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排序方式: 共有238条查询结果,搜索用时 30 毫秒
1.
Thyroid hormones affect testicular development as well as functions like spermatogenesis and steroidogenesis, thereby influencing male fertility. Our group earlier showed that the stimulatory role of the thyroid hormone, T3, on the production of vascular endothelial growth factor (VEGF) by murine Leydig cells is mediated by steroids and hypoxia-inducible factor-1 (HIF-1α). The current study further defines the signalling pathway(s) utilised by T3 to stimulate the production of steroids, VEGF and HIF-1α in mouse Leydig tumour cell line (MLTC-1). Specific inhibitors for different signalling molecules were used to study the role of cyclic AMP (cAMP), and its downstream mediators. Expression of VEGF and HIF-1α mRNA were measured by quantitative RT-PCR; VEGF secretion by ELISA; steroid secretion by radioimmunoassay and HIF-1α protein levels by western blotting. Inhibitors of adenylate cyclase (AC), protein kinase A (PKA), sarcoma kinase (SrcK), phosphoinositide 3-kinase (PI3K) and MAP kinase kinase (MEK1/2) abolished the T3-induced increase in VEGF mRNA and protein levels. The same signalling molecules also mediated the increased production of steroids and HIF-1α protein in response to T3. Therefore, it was concluded that T3 stimulates steroid secretion and HIF-1α protein in MLTC-1 cells through the AC-cAMP-PKA-PI3K-MEK pathway, which in turn stimulate VEGF production. 相似文献
2.
Maria J. G. T. Vehreschild Surabhi Taori Simon D. Goldenberg Florian Thalhammer Emilio Bouza Joop van Oene Graham Wetherill Areti Georgopali 《European journal of clinical microbiology & infectious diseases》2018,37(11):2097-2106
Information is limited or lacking on fidaxomicin treatment of Clostridium difficile infection (CDI) in patients with inflammatory bowel disease, fulminant or life-threatening CDI, severe renal impairment, moderate-to-severe hepatic impairment and pregnancy. The ANEMONE study investigated fidaxomicin use in a routine clinical setting, focusing on these medical conditions of specific interest (MCSIs). This retrospective, post-authorisation study reviewed hospital records from Austria, Germany, Spain and the UK (June 2012–June 2015), collecting data from hospital admission to 30 days after last fidaxomicin dose. The primary objective was to identify the proportion of fidaxomicin-treated patients with MCSIs. Secondary objectives were to describe 30-day mortality, changes in ECG and laboratory parameters, fidaxomicin exposure and CDI response (resolution of diarrhoea; 30-day recurrence). 45.3% (261/576) of patients had ≥?1 MCSI. Thirty-day mortality (post-first dose) was 17.0% (98/576) in the total population and slightly higher (24.6–27.6%) in patients with fulminant CDI or severe renal impairment. 29.6% (24/81) deaths of known cause were attributable to CDI. Of changes in laboratory parameters or ECG findings, only a decrease in leucocyte counts appeared associated with fidaxomicin, consistent with a positive treatment response. Diarrhoea resolved in 78.0% (404/518) of treatment episodes; diarrhoea resolution was lowest in patients with fulminant CDI (investigator-defined, 67.5%, 56/88) and severe renal impairment (68.0%, 68/100). Thirty-day recurrence (18.8%, 79/420) was similar across MCSI subgroups. Although almost half of fidaxomicin-treated patients had ≥?1 MCSI, the majority of patients in all subgroups had positive responses to treatment, and no particular safety concerns were identified. 相似文献
3.
Brett D. Rosenthal Tyler J. Jenkins Arjun Ranade Surabhi Bhatt Wellington K. Hsu Alpesh A. Patel 《The spine journal》2019,19(1):157-162
Background Context
Despite the prevalence and importance of myelopathy, there is a paucity of objective and quantitative clinical measures. The most commonly used diagnostic tools available are nonquantitative physical exam findings (eg, pathologic reflexes, and gait disturbance) and subjective scoring systems (eg, modified Japanese Orthopaedic Association [mJOA]). A decline in fine motor coordination is a hallmark of early myelopathy, which may be useful for quantitative testing.Purpose
To identify if a novel tablet application could provide a quantitative measure of upper extremity dysfunction in cervical spondylotic myelopathy.Study Design/Setting
Prospective cohort study Patient Sample: Adult patients with a diagnosis of cervical spondylotic myelopathy from a board-certified, spine surgeon were compared with age-matched, healthy, and adult control patients. Outcome Measures: Self-reported function was assessed via the mJOA. Upper extremity function was measured via the fine motor skills (FiMS) tablet test.Methods
Subjects and controls prospectively completed the mJOA paper survey and the FiMS tablet testing, which consisted of four challenges.Results
After age-matching, 65 controls and 28 myelopathic patients were available for comparison. The mean mJOA was 13.5 ± 2.9 in the myelopathic cohort and 17.3 ± 1.1 in the control cohort (p < .0001). The average scores for challenges 1–4 in control patients were 24.4, 16.3, 3.2, and 6.6, respectively, whereas the average scores for the myelopathic patients were 16.6, 10.5, 1.4, and 1.8, respectively (p values for all four challenges <.001). Based upon the 15 control subjects who repeated FiMS testing four sequential times, intrarater reliability was excellent, yielding an interclass correlation coefficient of 0.88Conclusions
The FiMS tablet application produced significantly lower scores in a myelopathic cohort when compared with an age-matched control cohort. This is true for all four challenges in the FiMS tablet application. The test can be completed in 1.5 minutes, producing a reliable, quantitative measure of cervical myelopathy upper extremity function. In summary, the FiMS tablet application is a novel, easily administered, objectively quantifiable test for analyzing cervical spondylotic myelopathy. 相似文献4.
Recalcitrant hypertrophic herpes genitalis in HIV‐infected patient successfully treated with topical imiquimod 下载免费PDF全文
Herpes Simplex Virus type 2 is the primary cause of genital ulceration worldwide. The presence of atypical features like deep ulcerations, hypertrophic, or pseudotumoural lesions or unusual location can be a marker for co‐infection with HIV. These immunocompromised patients are usually resistant to the conventional antiviral treatment. We present a case of an HIV‐infected patient with hypertrophic herpes genitalis, refractory to conventional oral antiviral therapy, who was successfully treated with a combination of oral valcyclovir and topical application of 5% imiquimod. 相似文献
5.
Srivastava Sharad Tiwari Surabhi Srivastava Nishi Verma Shikhar Rawat Ajay Kumar Singh 《Proceedings of the National Academy of Sciences, India. Section B.》2019,89(3):1057-1066
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - Centella asiatica (L.) Urb. (Apiaceae) with high commercial value is an important herb in traditional Indian... 相似文献
6.
Madhwal S Goldberg J Barcena J Guha A Gogate P Cmolik B Elgudin Y 《The Annals of thoracic surgery》2012,93(3):974-977
Idiopathic hypereosinophilic syndrome (HES) is a rare multisystem condition characterized by dysregulated overproduction of eosinophils. Cardiac involvement in HES is characterized by necrosis from infiltration of eosinophils and thrombus formation and, in the late stage, by fibrosis and chronic valvular regurgitation. We report a very unusual presentation of idiopathic HES with acute mitral regurgitation due to papillary muscle rupture. The transesophageal echocardiogram was suggestive of a flail posterior leaflet and suspicious for endocarditis. Intraoperatively, papillary muscle rupture was seen and the patient underwent mitral valve replacement. The pathologic examination of the valve revealed eosinophilic infiltration of the papillary muscle. The patient was treated with steroids and responded well clinically. 相似文献
7.
Draft genome of the red harvester ant Pogonomyrmex barbatus 总被引:3,自引:0,他引:3
Smith CR Smith CD Robertson HM Helmkampf M Zimin A Yandell M Holt C Hu H Abouheif E Benton R Cash E Croset V Currie CR Elhaik E Elsik CG Favé MJ Fernandes V Gibson JD Graur D Gronenberg W Grubbs KJ Hagen DE Viniegra AS Johnson BR Johnson RM Khila A Kim JW Mathis KA Munoz-Torres MC Murphy MC Mustard JA Nakamura R Niehuis O Nigam S Overson RP Placek JE Rajakumar R Reese JT Suen G Tao S Torres CW Tsutsui ND Viljakainen L Wolschin F Gadau J 《Proceedings of the National Academy of Sciences of the United States of America》2011,108(14):5667-5672
We report the draft genome sequence of the red harvester ant, Pogonomyrmex barbatus. The genome was sequenced using 454 pyrosequencing, and the current assembly and annotation were completed in less than 1 y. Analyses of conserved gene groups (more than 1,200 manually annotated genes to date) suggest a high-quality assembly and annotation comparable to recently sequenced insect genomes using Sanger sequencing. The red harvester ant is a model for studying reproductive division of labor, phenotypic plasticity, and sociogenomics. Although the genome of P. barbatus is similar to other sequenced hymenopterans (Apis mellifera and Nasonia vitripennis) in GC content and compositional organization, and possesses a complete CpG methylation toolkit, its predicted genomic CpG content differs markedly from the other hymenopterans. Gene networks involved in generating key differences between the queen and worker castes (e.g., wings and ovaries) show signatures of increased methylation and suggest that ants and bees may have independently co-opted the same gene regulatory mechanisms for reproductive division of labor. Gene family expansions (e.g., 344 functional odorant receptors) and pseudogene accumulation in chemoreception and P450 genes compared with A. mellifera and N. vitripennis are consistent with major life-history changes during the adaptive radiation of Pogonomyrmex spp., perhaps in parallel with the development of the North American deserts. 相似文献
8.
Meenakshi Lallar Haq ul Anam Rajesh Nandal Sunder Pal Singh Surabhi Katyal 《Journal of obstetrics and gynaecology of India》2015,65(1):17-22
Objectives
To compare intravenous paracetamol and intramuscular tramadol as labor analgesics.Methods
This prospective-randomized study conducted in 200 primigravidae in active labor, distributed into two groups of 100 women each with one receiving intravenous 1,000 mg Paracetamol and other 100 mg intramuscular tramadol. Pain intensity is recorded by McGills scale before, one and 3 h after drug administration. Perinatal outcome is recorded.Results
No difference in pain intensity is seen before drug administration. After 1 h of drug administration, in paracetamol group, 4 % women had horrible pain, and 29 % had distressing pain, while in tramadol group, 30 % women had horrible pain, and 60 % had distressing pain. After 3 h of drug administration, in paracetamol group, 26 % had distressing pain, while in tramadol group, 51 % women had horrible pain, and 35 % had distressing pain. Labor duration in paracetamol and tramadol group was 4.3 and 5.9 h, respectively. In paracetamol group, nausea is seen in 2.2 % and vomiting in 1.1 %, while in tramadol group, nausea is seen in 6.4 % and vomiting in 4.3 %.Conclusions
Intravenous paracetamol is more effective labor analgesic with fewer maternal adverse effects and shortens labor as compared to intramuscular tramadol.9.
Objective
The aim of this study was to find lipid profile and hormone levels in patients with severe acne vulgaris and to correlate lipid profile with hormone levels.Design and methods
Prospective study was performed in the 60 patients of severe acne vulgaris. Lipid profile and serum testosterone, estrogen, and progesterone levels were measured in three consecutive menstrual cycles during luteal phase (19th to 21st day). Their results were compared with a group of 60 age-matched healthy controls.Results
Total cholesterol (TC), LDL-C, testosterone, and progesterone levels were higher in patients. Estrogen and HDL-C levels were decreased in patients. Positive correlation was found between testosterone and TC and LDL-C. Estrogen levels were positively correlated with VLDL-C, triglyceride levels, and HDL-C and negatively correlated with TC and LDL-C.Conclusions
These abnormalities must be considered in the pathogenesis of the disease and must be taken into account for the treatment of patients of acne. 相似文献10.
Satyaswarup Tripathy Arshad H. Khan Surabhi Sharma 《European journal of plastic surgery》2010,33(1):23-28
Defects around the elbow region are often irregular with exposed nerves, tendons, bones/joint, and implants. Skin grafts,
though possible in some cases, must be avoided, as it requires prolonged splintage and physiotherapy to prevent recurrence
and elbow stiffness. Distant flaps require multiple stages, and free flaps are rarely indicated for elbow defects. Muscle
flaps are bulky. To overcome these problems, distally based fasciocutaneous flaps from medial and lateral sides of the arm
based on posterior ulnar recurrent artery and radial recurrent artery, respectively, were used. Ten patients were studied.
Age of the patients ranged from 10 to 40 years. Five were male, and five were female. Radial and ulnar recurrent flaps were
used in five patients each. The largest flap size was 18 × 8 cm, and the smallest was 10 × 6 cm. The flaps were islanded in
four cases, and in six cases, they were used as pedicled flaps. Skin grafts were used in 90% of the patients because large
flaps were required for resurfacing; also, our patients had thinner arms with less girth. All flaps survived well. One island
flap had venous congestion and marginal necrosis, which eventually healed. A significant improvement of mobility and elbow
movement was noted in all patients. These flaps can be raised even from the previously burnt skin, as the pedicle is located
deep. These flaps have the following advantages: The flap is reliable (deeply located consistent vascular pedicle), can cover
both the flexor and extensor surface of the elbow joint, avoids prolonged splintage, elbow stiffness, and recurrence, good
aesthetic, and has functional results. 相似文献