兔脑 —氨基丁酸结合蛋白（受体）在非洲瓜蟾卵母细胞上的重建

Gurdon's Xenopus laevis oocyte translation system has been extensively employed to investigate the molecular biology of gamma-aminobutyric acid (GABA) receptors in the brains of embryonic chick, rat, etc. As GABA and its receptor may play a role in the pathogenesis of hepatic encephalopathy and a rabbit model of fulminant hepatitis has been established, we reconstituted the rabbit model of fulminant hepatitis has been established, we reconstituted the rabbit brain GABA binding protein (receptor) on Xenopus laevis oocytes to offer a base for further study of the pathological effects of GABA receptor at molecular level. In this report, total RNA was extracted from rabbit brain by guanidine hydrochloride method. The total RNA was then subjected to oligo-(dT)-cellulose affinity chromatography to isolate mRNA. All the RNA samples achieved a high purity with an average OD260/OD280 ratio of about 2.10. The clear bands of 18 S and 28S rRNA in electrophoresis implied that the RNA was not degraded and was therefore available for expression. After two days of incubation of the oocytes injected with mRNA, radioreceptor assay indicated that saturable and specifically displaceable GABA binding sites were implanted onto the oocyte membrane. This result led us to the conclusion that rabbit brain GABA binding protein (receptor) can be reconstituted by injecting exogenous mRNA inclusive of those encoding the receptor into Xenopus oocytes. However, the functional activity of the reconstituted receptor as a GABA gated chloride ion channel needs to be further characterized by patch clamping.     

豚鼠钩体病PDH模型血浆血栓素和前列腺环素测定的初步研究

豚鼠钩体病肺弥漫性出血(PDH)模型作为实验组,用体重相近的健康豚鼠作为对照组。实验组动物于感染钩体菌后38小时在局麻下作颈总动脉向心端插管采集血标本,用放免法测定血浆血栓素A_2(TXA_2)稳定代谢物TXB_2和前列腺环素(PGI_2)的稳定代谢物6—酮—PGF_1a的值。采血后砍     

兔脑γ-氨基丁酸结合蛋白(受体)在非洲爪蟾卵母细胞上的重建

作者采用盐酸胍法提取兔脑RNA,并经寡聚-(dT)-纤维素亲和层析分离获得完好的总mRNA,这种异源mRNA注入非洲爪蟾卵母细胞胞浆后经放射受体分析证实,γ-氨基丁酸结合蛋白(受体)能被重建于细胞膜上。该重建技术稳定可靠,为进一步进行γ-氨基丁酸受体的体外研究奠定了基础。     

兔脑γ-氨基丁酸结合蛋白(受体)在非洲爪蟾卵母细胞上的重建

非洲爪蟾卵母细胞翻译系统已被广泛用于胚鸡、大白鼠等动物脑GABA受体的分子生物学研究。鉴于GABA及其受体在肝性脑病中的作用和暴发性肝炎兔模型的建立,我们在卵母细胞上重建了兔脑GABA结合     

豚鼠钩体病PDH模型血浆血栓素和前列腺环素的测定

对7只钩体病肺瀰漫性出血(PDH)的豚鼠用放射免疫法测定动脉血血浆血栓素A_2和前列腺环素的稳定代谢产物TXB_2和6-酮-PGF_1a值发现:在肺出血明显期血浆TXB_2值明显升高(107.15±41.65 pg/ml),约为对照组值(54.05±12.93 pg/ml)的两倍(n=7,P<0.01);6-酮-PGF_2a值(67.97±16.89pg/ml,n=6)与对照值(98.06±40.63pg/ml,n=9)比较无显著性差异(P>0.1)。作者认为,TXA_2增多在豚鼠钩体病PDH的发病机理中可能起一定作用。