雷公藤单体对大鼠睾丸间质细胞及支持细胞毒性与其抗生育作用不相关

本文用体外培养的睾丸间质细胞和支持细胞筛选了２２种从雷公藤根、皮中分离、纯化的单体，并选择其中对两种细胞杀伤作用强、弱不同的化合物ＴＷ－９，ＴＷ－５，ＴＷ－２８和ＴＷ－２７进行抗生育试验以及体外杀精试验。结果发现对两种细胞毒性作用最弱的ＴＷ－１９对大鼠有明显的抗生育作用；而毒性作用较强的ＴＷ－５，ＴＷ－２８和最强的ＴＷ－２７则无明显的抗生育作用。然而这四种化合物的体外杀精强度则依次为：ＴＷ－２７，ＴＷ－５，ＴＷ－１９，ＴＷ－２８。与它们的抗生育作用不相关。     

Comparative Potency of Seven Compounds Isolated from TripterygiumWilfordii on Cultred Rat Leydig Cells and Sertoli Cells

The damaging effect of seven compounds isolated from Triptervgium Witfordii Hook. F, were tested on the rat Leydig cells and Sertoli cells in culture. The results showed that 10μg/ ml orthosphenic acid (TW2), 10μg/ ml 3β,22α-dihydroxy-△^12 ursen-30-oic acid(TW5), 5.0 μg/ ml 3β,22α -dihydroxy-△^12-oleanen-29-oieaeid(TW6), 5.0μg/ ml salaspermic acid(TW7), 0.5μg/ mt eelastrol(TW27) and 1.25μg/ml polpunonic acid (TW28) were all detrimental to Leydig cells and Sertoti eells. However, 30μg/ ml wilJbrlideA (TW1) did not affect the viability of Leydig cells and Sertoli cells significantly. The most toxic compounds was TW27, and the least, TW1. These results suggested that the -COOH at the C-20 site of these triterpenoids mat, be tbe funetional chemical group responsible for the damaging effect on the Leydig cells and Sertoli cells.