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1.
褪黑激素对小鼠吗啡戒断反应及血清单胺类递质的抑制作用   总被引:19,自引:0,他引:19  
目的观察褪黑激素对小鼠吗啡戒断反应及血清单胺类递质的影响。方法皮下注射定量吗啡,建立小鼠吗啡依赖模型;腹腔注射纳络酮催瘾。根据小鼠戒断反应中出现跳跃反应的潜伏期和跳跃次数评定戒断反应的强度。用高效液相色谱电化学检测法(HPLCECD)检测血中去甲肾上腺素和多巴胺含量。结果4种不同剂量(25、50、100、200mg/kg)的褪黑激素对小鼠吗啡戒断反应有明显抑制作用,并呈很好的量效关系。100mg/kg的褪黑激素可抑制吗啡戒断反应引起的血清中去甲肾上腺素及多巴胺浓度的升高。结论褪黑激素可抑制小鼠吗啡戒断反应,并抑制戒断反应引起血清单胺类递质的升高。  相似文献
2.
Bao Y  Lu D  Xu H  Shi Q  Qiu X  Xue J 《Chinese medical journal》1998,111(6):527-530
OBJECTIVE: To establish the polymorphism of DXS102 locus from Xq26.3-27.1 in Chinese population for the gene diagnosis in Hemophilia B family. METHODS: DNA was extracted from blood samples obtained from Shanghai unrelated volunteer donors with phenol-chloroform method. A total of 23x chromosomes (154 from females, 80 from males) were studied. A hemophilia B family in which a hemophilia B patient has received gene therapy was analyzed. The polymorphism of DXS102 locus in Chinese population was determined with amplified fragment length polymorphisms assay (Amp-FLP), denaturing polyacrylamide gel electrophoresis, silver stain detection. Short tandem repeats (STRs) linkage analysis was used to conduct gene diagnosis in hemophilia B family. RESULTS: Eight alleles were found at DXS102 locus, of which two alleles were first reported. The repeated number of AC dinucleotide ranges from 13 to 21. And the values of the observed heterozygosity, calculated heterozygosity and polymorphism information content(PIC) were 0.87, 0.80, 0.80 respectively. It was also found that the difference of the allele frequencies of DXS102 in Chinese and European populations was significant. By using the linkage analysis of the DXS102 locus, a family with a hemophilia B patient receiving gene therapy in 1994 was analyzed and meanwhile a carrier in that family was then detected. CONCLUSIONS: The polymorphism of DXS102 locus reveals significant difference between Chinese and European populations. DXS102 locus can be used as a promising marker for gene diagnosis in hemophilia B family.  相似文献
3.
4.
D Ouyang  H Qiu  X Lu  M Long 《湖南医科大学学报》1999,24(2):181-2, 210
The reasons of complications and deaths in 98 aged patients with obstructive jaundice were analysed in the paper. Among them, 52 cases were diagnosed as benign obstruction, 46 cases as malignant obstruction; 92 patients were treated by surgical management(15 died), 6 patients were treated without operation(4 died). The results showed that effective management on time and intensive perioperative care are important to minimize the mortality rate. Malignant obstructive jaundice was more harmful to the patients' renal function no the mortality rate would be increased. The operations, internal drainage and pressure reduction of biliary duct, were performed on time, thus the survival rate was improve.  相似文献
5.
Craniocerical junction of 42 patients with Arnold-Chiari I malformation (ACM-1) and 41 normal controls were studied. In addition to the decendent tonsil, all the components in the posterior cranial fossa including the 4th ventricle, oblongata medulla and the sinus confluence of patients with ACM- 1 were decended. Although the spinal foramen magnum anterior -posterior diameter was smaller but not statistically different from the control. The results suggest that the main pathology of ACM- 1 is the shallow and small posterior cranial fossa and the resulted organ decendent as a whole.  相似文献
6.
T Lin  K Li  C Li  C Qiu  X Zhan 《华西医科大学学报》2001,32(2):315-6, 324
OBJECTIVE: This experiment was designed to make known wether the photostability of drugs in daylight can be obtained from lamplight exposure. METHODS: A self made cumulative illuminometer via pulse counting method was used for measuring cumulative illuminance of daylight to investige the photodegradation of Hydrocortisone injection in various lamp light and daylight; the equivalent influences of different light sources on the photostability of the injection were obtained, and the shelf-life in indoor daylight of the drug was predicted. RESULTS: The photodegradation of Hydrocortisone injection obeys zero order kinetics: C = C0-kEt; the shelf-life in indoor daylight of the injection in nude ampoules was predicted as 36 days and is comparable to 35 days in a long-term storage test. CONCLUSION: The photostability of drugs in daylight can be obtained from lamplight exposure experiment.  相似文献
7.
L Qiu  X Qin  Y Fu  Y Zheng 《华西医科大学学报》1999,30(3):310-1, 353
This experiment was done with a view to exploring the etiologic mechanism and new therapy of otitis media effusion (OME). The amount of lecithin, which is representative of the surfactants in the nasopharynx irrigation wash liquid, was determined by photometry in 40 cases OME and 40 normal controls. The results showed the surfactant of otitis media effusion (8.637 +/- 2.730 mg/L) was significantly lower than that of normal (39.212 +/- 2.437 mg/L) in the nasopharynx irrigation wash liquid (P < 0.01). The surfactant of child group of OME (8.062 +/- 2.925 mg/L) significantly lower than that of normal control(39.787 +/- 2.557 mg/L, P < 0.01). The surfactant of otitis media effusion in the nasopharynx irrigation wash liquid was not associated with the course of OME. These data suggest that the surfactant reduction in the nasopharynx could indirectly reflect the level of the surfactant in the eustachian tube and the middle ear and influence the eustachian tube function. Surfactant reduction may be one of the etiologic mechanisms of OME. Increasing the surfactant in the nasopharynx and eustachian tube could be beneficial to reducing the persistent otitis media effusion.  相似文献
8.
OBJECTIVE: To establish the replicated-deficient recombinant adenovirus-mediated thymidine kinase/acyclovir (Adtk/ACV) system and to evaluate its suicide effect on rat C6 brain gliomas in vitro and in vivo. METHODS: The plasmid pAdtk and pJM17 were co-infected into 293 cells (adenovector packaging cells) and the results were identified by polymerase chain reaction (PCR) assay. After the glioma C6 cells were transduced by Adtk at different multiplicity of infection (MOI) and exposed to different concentrations of ACV or gancyclovir (GCV), the cell survival curves were studied, and the cell surface was observed with scanning electronic microscopy (SEM). C6 gliomas in vivo at different inoculation days were injected with Adtk intratumorally and ACV intraperitoneally daily, and the survival duration and histologic changes of the rats were observed. RESULTS: The infectious Adtk virions had a suicide effect which was enhanced with the increase in MOIs of Adtk and ACV doses along with bystander effect. Under scanning electronic microscope, special pathologic changes were observed. ACV had a similar effect as GCV but a higher dose was used. The survival duration in day 3, day 6 and day 8 groups exceeded 90 days, and the rats in day 10 group survived 28.5 +/- 4.6 days, but the survival duration in untreated C6 group and AdLacZ/ACV (adenovirus-mediated LacZ/ACV) treated group were 16.8 +/- 3.1 and 14.0 +/- 2.2 days respectively. CONCLUSION: Adtk/ACV system can effectively kill the rat brain gliomas in vitro and in vivo.  相似文献
9.
The effect of Kupffer cell blockade on hepatic function during sepsis was evaluated in this study. Methyl palmitate suspension 100 mg/100 g administered intravenously suppressed the phagocytic activity as the phagocytic index K decreased from 0.0493 +/- 0.0089 to 0.0150 +/- 0.0035 in rats. Sepsis was produced by the method of cecal ligation and needling perforation (CLP). At the end of 15 hours after CLP the hepatic adenosine triphosphate (ATP) level and ketone body ratio decreased significantly. But in rats pretreated with methyl palmitate 24 hours prior to CLP, the ATP level returned to the normal control level (1.6906 +/- 0.06-2.2323 +/- 0.13 mumol/g) and ketone body ratio remained at significantly higher values (0.26 to 0.68). After CLP, the liver lipoperoxide (LPO) concentration increased and glutathione (GSH) contents decreased significantly. When the septic rats were pretreated with methyl palmitate, both the LPO and GSH returned to the normal control level (62.69 +/- 1.7 to 44.62 +/- 2.12 and 159.85 +/- 9.7 to 222.27 +/- 11.34). It is concluded that the hepatic dysfunction is modulated at least to a greater extent by many of the toxic mediators released by the activated Kupffer cells during sepsis.
  相似文献
10.
Fifteen hybrid rats divided int0 3 groups (A, B and C) were fed for 250 days with distilled, 50 ppm and 80 ppm NaF water respectively. Before exsanguination, doubled tetracycline labelings were made on all of the experimental animals for bone dynamic study. The available data suggest that the primary mineralization and matrix synthesis in group B and C were significantly inhibited, indicating the toxic effects of high fluoride on osteoblasts. For a better understanding of the pathogenesis of osteos clerosis, osteomalacia and osteoporosis in endemic fluorosis, we wou.lld suggest that fluoride be used not only as a stimulator for bone formation as is being recommended in the treatment of postmeno pausal ostcoporosis accompanied somewhat by osteosclerosis, but also as an inhibitor for matrix synthesis and primary mineralization through its toxic effects on osteoblast. We believe that the pathology of fluorosis depends largely on the dose of fluoride intake.  相似文献
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