丹参素对大鼠肝星状细胞氨基末端蛋白激酶信号转导的影响

Objective To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1 β. Methods The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expres-sion and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen Ⅰ were detected by the quantitative immunocytochemical assay and ELISA. Results Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-115 (P < 0.05). Synthesis and secretion of Type β collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1 β was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu. Conelutions Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type β collagen, possibly via the repression of the JNK signal transduction.     

丹参素对IL-1β刺激的大鼠肝星状细胞JNK、NF-κB信号通道的影响

目的 观察丹参素对活化的大鼠肝星状细胞(HSC)氨基末端蛋白激酶(JNK)以及NF-κBP65表达的影响,探讨其抗肝纤维化作用的机制.方法 分离大鼠原代HSC,MTT法检测丹参素对细胞的生长抑制,免疫细胞化学法观察Ⅲ型胶原合成,ELISA法观察Ⅲ型胶原分泌.AO/EB荧光染色观察HSC凋亡形态学变化,Annexin-V-FITC/PI联合流式细胞仪检测HSC的凋亡率,Western blotting观察丹参素对IL-1β刺激的HSCs内P-IκBα、NF-κBP65和JNK蛋白的表达.结果 和结论丹参素可抑制活化的HSC增殖,降低胶原合成和分泌,促进HSC凋亡,并抑制IL-1β刺激的HSC中JNK的磷酸化及NF-κBp65的表达.     

丹参素对大鼠肝星状细胞氨基末端蛋白激酶信号转导的影响

目的 探讨丹参素抗肝纤维化作用的机制. 方法分离大鼠原代肝星状细胞(HSC),用0.0312、0.0625、0.1250、0.2500、0.5000、1.0000 mmol/I.不同浓度丹参素作用于HSC,四甲基偶氮唑黼法检测丹参素对HSC生长增殖的抑制作用;应用免疫细胞化学法观察Ⅰ型胶原合成,酶联免疫吸附法观察Ⅰ型胶原分泌.Western blot法观察丹参索对白细胞介素-1 β(IL-1 β)刺激的HSC内氨基末端蛋白激酶(JNK)蛋白及其磷酸化表达的影响.采用随机设计的方差分析,SNK-q进行统计学处理. 结果与0 mmol/L组比较,丹参素其他浓度组明显抑制了HSC增殖,并旱剂量依赖性.丹参素(0.0625～0.2500 mmol/L)对IL-1 β引起的HSC增殖具有明显的抑制作用,并呈剂量依赖性.0.25 mmol/L丹参素对正常培养的和经IL-1 β刺激的HSC作用24 h能下调Ⅰ型胶原的合成和分泌.IL-1 β能刺激ttSC中p-JNK的明显表达,丹参素能下凋亡IL-1 β诱导的ItSC中P-JNK的表达,但其对JNK表达水平没有明显影响. 结论丹参索可抑制正常传代培养的和经IL-1 β刺激的人鼠HSC增殖、Ⅰ型胶原合成与分泌,其机制可能与抑制JNK信号转导通路有关.     

自体骨髓移植治疗小儿单房性骨囊肿(附2例报告及文献复习)

目的比较儿童单房性骨囊肿的治疗机理及治疗方法。探讨移植自体骨髓治疗方法的优越性。方法 2例单房性骨囊肿位于肱骨近端 ,抽去囊液后注入从髂骨吸取的骨髓。并结合文献予以复习。结果平均用骨髓 3 0 ml左右 ,每个穿刺点骨髓有核细胞为 60 .0×10 9/ L左右 ,未见手术并发症发生。成人骨髓间质干细胞能分化成多种人体组织细胞是该方法治疗的理论基础。囊腔容积的大小、囊腔的个数以及囊腔能否被注满是影响治愈率的因素 ,囊腔小、单房性骨囊肿者治疗效果好。自体骨髓来源广泛、取材便利 ,大大降低了医疗费用 ,减轻了患者的经济负担。结论经皮囊腔内注射移植自体骨髓治疗单房性骨囊肿疗效满意 ,操作方便 ,患者痛苦小 ,治疗费用低     

某医院放射防护监督管理30年

目的 加强辐射源的监督管理,确保放射工作人员及公众的健康与安全.方法 通过总结放射防护工作,依据档案、日常监督管理资料.结果 1977～2006年来,“三建“放射场所预防性申报、审查、审批、验收,监测率100%;健康体检率96%～100%,个人剂量监测率97.47%.结论 应继续做好“五抓“,提高自主管理水平和管理效益,确保其健康、安全.     

丹参素对大鼠肝星状细胞氨基末端蛋白激酶信号转导的影响

Objective To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1 β. Methods The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expres-sion and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen Ⅰ were detected by the quantitative immunocytochemical assay and ELISA. Results Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-115 (P < 0.05). Synthesis and secretion of Type β collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1 β was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu. Conelutions Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type β collagen, possibly via the repression of the JNK signal transduction.     

丹参素对大鼠肝星状细胞氨基末端蛋白激酶信号转导的影响

Objective To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1 β. Methods The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expres-sion and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen Ⅰ were detected by the quantitative immunocytochemical assay and ELISA. Results Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-115 (P < 0.05). Synthesis and secretion of Type β collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1 β was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu. Conelutions Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type β collagen, possibly via the repression of the JNK signal transduction.     

丹参素对大鼠肝星状细胞氨基末端蛋白激酶信号转导的影响

Objective To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1 β. Methods The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expres-sion and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen Ⅰ were detected by the quantitative immunocytochemical assay and ELISA. Results Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-115 (P < 0.05). Synthesis and secretion of Type β collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1 β was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu. Conelutions Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type β collagen, possibly via the repression of the JNK signal transduction.     

丹参素对大鼠肝星状细胞氨基末端蛋白激酶信号转导的影响

Objective To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1 β. Methods The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expres-sion and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen Ⅰ were detected by the quantitative immunocytochemical assay and ELISA. Results Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-115 (P < 0.05). Synthesis and secretion of Type β collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1 β was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu. Conelutions Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type β collagen, possibly via the repression of the JNK signal transduction.