Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

蛇毒精氨酸酯酶Agkihpin上调人肝癌细胞株SMMC-7721中表皮钙黏素的表达研究

目的探讨蛇毒精氨酸酯酶Agkihpin对人肝癌SMMC-7721细胞株中表皮钙黏素(E-CD)表达的影响及Agkihpin抑制人肝癌SMMC-7721细胞的机制。方法采用不同浓度的Agkihpin处理肝癌细胞株72 h后,应用免疫细胞化学、Western Blotting和逆转录PCR(RT-PCR)法检测E-CD在SMMC-7721细胞中的表达。结果不同浓度Agkihpin作用SMMC-7721细胞72 h后E-CD表达均上升,差异有统计学意义(P<0.05),并呈现出一定的剂量依赖效应。Agkihpin可显著上调SMMC-7721细胞中E-CD表达。结论在人低分化肝癌SMMC-7721细胞株中,Agkihpin能促进E-CD的表达,因而可能抑制肝癌细胞的迁移和降低肝癌组织的恶性程度。     

Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

蛇毒精氨酸酯酶的研究与应用

精氨酸酯酶(Arginine Esterase)是蛇毒中的一类蛋白水解酶,因能专一性地水解精氨酸酯类化合物而得名.精氨酸酯酶广泛地存在于蝮亚科及蝰科蛇毒中.这类酶多数对凝血系统有重要作用,其抗凝血作用类似于哺乳动物凝血酶,与哺乳动物凝血酶不同之处是此酶在体内不能激活血浆中的Ⅷ因子,不能形成稳定的纤维蛋白凝块,仅能形成松散的纤维蛋白凝胶,易被纤溶酶分解掉,从而使血小板解聚,降低血黏度.由于多种精氨酸酯酶在体内具有明显的去纤、溶栓作用,从加世纪60年代开始,国内外已有多种蛇毒中的精氨酸酯酶用于临床的抗凝和溶栓.     

蛇毒精氨酸酯酶的研究与应用

精氨酸酯酶(ArginineEsterase)是蛇毒中的一类蛋白水解酶,因能专一性地水解精氨酸酯类化合物而得名。精氨酸酯酶广泛地存在于蝮亚科及蝰科蛇毒中。这类酶多数对凝血系统有重要作用,其抗凝血作用类似于哺乳动物凝血酶,与哺乳动物凝血酶不同之处是此酶在体内不能激活血浆中的Ⅷ因     

Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

Parkin基因启动子区-258T/G多态性与广西地区散发性帕金森病的相关性分析

Objective To investigate the association between the-258T/G polymorphism.in the pro-moter of parkin gene and the risk for sporadic parkinson's disease (SPD) in Guangxi Province, and in relationto the age of onset, of PD patients. Methods PCR-RFLP and sequence analysis were used to determine thegenotype of-258T/G polymorphism between all patients and healthy controls. Results The G allele was morecommon in patients than controls (55.20%:43.33% ,x2=6.898, P<0.05, OR=1.61, 95% CI: 1.13 ～2.30). The frequency of GG genotype was higher in patients than in controls (28.00 %: 18.33%, x2=7.159, P<0.05, OR=2.75, 95% CI : 1.31 ～ 5.77). The frequency of TG + GG genotype was higher in pa-tients than in controls (82.40%:68.33%, x2=6.551, P<0.05, OR=2.17.95%CI: 1.20 ～3.93). Afterbeing stratified by onset age, the frequencies of the G allele and GG genotype were significantly higher in pa-tients with onset age over 50 years than those in controls respectively. On the other hand, the frequency was notsignificantly different between the younger onset PD patients and the controls. Conclusion The parkin promot-er-258T/G polymorphism might be a risk factor for PD in Guangxi Province, and the G allele was increasedwith increasing age.     

浅谈实验教学的质量控制

实验教学在医学院校教学体系中曾一度处于被忽视的从属地位。但是,在全面提高教学质量以求学校生存和发展的新形势下,实验教学正逐渐受到重视。实验教学是高等医学院校教学体系的重要组成部分,它在培养医学生的实践能力、创新能力和综合素质方面具有其他教学环节不可替代的作用[1]。因此,加强实验教学的质量控制就成为大家的关注点。质量是指事物的内在素质及其优劣程度;而控制则是带目的性的因果联系,即产生原因系统对产生结果系统有目的的影响和干预[2]。实验教学是一个复杂的系统,影响其质量的因素很多,包括教学计划及其实施方案、实施过程、师资队伍素质与教学水平、学生素质、实验基本条件、教学环境质量等等。因此,实验教学的质量就存在较大的波动性。而实验教学的质量控制就是要通过科学的管理、监控、分析、评价手段,不断地改善影响质量的因素,及时纠正偏差,使实验教学向着预定目标发展。实验教学的质量控制要根据实验教学的运作规律进行。在全面质量管理思想中,质量的保证体现在各工作环节和工作项目标准的建立与管理控制的规范程度,全过程性是内部质量管理的一个基本理念[3]。实验教学存在一条运作主轴:教学计划→实验课程教学大纲→实验教学过程→学生学习质量。因此...     

早发型帕金森病DJ-1基因突变的分析

目的:分析广西地区早发型帕金森病(Parkinsion's disease,PD)患者及常染色体隐性遗传早发型帕金森病(autosomal recessive early-onset Parkinsion's disease,AREP)家系患者DJ-1基因外显子的突变特点,探讨DJ-1基因外显子的突变与广西地区PD关系.方法:应用聚合酶链式反应(PCR)、单链构象多态性(SSCP)及DNA测序等技术查找DJ-1基因缺失突变及点突变.结果:45例早发型散发性PD患者和12例分别来自5个常染色体隐性遗传早发型PD家系的DJ-1基因的2～7号外显子全部被成功扩增,未见大片段缺失.产物经SSCP方法和测序检测,未见点突变与缺失突变.结论:DJ-1基因的突变不是广西地区早发型PD患者的发病的危险因素.     

蛇毒精氨酸酯酶Agkihpin抑制人肝癌SMMC-7721细胞株MRP1表达

  目的   探讨蛇毒精氨酸酯酶Agkihpin对人肝癌细胞株SMMC-7721中多药耐药相关蛋白1(multidrug resistance associated protein 1, MRP1)表达的影响, 并阐明Agkihpin抑制人肝癌SMMC-7721细胞活力的机制。   方法   目的: 方法: 采用不同浓度的Agkihpin处理SMMC-7721细胞72 h后, 应用免疫细胞化学、Western blot和RT-PCR等方法检测MRP1在SMMC-7721细胞中的转录和表达。   结果   不同浓度Agkihpin作用SMMC-7721细胞72 h后MRP1表达均降低, 显示Agkihpin可显著下调SMMC-7721细胞中MRP1转录和表达(P < 0.05), 并呈现出一定的浓度依赖效应。   结论   Agkibpin能抑制人低分化肝癌细胞株SMMC-7721中MRP1的表达, 并呈一定程度的浓度依赖效应, 提示Agkihpin在一定程度上可用于提高肝癌细胞对化疗药物的敏感性。