糖尿病酮症酸中毒患儿氧化应激状况及影响因素分析

Objective To investigate the oxidative stress state in children with diabetic ketoacidosis (DKA),and analyze whether any observed abnormalities were related to metabolic disturbances.Methods Four groups of subjects were studied,comprising with 22 patients with DKA(group 1),18 diabetic children with medium metabolic control,whose glycated hemoglobin(HbAlc) below 9%(group 2),22 children with poorly controlled diabetes,whose Hbal c above 9%(group 3),and 36 healthy control children(group 4).Malondialdehyde(MDA),nitrate oxidase(NO),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and metabolic parameters such as fasting blood glucose,HbAlc,blood gas analysis,serum ion,renal function of the subjects were measured.Results Mean serum MDA value was significantly higher in group 1 than in group 2(P ＜0.01) and group 4(P ＜0.01),but was not different from that in group 3 (P ＞0.05).Serum levels of NO were significantly higher in group 1,2 and 3 compared with group 4(P＜0.01).Serum levels of GSH-Px were significantly lower in group 1,2 and 3 than in group 4(P＜0.01).There were no significant differences of serum SOD value among the four groups(P＞0.05).Both serum MDA and NO values in the diabetic patients(groups 1 +2 +3) were positively related to HbAlc(r=0.375,P ＜0.01;r=0.250,P ＜0.05),and serum SOD values in the DKA patients were negatively related to HbAlc(r= -0.507,P＜0.05),there were not any significant relationship between the other oxidative markers and metabolic parameters.Conclusion There is an increase of oxidative stress and decrease of antioxygenic ability in DKA children,and these changes tend to correlate more with markers of diabetic imbalance than with parameters of acute metabolic disturbances of DKA.     

糖尿病酮症酸中毒患儿氧化应激状况及影响因素分析

Objective To investigate the oxidative stress state in children with diabetic ketoacidosis (DKA),and analyze whether any observed abnormalities were related to metabolic disturbances.Methods Four groups of subjects were studied,comprising with 22 patients with DKA(group 1),18 diabetic children with medium metabolic control,whose glycated hemoglobin(HbAlc) below 9%(group 2),22 children with poorly controlled diabetes,whose Hbal c above 9%(group 3),and 36 healthy control children(group 4).Malondialdehyde(MDA),nitrate oxidase(NO),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and metabolic parameters such as fasting blood glucose,HbAlc,blood gas analysis,serum ion,renal function of the subjects were measured.Results Mean serum MDA value was significantly higher in group 1 than in group 2(P ＜0.01) and group 4(P ＜0.01),but was not different from that in group 3 (P ＞0.05).Serum levels of NO were significantly higher in group 1,2 and 3 compared with group 4(P＜0.01).Serum levels of GSH-Px were significantly lower in group 1,2 and 3 than in group 4(P＜0.01).There were no significant differences of serum SOD value among the four groups(P＞0.05).Both serum MDA and NO values in the diabetic patients(groups 1 +2 +3) were positively related to HbAlc(r=0.375,P ＜0.01;r=0.250,P ＜0.05),and serum SOD values in the DKA patients were negatively related to HbAlc(r= -0.507,P＜0.05),there were not any significant relationship between the other oxidative markers and metabolic parameters.Conclusion There is an increase of oxidative stress and decrease of antioxygenic ability in DKA children,and these changes tend to correlate more with markers of diabetic imbalance than with parameters of acute metabolic disturbances of DKA.     

腹部穴位埋线配合葛根芩连汤对湿热困脾证2型糖尿病血糖控制情况观察

目的:观察腹部穴位埋线配合葛根芩连汤对湿热困脾证2型糖尿病(T2DM)血糖控制情况的影响。方法:选取湿热困脾证T2DM患者94例,随机分为观察组(n=47)与对照组(n=47)。对照组予西药治疗(西格列汀+二甲双胍),观察组于对照组基础上加用腹部穴位埋线配合葛根芩连汤治疗,均持续治疗3个月。统计两组治疗效果、不良反应发生情况及治疗前、治疗3个月后血糖[空腹血糖(FPG)、餐后2 h血糖(2h PG)、糖化血红蛋白(HbA1c)]、体质量指数(BMI)、腰围水平。结果:观察组治疗3个月后总有效率93. 62%(44/47)高于对照组70. 21%(33/47)(P 0. 05);两组治疗前FPG、2h PG、HbA1c、BMI、腰围水平对比,差异无统计学意义(P 0. 05);两组治疗3个月后FPG、2h PG、HbA1c、BMI、腰围水平均较治疗前降低(P 0. 05),且观察组上述指标水平低于对照组(P 0. 05);观察组不良反应发生率14. 89%(7/47)与对照组17. 02%(8/47)比较,差异无统计学意义(P 0. 05)。结论:应用腹部穴位埋线配合葛根芩连汤治疗湿热困脾证2型糖尿病患者可明显提高治疗效果,改善患者血糖控制情况,降低患者BMI及腰围,且安全性良好。     

小切口非超声乳化术治疗白内障临床观察

本人在2005～2006年采用小切口非超声乳化进行白内障摘除联合人工晶体植入术96例,报道如下。     

依据解剖学要点护理操作在眼科用药护理中的应用

目的 探讨依据解剖学要点的临床护理操作在眼科用药护理中的应用.方法 按照不同的护理方式将在我院进行治疗的眼科患者分为常规护理组(RC组)和基于解剖学要点的临床护理组(CN组),每组71例.两组患者一般资料差异无统计学意义(P＞0.05).比较RC组和CN组患者SF-36评分、负面情绪、护理满意度的差异性.结果 RC组患...     

高良姜活性部位的镇痛研究

[摘要] 目的 研究高良姜活性部位镇痛活性。方法 采用热板法、甩尾法、扭体法对以高良姜素、芹菜素组成的高良姜活性部位进行镇痛活性研究。结果 热板法试验中高良姜活性部位大、中剂量,甩尾法试验中、小剂量组,扭体法试验小剂量组以下与空白对照能明显提高小白鼠痛阈。结论 高良姜活性部位具有明显的镇痛作用。     

左金丸抗幽门螺杆菌感染的分子网络调控机制研究

目的基于网络药理学和生物信息学方法探究左金丸抗幽门螺杆菌(Hp)感染的分子网络调控机制。方法通过中药系统药理学数据库和分析平台(TCMSP)检索左金丸化学成分,筛选并预测其入血活性成分和作用靶点,检索疾病数据库中与Hp感染相关的作用靶点,构建左金丸成分靶点与疾病靶点的交互网络,获得左金丸抗Hp的特征性基因;通过DAVID6.8数据库对上述特征性基因进行GO功能富集和KEGG通路富集分析;利用Cytohubba筛选出左金丸抗Hp感染的关键靶点。结果通过TCMSP筛选、预测得到左金丸32个入血活性成分和197个作用靶点。GO分析共得到199条富集结果,其中生物过程包含炎症反应、RNA信号转录、信号传导等152条,细胞组分包含细胞核、细胞外基质、蛋白复合物等19条,分子功能包含细胞因子活性、DNA结合、ATP结合等28条。KEGG分析结果显示,Jak-STAT信号通路、T细胞受体信号通路、细胞周期信号通路、Wnt信号通路等65条通路与左金丸抗Hp感染密切相关。经Cytohubba筛选得到CXCL8、IL10、IL4、VEGFA、MMP9等10个关键基因。结论左金丸抗Hp感染具有多成分、多靶点、多通路协同作用的特点,可为其活性成分研究和抗Hp药效及机制研究提供依据。     

超高效液相色谱－蒸发光散射检测器法测定参松养心胶囊中人参皂苷Rg1和Re含量

目的：建立测定参松养心胶囊中人参皂苷Rg1和Re含量的超高效液相色谱－蒸发光散射检测器（UPLC－ELSD）法。方法采用Waters ACQUITY UPLC BEH C18柱（100 mm ×2.1 mm,1.7μm）为色谱柱,以乙腈－水为流动相(梯度洗脱),流速为0.45 mL/min,柱温为35℃,进样量为2μL,ACQUITY ELSD检测器的漂移管温度为50℃,气体流量为30.0 psi。结果参松养心胶囊中人参皂苷Rg1和Re进样量分别在0.09108~1.1358μg和0.08306~1.03825μg范围内与峰面积线性关系良好,平均加样回收率分别为99.01%和99.25%,RSD分别为0.50%和0.36%（ n=6）。结论与高效液相色谱（HPLC）法相比,UPLC法在不影响分离效果的情况下大大提高了参松养心胶囊中人参皂苷Rg1和Re的分离速度,同时减少了溶剂消耗。UPLC法可作为替代传统HPLC法的一种更方便、快捷、可靠的方法。     

糖尿病足患者给予预防性护理干预的效果探讨

目的探讨糖尿病足患者给予预防性护理干预的效果.方法将2010年1月~2011年2月收治的58例糖尿病患者随机分为两组.对照组给予常规临床护理,试验组患者给予预防性护理,比较半年后患者的知识了解情况和患者遵医行为.结果试验组患者经过预防性护理后,其足部溃疡发生少,患者对疾病知识了解更好,其自护行为得分更高,与对照组比较有显著差异,P<0.05.结论对糖尿病足患者给予预防性护理干预能够取得较好的效果,提高患者自我能力,减少糖尿病足的发生发展     

标准化操作流程对提高早产儿桡动脉穿刺采血成功率的效果观察

[目的]探讨早产儿桡动脉穿刺采血标准化操作流程对提高采血成功率的效果.[方法]早产儿72例(常规组)桡动脉采血按照常规的方法,77例(改良组)桡动脉采血按照通过品管圈质量管理活动改善后的标准化操作流程.[结果]应用早产儿桡动脉穿刺采血标准化操作流程后桡动脉一次穿刺成功率明显高于活动前,差异有统计学意义(P＜0.05).[结论]应用桡动脉穿刺采血标准化操作流程能提高早产儿桡动脉穿刺采血成功率,值得临床推广应用.