中华医学会肾脏病学会第一届全国中青年肾脏病学术会议纪要

中华医学会肾脏病学会第一届全国中青年肾脏病学术会议纪要姜傥李晓玫杨军陈香美中华医学会肾脏病学会第一届全国中青年肾脏病学术会议，于１９９５年１０月４～７日在北京召开。会议收到来自全国２８个省、市、自治区（除甘肃、青海和台湾以外）的论文５８７篇以及海外中...     

镍铬合金点式电解蚀刻后与粘结材料的粘结强度

本文用点式电解蚀刻技术对国产Ni—Cr合金处理来提高金属—粘结材料的粘结强度,并对该项技术进行了系统地研究。结果为:1.通过正交设计选出Ni—Cr合金点式电解蚀刻可采用的配方为5％H_2SO_4,350mA/cm~2,2—4分钟。2.点式电解蚀刻与传统电解蚀刻所获得的金属—树脂粘结强度无显著性差别。3.喷砂处理或应用偶联剂对点式电解蚀刻产生的粘结强度无显著影响。4.点式电解蚀刻后Ni—Cr合金与复合树脂获得较高的粘结强度,其中CHEMIACE粘结强度最高(平均27.92MPa),显著高于玻璃离子以及复合羧聚陶瓷粘结材料获得的粘结强度(p<0.01)。文中指出点式电解蚀刻可以代替传统池式电解蚀刻。并对点式电解蚀刻技木的优点和注意事项进行了阐述。     

三种基牙材料对铸造玻璃瓷冠碎裂影响的研究

目的:研究不同种类和强度基牙材料与瓷冠破折碎裂的内在关系.方法:选用计算机建立上颌中切牙的三维有限元模型,模拟陶瓷层、牙本质、核桩等结构.分析在天然齿基牙、钴铬合金基牙及复合树脂基牙情况下,铸造玻璃陶瓷全冠的瓷层内及残余牙本质的应力分布情况.结果:高弹性模量的钴铬合金能够降低瓷层及残余牙本质内的最大主应力和最小主应力峰值,防止瓷层破折碎裂;而弹性模量与牙本质相近的复合树脂桩降低应力峰值的作用不明显.结论:对于有牙髓活力的牙本质基牙,尽量保存牙髓活力后行全冠修复.对于行根管治疗术后的无髓牙,进行核桩冠修复时,采用高弹性模量的高熔合金,尽量避免使用弹性模量较小的复合树脂核.     

Role of angiotensin Ⅱ and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion

Objective To investigate the effect of angiotensin (Ang) Ⅱ and its Janns-activated kinase-2 (JAK2) signal pathway in transdifferentiation of renal tubular cells under the challenge of acute ischemic reperfusion injury.Methods Models of acute ischemic reperfusion injury were established and the level of local Ang Ⅱ ,a key element of renin-angiotensin system (RAS),in kidney was measured using radioimmunity technique.The expression of α-smooth muscle actin (α-SMA),a phenotype of mesenchymal cells,was detected by RT-PCR and inununohistochemistry methods.Renal tubule cells ( NRK-52E) were cultured with various concentration of Ang Ⅱ ,followed by blocking of PD123319,Ang U receptor 2 antagonist,and AG490,an inhibitor of JAK2 signal pathway.Results Ang 0 of kidney tissue increased immediately after acute ischemic-reperfusion injury,in time dependent fashion.Expression of α-SMA in renal tubule cells was found at 48 hours after ischemic-reperfusion injury and in NRK-52E cells treated by high concentration of Ang Ⅱ and was dose and time dependent.The peak of α-SMA expression was seen after 30 minute treatment at the dose of 10-9'mol/L,which was interrupted by both of PD123319 and AG490.Conclusions Transdifferentiarion of renal tubular epithelial cells occurs under acute ischemic- reperfusion injury.Local renin-angiotensin system may play a role in the transdifferentiation of TEC through AT2 receptor and its JAK2 signal pathway.     

Role of angiotensin Ⅱ and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion

Objective To investigate the effect of angiotensin (Ang) Ⅱ and its Janns-activated kinase-2 (JAK2) signal pathway in transdifferentiation of renal tubular cells under the challenge of acute ischemic reperfusion injury.Methods Models of acute ischemic reperfusion injury were established and the level of local Ang Ⅱ ,a key element of renin-angiotensin system (RAS),in kidney was measured using radioimmunity technique.The expression of α-smooth muscle actin (α-SMA),a phenotype of mesenchymal cells,was detected by RT-PCR and inununohistochemistry methods.Renal tubule cells ( NRK-52E) were cultured with various concentration of Ang Ⅱ ,followed by blocking of PD123319,Ang U receptor 2 antagonist,and AG490,an inhibitor of JAK2 signal pathway.Results Ang 0 of kidney tissue increased immediately after acute ischemic-reperfusion injury,in time dependent fashion.Expression of α-SMA in renal tubule cells was found at 48 hours after ischemic-reperfusion injury and in NRK-52E cells treated by high concentration of Ang Ⅱ and was dose and time dependent.The peak of α-SMA expression was seen after 30 minute treatment at the dose of 10-9'mol/L,which was interrupted by both of PD123319 and AG490.Conclusions Transdifferentiarion of renal tubular epithelial cells occurs under acute ischemic- reperfusion injury.Local renin-angiotensin system may play a role in the transdifferentiation of TEC through AT2 receptor and its JAK2 signal pathway.     

牙槽骨高度对垂直移动的影响

目的研究牙槽骨高度对垂直移动的影响.方法建立不同牙槽骨高度的上颌中切牙的三维有限元模型,利用SAP91软件计算牙周膜的应力.结果①随牙槽骨高度的降低,牙周膜的应力值增大,根尖部牙周膜的应力值增大明显;②在不同的牙槽骨高度时,牙槽嵴顶部的牙周膜是应力集中的部位.结论伴有牙槽骨吸收的患者,垂直运动所需的力值需更加精确地控制.     

HSP90在原发性肾病综合征发生和治疗中的意义

【目的】热休克蛋白90（HSP90）是糖皮质激素受体（GR）的伴侣蛋白，本研究探讨HSP90在原发性肾病综合征（INS）发生和糖皮质激素（GC）在治疗中的作用本质。【方法】以INS患者和GC敏感、抵抗的细胞株为研究对象。分析其HSP90、GR表达水平及HSP90的亚细胞分布；并采用HSP90特异性阻断剂研究HSP90对GC反应性的影响。【结果】与对照组比较，INS患者HSP90mRNA表达水平明显增高；而且INS患者中GC抵抗组HSP90 mRNA表达显著高于GC敏感组（P〈0．05）。深入研究发现对照组的HSP90主要分布在细胞浆中，核内仅有微量分布；INS患者的HSP90表达有所增加，并且GC抵抗组与敏感组比较，HSP90明显趋向细胞核内分布（P〈0．001）。用HSP90特异性阻断剂后发现激素敏感细胞株的GC反应性也显著下降（P〈0．01）。【结论】研究表明HSP90的异常表达及亚细胞分布变化可能是导致GC抵抗的重要机制，其一方面可能影响内源性GC对神经-内分泌-免疫网络的功能调节，进而影响机体免疫内环境的稳定，从而参与INS等免疫损伤性疾病的发病；另一方面则可能干预患者对外源性GC的治疗反应，影响临床疗效。     

肝纤维化时血清Ⅳ型胶原蛋白的临床意义

本试验采用一种新的检测方法——乳胶透射比浊法检测118例肝病患和20名正常人血清Ⅳ型胶原蛋白（Ⅳ-C）浓度及其他肝功能指标水平．以探讨血清Ⅳ-C浓度对不同肝脏疾病的临床诊断价值。     

环孢素致离体肾小管上皮细胞损伤时的氨基酸代谢及甘,丙氨酸的保…

目的 研究环孢素Ａ（ＣｓＡ）的肾毒性机制。方法 制备了体外ＣｓＡ致肾小管上皮细胞（ＴＥＣ）损伤模型，对ＴＥＣ内氨基酸组成、含量、ＡＴＰ水平及乳酸脱氢酶（ＬＤＨ）释放率等指标进行了一系列检测。结果 （１）经ＣｓＡ处理，ＴＥＣ内氨基酸水平没有明显豪华；（２）体外投入甘、丙氨酸对ＣｓＡ所致的损伤可起保护作用，其作用与投入时机及剂量有关，而与Ｎａ＾＋－Ｋ＾＋－ＡＴＰ酶的抑制无直接联系。结论 ＣｓＡ肾中毒的