全文获取类型
收费全文 | 15379篇 |
免费 | 613篇 |
国内免费 | 88篇 |
专业分类
耳鼻咽喉 | 169篇 |
儿科学 | 293篇 |
妇产科学 | 166篇 |
基础医学 | 1763篇 |
口腔科学 | 254篇 |
临床医学 | 1109篇 |
内科学 | 3914篇 |
皮肤病学 | 145篇 |
神经病学 | 1136篇 |
特种医学 | 720篇 |
外科学 | 2720篇 |
综合类 | 59篇 |
预防医学 | 367篇 |
眼科学 | 537篇 |
药学 | 989篇 |
中国医学 | 39篇 |
肿瘤学 | 1700篇 |
出版年
2021年 | 196篇 |
2020年 | 99篇 |
2019年 | 167篇 |
2018年 | 203篇 |
2017年 | 189篇 |
2016年 | 264篇 |
2015年 | 248篇 |
2014年 | 359篇 |
2013年 | 466篇 |
2012年 | 755篇 |
2011年 | 838篇 |
2010年 | 505篇 |
2009年 | 468篇 |
2008年 | 807篇 |
2007年 | 880篇 |
2006年 | 877篇 |
2005年 | 906篇 |
2004年 | 957篇 |
2003年 | 941篇 |
2002年 | 968篇 |
2001年 | 390篇 |
2000年 | 412篇 |
1999年 | 360篇 |
1998年 | 221篇 |
1997年 | 188篇 |
1996年 | 166篇 |
1995年 | 167篇 |
1994年 | 155篇 |
1993年 | 154篇 |
1992年 | 263篇 |
1991年 | 231篇 |
1990年 | 212篇 |
1989年 | 218篇 |
1988年 | 211篇 |
1987年 | 138篇 |
1986年 | 160篇 |
1985年 | 155篇 |
1984年 | 107篇 |
1983年 | 91篇 |
1982年 | 47篇 |
1981年 | 47篇 |
1980年 | 50篇 |
1979年 | 99篇 |
1978年 | 66篇 |
1977年 | 61篇 |
1974年 | 54篇 |
1973年 | 55篇 |
1972年 | 49篇 |
1971年 | 67篇 |
1969年 | 47篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
1.
2.
3.
Comprehensive analysis of gene expression of isolated pancreatic islets during pretransplant culture
Junichiro Haga Naoya Sato Takayuki Anazawa Takashi Kimura Akira Kenjo Mitsukazu Gotoh Shigeru Marubashi 《Fukushima journal of medical science》2021,67(1):17
Background: The aim of this study was to investigate the effect of pretransplant culture on the survival of pancreatic islet grafts, and to determine the biological characteristics of isolated islets during pretransplant culture. Methods: The survival of islets from Wistar rats, transplanted to diabetic C57BL/B6 mice, was compared between fresh islets and cultured islets. A comprehensive gene expression analysis was employed to investigate biological processes during pretransplant culture, and in vitro validation studies were performed. Results: Survival of cultured xenografts was significantly prolonged as compared to that of fresh islets (fresh: 12.5 ± 1.9 days, 1-day cultured: 16.0 ± 1.3 days (p= 0.017), 3-day cultured: 17.0 ± 2.6 days (p= 0.014)). Comprehensive gene expression analysis identified significant upregulation of annotated functions associated with inflammation in cultured groups. Six proinflammatory genes, including heme oxygenase 1 (HO-1) and IL-6, were significantly upregulated during culture. Validation studies revealed significantly higher levels of IL-6 in the supernatant of cultured islets and HO-1 in the cultured islets when compared with fresh islets. Conclusion: Transplantation of cultured islets induced significant but minimal prolongation of graft survival in xenogeneic combinations. Comprehensive analysis of gene expression in cultured islets showed biological processes associated with proinflammation during culture. 相似文献
4.
5.
6.
7.
8.
Yukitomo Ishi Shigeru Yamaguchi Michiharu Yoshida Hiroaki Motegi Hiroyuki Kobayashi Shunsuke Terasaka Kiyohiro Houkin 《Journal of neuroradiology. Journal de neuroradiologie》2021,48(4):266-270
Background and purposeMost individuals with optic pathway/hypothalamic pilocytic astrocytoma (OPHPA) harbor either the BRAF V600E mutation or KIAA1549-BRAF fusion (K-B). This study aimed to investigate the imaging characteristics of OPHPA in relation to BRAF alteration status.Materials and methodsSeven cases of OPHPA harboring either the BRAF V600E mutation or K-B fusion were included in the study. Preoperative magnetic resonance imaging (MRI) was assessed for degree of T2 hyperintensity on T2-weighted images (T2WI) and the ratio of nonenhancing T2 or fluid-attenuated inversion recovery (FLAIR) hyperintense area to the contrast enhanced area (CE) on gadolinium-enhanced-T1 weighted images (T2/FLAIR-CE mismatch). The T2 signal intensity was normalized to cerebrospinal fluid (T2/CSF) for both the V600E and K-B group and compared. T2/FLAIR-CE mismatch was assessed by calculating the proportion of the tumor volume of nonenhancing high T2 signal intensity to the whole lesion (nonenhancing and enhancing components).ResultsFour and three cases of OPHPA harboring the BRAF V600E mutation and K-B, respectively, were analyzed. The T2/CSF value was higher in the K-B group than in the V600E group. Moreover, the V600E group had a larger T2/FLAIR-CE mismatch than the K-B group.ConclusionsThe BRAF alteration status in individuals with OPHPA was associated with preoperative MRI by focusing on T2 signal intensity and T2/FLAIR-CE mismatch. The BRAF V600E mutation was associated with a lower T2/CSF value and larger T2/FLAIR-CE mismatch, whereas K-B fusion was associated with a higher T2/CSF value and smaller T2/FLAIR-CE mismatch. 相似文献
9.
10.