全文获取类型
收费全文 | 1962篇 |
免费 | 251篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 40篇 |
儿科学 | 114篇 |
妇产科学 | 67篇 |
基础医学 | 260篇 |
口腔科学 | 55篇 |
临床医学 | 282篇 |
内科学 | 444篇 |
皮肤病学 | 40篇 |
神经病学 | 121篇 |
特种医学 | 154篇 |
外科学 | 188篇 |
综合类 | 28篇 |
预防医学 | 147篇 |
眼科学 | 6篇 |
药学 | 133篇 |
中国医学 | 4篇 |
肿瘤学 | 136篇 |
出版年
2022年 | 7篇 |
2021年 | 20篇 |
2020年 | 12篇 |
2019年 | 31篇 |
2018年 | 48篇 |
2017年 | 16篇 |
2016年 | 27篇 |
2015年 | 32篇 |
2014年 | 48篇 |
2013年 | 73篇 |
2012年 | 102篇 |
2011年 | 79篇 |
2010年 | 78篇 |
2009年 | 70篇 |
2008年 | 97篇 |
2007年 | 92篇 |
2006年 | 95篇 |
2005年 | 101篇 |
2004年 | 72篇 |
2003年 | 82篇 |
2002年 | 63篇 |
2001年 | 76篇 |
2000年 | 84篇 |
1999年 | 78篇 |
1998年 | 47篇 |
1997年 | 55篇 |
1996年 | 56篇 |
1995年 | 42篇 |
1994年 | 38篇 |
1993年 | 33篇 |
1992年 | 45篇 |
1991年 | 25篇 |
1990年 | 25篇 |
1989年 | 51篇 |
1988年 | 39篇 |
1987年 | 44篇 |
1986年 | 34篇 |
1985年 | 33篇 |
1984年 | 15篇 |
1983年 | 12篇 |
1982年 | 9篇 |
1981年 | 20篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1978年 | 7篇 |
1977年 | 13篇 |
1976年 | 10篇 |
1974年 | 14篇 |
1973年 | 13篇 |
1971年 | 11篇 |
排序方式: 共有2219条查询结果,搜索用时 31 毫秒
1.
Olivier Chiche Josep Rods‐Cabau Francisco Campelo‐Parada Afonso B. Freitas‐Ferraz Ander Regueiro Chekrallah Chamandi Tania Rodriguez‐Gabella Mlanie Ct Robert DeLarochellire Jean‐Michel Paradis Eric Dumont Daniel Doyle Siamak Mohammadi Sbastien Bergeron Philippe Pibarot Jonathan Beaudoin 《Echocardiography (Mount Kisco, N.Y.)》2019,36(4):722-731
2.
Bianca Leclerc Sophie Bergeron Audrey Brassard Claude Bélanger Marc Steben Bernard Lambert 《Archives of sexual behavior》2015,44(6):1561-1572
Provoked vestibulodynia (PVD) is a prevalent women’s sexual pain disorder, which is associated with sexual function difficulties. Attachment theory has been used to understand adult sexual outcomes, providing a useful framework for examining sexual adaptation in couples confronted with PVD. Research to date indicates that anxious and avoidant attachment dimensions correlate with worse sexual outcomes in community and clinical samples. The present study examined the association between attachment, pain, sexual function, and sexual satisfaction in a sample of 101 couples in which the women presented with PVD. The actor–partner interdependence model was used in order to investigate both actor and partner effects. This study also examined the role of sexual assertiveness as a mediator of these associations via structural equation modeling. Women completed measures of pain intensity and both members of the couple completed measures of romantic attachment, sexual assertiveness, sexual function, and satisfaction. Results indicated that attachment dimensions did not predict pain intensity. Both anxious and avoidant attachment were associated with lower sexual satisfaction. Only attachment avoidance predicted lower sexual function in women. Partner effects indicated that higher sexual assertiveness in women predicted higher sexual satisfaction in men. Finally, women’s sexual assertiveness was found to be a significant mediator of the relationship between their attachment dimensions, sexual function, and satisfaction. Findings highlight the importance of examining how anxious and avoidant attachment may lead to difficulties in sexual assertiveness and to less satisfying sexual interactions in couples where women suffer from PVD. 相似文献
3.
Long‐term consequences of pain,anxiety and agitation for critically ill older patients after an intensive care unit stay 下载免费PDF全文
4.
Bergantini L Cameli P d’Alessandro M Vagaggini C Refini RM Landi C Pieroni MG Spalletti M Sestini P Bargagli E 《Clinical and experimental medicine》2019,19(4):487-494
Clinical and Experimental Medicine - Background The pathogenetic and regulatory roles of natural killer (NK) and natural killer T-like cells in interstitial lung diseases (ILDs), fibrotic and... 相似文献
5.
6.
Mohamed Magdy Ibrahim MD Jennifer Bond PhD Andrew Bergeron BA Kyle J. Miller BA Tosan Ehanire BA Carlos Quiles MD Elizabeth R. Lorden MS Manuel A. Medina MD Mark Fisher MD Bruce Klitzman PhD M. Angelica Selim MD Kam W. Leong PhD Howard Levinson MD 《Wound repair and regeneration》2014,22(6):755-764
Hypertrophic scar (HSc) contraction following burn injury causes contractures. Contractures are painful and disfiguring. Current therapies are marginally effective. To study pathogenesis and develop new therapies, a murine model is needed. We have created a validated immune‐competent murine HSc model. A third‐degree burn was created on dorsum of C57BL/6 mice. Three days postburn, tissue was excised and grafted with ear skin. Graft contraction was analyzed and tissue harvested on different time points. Outcomes were compared with human condition to validate the model. To confirm graft survival, green fluorescent protein (GFP) mice were used, and histologic analysis was performed to differentiate between ear and back skin. Role of panniculus carnosus in contraction was analyzed. Cellularity was assessed with 4′,6‐diamidino‐2‐phenylindole. Collagen maturation was assessed with Picro‐sirius red. Mast cells were stained with Toluidine blue. Macrophages were detected with F4/80 immune. Vascularity was assessed with CD31 immune. RNA for contractile proteins was detected by quantitative real‐time polymerase chain reaction (qRT‐PCR). Elastic moduli of skin and scar tissue were analyzed using a microstrain analyzer. Grafts contracted to ~45% of their original size by day 14 and maintained their size. Grafting of GFP mouse skin onto wild‐type mice, and analysis of dermal thickness and hair follicle density, confirmed graft survival. Interestingly, hair follicles disappeared after grafting and regenerated in ear skin configuration by day 30. Radiological analysis revealed that panniculus carnosus doesn't contribute to contraction. Microscopic analyses showed that grafts show increase in cellularity. Granulation tissue formed after day 3. Collagen analysis revealed increases in collagen maturation over time. CD31 stain revealed increased vascularity. Macrophages and mast cells were increased. qRT‐PCR showed up‐regulation of transforming growth factor beta, alpha smooth muscle actin, and rho‐associated protein kinase 2 in HSc. Tensile testing revealed that human skin and scar tissues are tougher than mouse skin and scar tissues. 相似文献
7.
Svetlana Grabauskiene Edward J. Bergeron Guoan Chen Dafydd G. Thomas Thomas J. Giordano David G. Beer Meredith A. Morgan Rishindra M. Reddy 《The Journal of surgical research》2014
Background
When presenting with advanced stage disease, lung cancer patients have <5% 5-y survival. The overexpression of checkpoint kinase 1 (CHK1) is associated with poorer outcomes and may contribute to therapy resistance. Targeting CHK1 with small-molecule inhibitors in p53 mutant tumors might improve the effectiveness of chemotherapy and radiotherapy in non–small cell lung cancer (NSCLC).Methods
We evaluated CHK1 messenger RNA and protein levels in multiple NSCLC cell lines. We assessed cell line sensitization to gemcitabine, pemetrexed, and radiotherapy by CHK1 inhibition with the small molecule AZD7762 using proliferation and clonogenic cell survival assays. We analyzed CHK1 signaling by Western blotting to confirm that AZD7762 inhibits CHK1.Results
We selected two p53 mutant NSCLC cell lines with either high (H1299) or low (H1993) CHK1 levels for further analysis. We found that AZD7762 sensitized both cell lines to gemcitabine, pemetrexed, and radiotherapy. Chemosensitization levels were greater, however, for the higher CHK1 protein expressing cell line, H1299, when compared with H1993. Furthermore, analysis of the CHK1 signaling pathway showed that H1299 cells have an increased dependence on the CHK1 pathway in response to chemotherapy. There was no increased sensitization to radiation in H1299 versus H1993.Conclusions
CHK1 inhibition by AZD7762 preferentially sensitizes high CHK1 expressing cells, H1299, to anti-metabolite chemotherapy as compared with low CHK1 expressing H1993 cells. Thus, CHK1 inhibitors may improve the efficacy of standard lung cancer therapies, especially for those subgroups of tumors harboring higher expression levels of CHK1 protein. 相似文献8.
Management of the lateral neck compartment in patients with sporadic medullary thyroid cancer 下载免费PDF全文
Israel Pena MD Gary L. Clayman MD Elizabeth G. Grubbs MD Jeffrey M. Bergeron BS Jr Steven G. Waguespack MD Maria E. Cabanillas MD Ramona Dadu MD Mimi I. Hu MD Bryan M. Fellman MS Yisheng Li PhD Neil D. Gross MD Stephen Y. Lai MD PhD Erich M. Sturgis MD Mark E. Zafereo MD 《Head & neck》2018,40(1):79-85
9.
Annika Treyball Audrey C. Bergeron Daniel J. Brooks Audrie L. Langlais Hina Hashmi Kenichi Nagano Deborah Barlow Ryan J. Neilson Tyler A. Roy Kathleen T. Nevola Karen L. Houseknecht Roland Baron Mary L. Bouxsein Anyonya R. Guntur Katherine J. Motyl 《Journal of bone and mineral research》2022,37(5):954-971
Although the nonselective β-blocker, propranolol, improves bone density with parathyroid hormone (PTH) treatment in mice, the mechanism of this effect is unclear. To address this, we used a combination of in vitro and in vivo approaches to address how propranolol influences bone remodeling in the context of PTH treatment. In female C57BL/6J mice, intermittent PTH and propranolol administration had complementary effects in the trabecular bone of the distal femur and fifth lumbar vertebra (L5), with combination treatment achieving microarchitectural parameters beyond that of PTH alone. Combined treatment improved the serum bone formation marker, procollagen type 1 N propeptide (P1NP), but did not impact other histomorphometric parameters relating to osteoblast function at the L5. In vitro, propranolol amplified the acute, PTH-induced, intracellular calcium signal in osteoblast-like cells. The most striking finding, however, was suppression of PTH-induced bone resorption. Despite this, PTH-induced receptor activator of nuclear factor κ-B ligand (RANKL) mRNA and protein levels were unaltered by propranolol, which led us to hypothesize that propranolol could act directly on osteoclasts. Using in situ methods, we found Adrb2 expression in osteoclasts in vivo, suggesting β-blockers may directly impact osteoclasts. Consistent with this, we found propranolol directly suppresses osteoclast differentiation in vitro. Taken together, this work suggests a strong anti-osteoclastic effect of nonselective β-blockers in vivo, indicating that combining propranolol with PTH could be beneficial to patients with extremely low bone density. © 2022 American Society for Bone and Mineral Research (ASBMR). 相似文献
10.
M G Bergeron Y Bergeron M Tardif S Marchand D Beauchamp 《Antimicrobial agents and chemotherapy》1989,33(8):1342-1345
Gentamicin is a commonly used antibiotic for the treatment of gram-negative-bacterial infections. Bacterial endotoxin is liberated during antibiotic therapy, and we have shown that endotoxemic animals accumulate more aminoglycosides in their renal parenchyma than normal animals. Vasoactive mediators, such as prostaglandins and thromboxanes, are released after endotoxin and are involved in inflammation. Indomethacin, a nonsteroidal anti-inflammatory drug known to inhibit the synthesis of these hormones, was infused intravenously as a bolus (3.0 mg/kg) or as a bolus followed by a continuous infusion (0.75 mg/kg per h) to rats given gentamicin. Levels of gentamicin in serum and kidney were increased 2 h post-antibiotic treatment in the endotoxemic animals. Renal function was not significantly disturbed. Indomethacin given as a bolus failed to correct the disturbed intrarenal pharmacokinetics of gentamicin induced by endotoxin. However, a bolus followed by continuous infusion of indomethacin resulted in low cortical and high papillary levels of antibiotic. These changes were correlated with the inhibition of prostaglandin synthesis from the kidney. These observations suggest an important role for prostaglandins in the interaction among endotoxin, aminoglycosides, and the kidney. Specific inhibitors of arachidonic acid metabolites should be investigated to further understand the mechanisms of this interaction. 相似文献