱的能力，而分化出的再生小球茎，无论在固体或液体悬浮培养条件下都 能正常生长，恢复了生物碱合成能力，能形成镇痛的主要成分延胡索乙素，总生物碱的含量也达到了 植物样品（球茎）的水平。.这一现象对于药用植物組织培养法应用于生产具有一定的实践意义。     

丹参毛状根培养的建立和丹参酮的产生

丹参毛状根培养的建立和丹参酮的产生张荫麟,宋经元,吕桂兰,刘惠灵(中国医学科学院药用植物研究所北京100094)丹参SalviamiltiorrhizaBunge是治疗心血管系统疾病的重要中革药,主要成分如丹参酮具有活血、抗菌等活性,同时也是一种红色色素。利用细胞培养方法来生产这些活性成分,不仅对满足临床用药需要有重要意义,作为一种具有生理活性的色素开发也有良好前景。植物毛状根培养是80年代发展起来的植物基因工程和细胞工程相结合的?...     

野罂粟悬浮细胞培养及其野罂粟生物碱的产生

对野罂粟细胞悬浮培养及其次生代谢物野罂粟生物碱进行了研究。结果显示，在培养的中后期补充营养物质可促进细胞生长及生物碱的累积。3％的蔗糖浓度对细胞的生长最为有利；4％的蔗糖对总生物碱的累积最为适宜。悬浮细胞在pH5．7的培养基中生长最为旺盛，而在pH5．4的培养基中总生物碱的含量最高。取IC法测定野罂粟悬浮培养物中野罂粟碱的含量为0．0235mg/g DW，黑龙辛甲醚的含量为0．022mg/g DW。     

丹参冠瘿组织的生长和总丹参酮的积累动态

AIM　To determine the dynamics of growth and total tanshinones accumulation in crown gall cultures of Salvia miltiorrhiza in MS and 67-V liquid media. METHODS　Fresh, dry weight and total tanshinones yields in the cultures and in the medium were determined every 5 days in crown gall suspension cultures. RESULTS　In MS medium, the logarithmic growth phase of crown gall cultures in S.miltiorrhiza was from the 5th to 30th days, and the stationary growth phase was from the 30th to 35th days. From the 25th to 30th days, physiological activity of crown gall cultures was higher and their growth was better. However, in 67-V medium, the logarithmic growth phase of crown gall cultures was from the 10th to 25th days, and the stationary growth phase was from the 25th to 35th days. Total tanshinones were largely accumulated in the cultures and in the medium after 25 days. The total tanshinones yield (60 mg.L^-1) was reached at the 35th day. CONCLUSION　Knowing the regularity of the growth and total tanshinones accumulation in crown gall cultures of S.miltiorrhiza will be helpful to take proper regulative measures in order to obtain the maximum total tanshinones yield.     

迈向二十一世纪的药用植物生物技术

本文阐述了当今涉及到基因工程，细胞工程，组织、器官培养等几个层次上所进行的药用植物生物技术。同时对细胞培养与活性成分生产，转基因器官培养，转基植物，药用植物有效成分的调控技术等当前药用植物生物技术的研究热点进行了讨论。     

灵芝担孢子散发规律的观察

本文研究了北京地区栽培灵芝在5至11月份的产孢子粉动态以及单株灵芝的产粉情况,试验了在培养基中添加营养物质来提高产粉量的方法。