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1.
CHROMOSOME CHANGES IN PATIENTS WITH CHRONIC BENZENE POISONING   总被引:3,自引:0,他引:3  
Chromosome changes are studied in 21 cases of chronic benzene poisoning, both hypodiploid and hyperdiploid celLs are significantly increased. Among the hypodiploid cells, deletion of Group C,E and G chromosomes is observed. Analysis of hyperdiploid cells reveals extra Group C' and E chromosomes. The chromatid gaps and breaks are mostly found in poisoned cases, but frag- ments and minutes are less common. The chromosome abnormalities induced by chronic benzene intoxication may be considered further proof of benzene metabolite acting chiefly through changes in the nucleic acid metabolism.  相似文献
2.
一阶导数分光光度法测定氯地滴耳液中氯霉素的含量   总被引:2,自引:0,他引:2  
Determination of chloramphenicol in the ear drops by first derivative spectrometry was studied. The detecting wavelength was 303.0 nm. The linear range was 0-65.2 micrograms.ml-1 (r = 0.9999). The average recovery was 99.84%, RSD = 0.37% (n = 8). The method is simple and accurate.  相似文献
3.
In order to study effects of cigarette smoking and smoking cessation on plasma constituents and enzyme activities related to oxidative stress, 1255 smokers and 524 healthy non-smokers were investigated in terms of plasma levels of lipoperoxides (LPO), nitric oxide (NO), vitamin C (VC), vitamin E (VE) and beta-carotene (beta-CAR). Additionally, erythrocytes were examined to determine the level of LPO, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). The results showed that, when compared with the average values of the non-smoker group, the average plasma values of LPO, NO and the average erythrocyte value of LPO in the smoker group were significantly increased (P < 0. 001), while the average plasma values of VC, VE, beta-CAR, and the average erythrocyte activities of SOD, CAT, GSH-Px were significantly decreased (P < 0.001). A linear regression and correlation analysis for 65 male smokers who were all 40 years old showed that with longer smoking duration and greater daily smoking quantity, the plasma values of LPO, NO and the erythrocyte value of LPO were elevated, while the plasma values of VC, VE, beta-CAR and erythrocyte values of SOD, CAT, GSH-Px were decreased. In a group of 73 smokers who stopped smoking completely for six months, the average plasma values of LPO, NO and the average erythrocyte value of LPO decreased, although they were still significantly higher than those in the matched non-smoker group (P < 0.05). Additionally, the average plasma values of VC, VE, beta-CAR and the average erythrocyte values of SOD, CAT, GSH-Px increased, although they were still significantly lower than those in the matched non-smoker group (P < 0.05). However, after smoking cessation for one year the above average values were not significantly different from those in the matched non-smoker group (P > 0.05). This finding indicates that the markedly increased oxidative stress in smokers might gradually return to normal but only after a long period of smoking cessation. In conclusion, in the bodies of smokers a series of free radical chain reactions were gravely aggravated, the dynamic balance between oxidation and antioxidation was seriously disrupted, and oxidative stress was clearly exacerbated, which is closely related to many disorders or diseases in smokers. The present study underscored the need, urgency and importance of complete smoking cessation.  相似文献
4.
输卵管结扎术后综合征的病因及防治   总被引:1,自引:0,他引:1  
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bcl-2基因转染小鼠造血干细胞重建造血的生物学特性   总被引:1,自引:0,他引:1  
目的 观察bcl-2基因转染小鼠造血干细胞重建造血的小鼠对放化疗的抵抗作用。方法 (1)含bcl-2cDNA的逆转录病毒载体质粒,经脂质体介导转染PA317包装细胞,通过G418筛选和克隆,获得高病毒滴度的细胞株(D3);(2)用D3细胞株的病毒上清转染小鼠胚胎肝造血干细胞;(3)经致死剂量照射小全注bcl-2转 造血干细胞重建造血;(5)采用脾集落形成和再增殖能力观察bcl-2转因干细胞重建造血  相似文献
7.
We obtained highly repeated DNA fragments from a rabbit by using restriction endonuclease HindIII digestion of chromosomal DNA; the smallest highly repeated DNA fragment was cloned into vector plasmid pUC12. We used the recombinant plasmid to transform E. coli JM101, selected and identified the positive clone by DNA probe in situ hybridization as well as Southern blotting. We obtained a recombinant clone [termed pRAb (Hind III)-1] which contains the highly repeated DNA fragment [termed RAb (Hind III)-1]. The complete nucleotide sequence of the 345bp RAb (Hind III)-1 fragment was determined by the dideoxynucleotide termination sequencing method. We analyzed the characteristics of the structure of the sequence with a microcomputer, and the results suggested that the RAb (Hind III)-1 sequence is quite different from alphoid DNA of primates.  相似文献
8.
This study was designed to determine whether or not atrial natriuretic factor (ANF) is present in the vascular walls and to observe the differences in ANF between control (WKY) and stroke-prone spontaneously hypertensive rats (SHRsp). It was found that ANF is indeed present in the vascular wall of the distal aorta. HPLC analysis of the extracts from cultured aortic smooth muscle cells (ASMC) and medium revealed that intracellular ANF was mainly in the form of ANF(1-126), at levels of 0.82 +/- 0.03 (SHRsp) and 1.04 +/- 0.10 ng/10(6) cells (WKY), while the major form in the medium was ANF(99-126), at levels of 0.40 +/- 0.06 and 0.60 +/- 0.06 ng/10(6) cells, respectively. Both forms were present in smaller amounts in SHRsp than in WKY rats. On the contrary, both renin activity and angiotensin I concentrations in SHRsp cells were significantly higher than those in the WKY controls. In addition, immunocytochemistry showed positive ANF staining in cultured ASMC of both strains. The results suggest that ANF can be synthesized and secreted by cultured ASMC from rats.  相似文献
9.
目的:建立HPLC测定家犬血浆中萘哌地尔浓度的方法,研究大剂量萘哌地尔胶囊在家犬体内的药物动力学,方法:健康家犬5只,单剂量给予萘哌地尔胶囊200mg后,在不同时间点从后肢静脉取血,血浆样品碱化后经乙醚提取,以乙腈:磷酸盐缓冲液(pH6.5)(60:40)为流动相,由ODSC18分析柱分离测定,紫外230nm为检测波长,维拉帕米为内标,血药浓度数据用3p97药物动力学程度处理,结果:线性范围为10-1200ng/ml^-1;方法回收率为98.83%-101。50%,日间RSD≤5.56%,日内RSD≤3.30%,单剂量给予家犬萘哌地尔胶囊200mg后,血药浓度随时间变化规律符合一室开放模型,T1/2Ke为1.91-4.99h,Tmax为1.87-3.21h,Cmax为338.79-414.04ng.ml^-1。结论:本方法简便,回收率高,重现性好,用于大剂量萘哌地尔在动物体内的药物动力学研究,切实可行。  相似文献
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