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1.
Zhang WG  Lü DC  Fu CY  Qü W 《Zhonghua yi xue za zhi》2006,86(15):1065-1068
目的观察壳聚糖复合他克莫司(FK506)缓释鞘管结合3mm小间隙桥接神经对神经再生的影响。方法将45只雄性SD大鼠,随机分成3组,切断双侧坐骨神经制成坐骨神经再生室模型,并保留断端间隙为3mm。实验所用得桥接材料分别是:硅胶管、壳聚糖鞘管、壳聚糖复合FK506缓释鞘管。分别于术后6、8、12周观察桥接材料周围的瘢痕形成及吸收情况,并行神经电生理、组织学观察、图象分析、腓肠肌湿重检测比较各组大鼠坐骨神经的再生与功能恢复情况。结果术后大体标本观察显示:对照组再生室与周围组织粘连较重;壳聚糖鞘管组、壳聚糖复合FK506鞘管组神经桥接处较易与周围组织剥离,粘连轻微。6周时各组再生室均尚存;8周时壳聚糖鞘管组、壳聚糖复合FK506鞘管组桥接材料开始吸收;12周时壳聚糖鞘管组、壳聚糖复合FK506鞘管组桥接材料不完整或仅有碎片残留,神经连续性建立,且与周围组织无明显粘连。评估神经再生的运动神经传导速度、复合肌肉动作电位、潜伏期检测指标,壳聚糖复合FK506鞘管组(10.2m/s±0.8m/s、4.3mV±0.3mV、1.9ms±0.4ms)明显优于对照组(4.2ms±0.5ms、1.2mV±0.3mV、7.5ms±0.4mV)、壳聚糖鞘管组(9.5ms±0.3ms、2.7mV±0.3mV、3.1ms±0.4ms),P<0.05。壳聚糖鞘管组虽然各指标均数优于对照组,但之间差异无统计学意义。结论应用壳聚糖复合FK506缓释鞘管桥接大鼠坐骨神经,在体内稳定2个月以上开始降解,能够明显促进神经再生与功能恢复,且降解产物为多糖类,不影响神经再生微环境。  相似文献
2.
老年男性人群前列腺症状调查   总被引:12,自引:0,他引:12  
为了解老年男性的前列腺症状情况,用国际前列腺症状评分对成都地区412名60岁以上老年男性的前列腺症状进行了调查,其中城市116名,乡村296名,结果显示城乡之间老年男性的前列腺症状评分和生活质量评分均有显著性差异;症状评分在不同年龄组间无统计学差异,但高龄组平均分和高分段所上百分比均高于低于龄组。  相似文献
3.
OBJECTIVE: To investigate the growth behavior and metastatic pattern of murine cervix cancer U14 transfected with human papillomavirus(HPV) in inbred 615-strain mouse. METHODS: We transfected HPV 16 E6 and E7 genes into mouse cervix carcinoma cell strain NO. 14(U14) by electroporation and liposome, respectively. The transfectants were selected by G418, and several high-expressed HPV16 E6 and E7 clonal cell lines (E6+ U14, E7+ U14) were detected by PCR and by immunohistochemistry. We transplanted those cells into inbred 615-strain mice both subcutaneously and intraperitoneally to observe the growth behavior and metastasis of them. RESULTS: The durations of tumor appearance were 5-7 d, 11-14 d, and 8-10 d after having been transplanted subcutaneously with wild type U14, E6+ U14, and E7+ U14, respectively(P < 0.05). The mean survival times of mice were 29 d, 43 d, and 35 d, respectively. Metastasis could be found both in lymph nodes (90%, 30%, and 40%, respectively) and lungs (60%, 10%, and 20%, respectively). After intraperitoneal inoculation, the mean survival durations of mice were 14.2 d, 20.6 d and 18.3 d. We could not find metastasis both in lymph nodes and lungs. CONCLUSION: Murine cervix cancer U14 cells transfected with HPV16 E6 and E7 have different growth behavior and metastatic patterns after transplanted in inbred mice, which provide useful models for studying their immunotherapy or other strategies for cervical cancer with E6 and E7 as a target.  相似文献
4.
目的 研究体内直接注身携带白细胞介素12(mIL-12)逆转录病毒载体包装细胞株对体内肝癌细胞生长抑制作用,探索基因治疗新途径。方法 构建携带mIL-12的逆转录病毒载体,将载体转染包装细胞株,用该细胞株分别在大鼠腹腔内与肝癌细胞株混合接种,在肝癌局部及脾内直接注射,观察对肝癌生长抑制作用及其对大鼠毒性反应、免设功能变化。结果 应用该细胞株与肝癌细胞株在腹腔进行混合接种,1:10以下治疗组可使大鼠  相似文献
5.
TGFβ1对人血管内皮细胞整合素β3及粘着斑激酶表达的影响   总被引:3,自引:0,他引:3  
OBJECTIVE: To observe the effects of transforming growth factors beta 1 (TGF beta 1) on the expression of integrin beta 3 and the activity of focal adhesion kinase (FAK). METHODS: This study was performed on cultured human endothelial cells (EC) by using cell-ELISA and immunoprecipitation-tyrosine kinase assay respectively. RESULTS: Under the stimulus of TGF beta 1, there a dose-dependent increase in the expression of integrin beta 3 chain in the surface of EC. And after the cultured EC were treated with 5 ng/ml or 10 ng/ml TGF beta 1 for 6 or 24 hours, the FAK activity in EC increased significantly as compared with the control group (P < 0.05). CONCLUSION: The expression of integrin beta 3 and the activity of FAK on EC were regulated by TGF beta 1, and this regulation may be important in cell adherence, angiogenesis, and in the pathophysiology of atherosclerosis.  相似文献
6.
三黄片中有效成分的薄层色谱测定   总被引:2,自引:0,他引:2  
本文采用TLCS法测定了三黄片中大黄素、小檗碱和黄苓甙等有效成分的含量。以硅胶G为吸附剂,先用苯-乙酸乙酯-乙酸(75∶25∶1.5)展开分高大黄酸和大黄素,再用氯仿-乙酸乙酯-甲醇-甲酸(7∶3∶1∶1)分离小檗碱和黄苓甙,于CS-910薄层扫描仪上,在选定的波长处测定各组分的含量。本法操作简便,回收率在94%以上,变异系数小于4%。  相似文献
7.
目的 对能与人血管内皮生长因子(VEGF)特异结合,并对体内有抑瘤活性的单克隆抗体(E11)进行基因工程改造,构建小分子的单链抗体(ScFv),为临床应用奠定基础。方法 通过逆转录及多聚酶链式反应,扩增并克隆E11的可变区基因片段。用一编码亲水肽接头的DNA片段将E11轻、重链可变区基因连接,克建表达载体PET-15YV,并导入大肠杆菌BL21(DE3)中进行表达。表达产物经变性及复性后,用免疫组  相似文献
8.
This paper reports the successful experiences of in vitro and in vivo studies on endoscopic fragmentation of gastric bezoars using a laser-ignited mini-explosive device. This new technique has been used clinically in 31 patients with gastric bezoars since January 1988. All patients were completely cured without any complication. The relationship between gastric bezoars and ulcers, and the technique of treatment were discussed.
  相似文献
9.
10.
This article reports the therapeutic effectsof point injection with strychnine and vitaminB_(12) combined with electro-acupuncture inmanagement of 300 cases of facial paralysisperipherica.Our results show a total recoveryrate of 52.7 percent,with the greatest efficacyseen in mild cases starting treatment within onemonth after onset of the disease.Eighty-threepercent of the patients recovered or basicallyrecovered within three courses of treatment(30sessions),and the recoveries account for 90.5percent of the total recoveries.  相似文献
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