贻贝多糖的工业化制备工艺研究

目的 建立适于工业放大的贻贝多糖提取工艺。方法 以厚壳贻贝为对象,对贻贝多糖制备过程中原料筛选、浸提条件、除蛋白质、醇沉等技术环节进行改进和优化。最后利用改进的工艺进行了贻贝多糖的中试制备。结果 厚壳贻贝多糖为白色粉末,多糖平均得率为贻贝干重的14.1%。MP-A为厚壳贻贝多糖的主要成分,占比约为89.42%,相对分子质量为1,268.15 kD。结论 建立了流程简单、绿色环保、易于放大的贻贝多糖工业化制备工艺,能获得纯度90%左右的贻贝多糖样品。     

玻璃酸钠及其在外科应用的研究进展

玻璃酸钠作为高分子多糖,对骨关节疾病有良好的治疗作用,近年来对其衍生物的研究越来越广泛.我们综述了玻璃酸钠及其衍生物在治疗骨关节疾病、防粘连、组织工程方面以及治疗软骨缺陷等方面的应用情况和发展前景.     

不同浓度木瓜蛋白酶建立兔膝骨关节炎模型的比较研究

目的:比较不同浓度木瓜蛋白酶建立的兔膝骨关节炎模型,为研究骨关节炎病理及诊疗方法提供数据支持。方法:将60只新西兰大耳白兔随机分为4组,分别在1、3、5d右膝关节腔注射2%(低剂量组)、5%(中剂量组)、10%(高剂量组)(w/v)木瓜蛋白酶和0.03mol/L的L-半胱氨酸混合溶液0.1ml/kg(实验组),以及等量生理盐水(对照组)。整个实验过程观察兔状态并记录体重和膝关节宽度。首次注射后第2、4、6周将兔分批处死,取股骨髁、胫骨平台及滑膜做大体及组织病理学观察,并进行评分。结果:实验组均出现关节宽度增大、关节软骨变薄、基质分解破坏、滑膜增生等炎症及退行性改变,严重程度随浓度增高而增高,也随时间延长而发展;对照组无上述改变,二者比较差异有统计学意义。结论:兔膝骨关节腔注射2%、5%、10%木瓜蛋白酶和0.03mol/LL-半胱氨酸混合溶液0.1ml/kg可制备不同严重程度的骨关节炎模型,周期短,可重复且成功率高。     

透明质酸钠在关节疾病中的应用

目的：综述透明质酸钠在关节内的生理功能以及在关节疾病中的临床应用。方法：广泛查阅相关文献资料，分析透明质酸钠治疗关节疾病的机制及应用前景。结果：外源性透明质酸钠通过参与改善关节滑液、保护关节软骨、抑制炎症发生等，在膝骨关节炎、类风湿性关节炎、颞下颌关节炎等疾病中均可发挥作用。结论：透明质酸钠对关节疾病具有很好的治疗作用，应用前景广阔。     

Clinical observations on the therapeutic effect of compound sinomenine hydrochloride and sodium hyaluronate sustained-release injection in treatment of rabbit osteoarthritis synovial disease

Objective: To discuss the sustained-release effect of compound sinomenine hydrochloride and sodium hyaluronate sustained-release injection (CSSSI) on sinomenine hydrochloride and its therapeutic effect on rabbit osteoarthritis synovial disease after injected into the articular cavity. Methods: Compound injection consists of 1% sodium hyaluronate and 2.5% sinomenine hydrochloride was prepared as an experimental group, and 2.5% sinomenine hydrochloride was prepared as a control group. The cumulative mass release was measured at different time points for each group in vitro. 65 male Zelanian rabbits were randomly divided into five groups: 15 (30 knees) for the control, sodium hyaluronate, sinomenine hydrochloride and CSSSI groups, and five (10 knees) for the modeling group. Papain articular cavity was modeled with both knees of rabbits. Seven days later the rabbits of the modeling group were sacrificed, thus the modeling succeeded. Then, 0.2 ml corresponding drugs were injected into articular cavities of the remaining experimental groups, while the control group was treated with 0.2 ml normal saline, once a week for 4 weeks. Seven days after the last treatment, the rabbits were sacrificed by air injection into the auricular vein, and anatomized at knees to perform pathological observations and Mankin’s evaluation of synovium. Four groups were better evaluated, and a=0.05 was used as cutoff for variance analysis; LSD tests were carried out on every two groups; the SPSS13.0 software package was used. Results: In the in vitro sustained-release experiments, 90% of drugs was released in the control group of sinomenine hydrochloride sustained-release injection after 360 minutes. Comparisons of Mankin’s evaluations of four groups, P<0.05, indicates the statistical discrepancies in osteoarthritis therapy among the four groups. In further pairwise comparisons of CSSSI group vs. modeling control/sodium hyaluronate/sinomenine hydrochloride groups, P<0.05 was always obtained. Moreover, P<0.05 was for sodium hyaluronate vs. modeling control and sinomenine hydrochloride vs. modeling control, whereas P>0.05 for sodium hyaluronate vs. sinomenine hydrochloride. Conclusion: CSSSI has sustained-release effect on sinomenine hydrochloride. Articular cavity injection of CSSSI treats the rabbit osteoarthritis synovial disease better than sole sodium hyaluronate or sinomenine hydrochloride.     

羊眼玻璃体透明质酸钠的制备与分析

用羊眼制备的透明质酸钠,经光谱测定和理化分析其质量标准达到眼科用透明质酸钠的要求。     

走自主创新道路推动科技成果产业化

山东省生物药物研究院是省属开发型研究单位，其前身为山东省商业科技研究所及山东省生物药物研究所。主要从事天然药物、生化药物、生物技术、化学药物、药用原料和保健食品、化妆品的研究开发和生产销售。     

缩瞳药氨甲酰胆碱的合成及药效实验

采用一锅两步连续反应法合成了氨甲酰胆碱，其化学结构经红外光谱、核磁共振谱和元素分析证实。向兔眼前房注射０．０１％氨甲酰胆碱０．３ｍｌ，１～２ｓ瞳孔即开始缩小，５ｍｉｎ后瞳孔由１１．９ｍｍ缩小为２．３ｍｍ。     

玻璃酸钠对关节腔内注入木瓜蛋白酶所致兔膝关节退行性改…

采用在兔膝关节内注入木瓜蛋白酶造成的膝关节退行性改变模型，考察关节腔内长期多次注射玻璃酸钠对这种退行性改变的抑制和改善作用。结果表明：玻璃酸钠对已造成退行性改变的关节软骨有减轻和改善其病变程度的作用。     

透明质酸研究概况

<正> Meyer和Palmer1934年从牛眼玻璃体中分离出一种高粘性物质,命名为透明质酸(HA)。此名称是由Hyaloid(透明的、玻璃状的)与Uronic Acid(糖醛酸)复合而成的。五十年来,无论在HA分布和化学结构方面还是在其生理功能和临床应用方面的研究均取得了较大进展。各种经典分离技术的应用,加上近年来新技术的出现,不仅可以从多种组织中分离出HA,而且已有了商品生产。产品从生化试剂发展到了具有较高临床价值的药物。我国近年来也开始了药用HA制备和临床应用的研究,并取得了一定进展。本文对国内外HA