Measuring Psychological Inflexibility of Suicidal Thoughts: The Acceptance and Action Questionnaire for Suicidal Ideation (AAQ-SI)

Cognitive Therapy and Research - Despite interest in psychological inflexibility as a marker of suicide risk, no measure of psychological inflexibility specific to SI exists. The present study...     

Medizinstudierende als Helfende in der Pandemie

Die Anaesthesiologie - Die COVID-19-Pandemie hat das deutsche Gesundheitssystem vor enorme Herausforderungen gestellt und den Bedarf an Strategien zu Rekrutierung, Schulung und Einsatzplanung von...     

Patterns in metabolite profile are associated with risk of more aggressive prostate cancer: A prospective study of 3,057 matched case–control sets from EPIC

Metabolomics may reveal novel insights into the etiology of prostate cancer, for which few risk factors are established. We investigated the association between patterns in baseline plasma metabolite profile and subsequent prostate cancer risk, using data from 3,057 matched case–control sets from the European Prospective Investigation into Cancer and Nutrition (EPIC). We measured 119 metabolite concentrations in plasma samples, collected on average 9.4 years before diagnosis, by mass spectrometry (AbsoluteIDQ p180 Kit, Biocrates Life Sciences AG). Metabolite patterns were identified using treelet transform, a statistical method for identification of groups of correlated metabolites. Associations of metabolite patterns with prostate cancer risk (OR_1SD) were estimated by conditional logistic regression. Supplementary analyses were conducted for metabolite patterns derived using principal component analysis and for individual metabolites. Men with metabolite profiles characterized by higher concentrations of either phosphatidylcholines or hydroxysphingomyelins (OR_1SD = 0.77, 95% confidence interval 0.66–0.89), acylcarnitines C18:1 and C18:2, glutamate, ornithine and taurine (OR_1SD = 0.72, 0.57–0.90), or lysophosphatidylcholines (OR_1SD = 0.81, 0.69–0.95) had lower risk of advanced stage prostate cancer at diagnosis, with no evidence of heterogeneity by follow-up time. Similar associations were observed for the two former patterns with aggressive disease risk (the more aggressive subset of advanced stage), while the latter pattern was inversely related to risk of prostate cancer death (OR_1SD = 0.77, 0.61–0.96). No associations were observed for prostate cancer overall or less aggressive tumor subtypes. In conclusion, metabolite patterns may be related to lower risk of more aggressive prostate tumors and prostate cancer death, and might be relevant to etiology of advanced stage prostate cancer.     

Developmental Neurotoxicity of Domoic Acid: Evidence for a Critical Window of Exposure

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Harmful algal blooms are a growing worldwide problem.1 Toxins produced by some of these algae, including the neurotoxin domoic acid (DomA), may reach humans through contaminated seafood consumption.2^,3 Because acute high-level exposure may cause amnesic shellfish poisoning,4 countries around the world limit DomA to 20μg/g of shellfish tissue.5^,6^,7 However, relatively little is known about the health effects of chronic low-level exposure such as that experienced by people who regularly eat shellfish.8^,9^,10 In a recent study11 in Environmental Health Perspectives investigators based at the Woods Hole Oceanographic Institution (WHOI) in Massachusetts analyzed the developmental neurotoxic effects of DomA in zebrafish to help fill this gap.Open in a separate windowDomoic acid is produced by algal species including members of the Pseudo-nitzschia genus (shown). It causes amnesic shellfish poisoning, a potentially fatal illness that can strike people who eat contaminated seafood, such as clams, mussels, and crab. The disease was only discovered in 1987.3 Image: Pseudo-nitzschia: Vera Trainer/NOAA; razor clams: © iStockphoto/jack looney.The researchers exposed zebrafish embryos and larvae to DomA doses that were 3- to 260-fold lower than exposures tested in earlier studies.12^,13 Even the lowest nominal dose of 0.09ng during a defined developmental window caused behavioral deficits in the larvae. The researchers causally linked these deficits to disrupted myelination processes and altered gene expression.Zebrafish have distinct advantages as a model organism. Embryos are transparent during early development, and their nervous system structures are similar to those of humans. However, in zebrafish these structures develop externally rather than hidden inside a uterus. Thus, real-time imaging can reveal changes in labeled cells of interest during very early stages of development.Instead of the usual method of adding the agent of interest to the fish tanks, the researchers used microinjection into a cardinal vein to deliver a single dose of 0.09–0.18ng of DomA to the embryos and larvae. They administered doses at specific developmental periods between 1 and 4 days postfertilization (dpf). “Microinjection ensured that the desired dose reached the embryo and let us precisely time exposures throughout development to home in on a critical window,” says first author Jennifer M. Panlilio, who performed the research as a doctoral student in a joint program between WHOI and Massachusetts Institute of Technology.Following the injection, fluorescence time-lapse microscopy was used to track the movement of specialized cells in the spinal cord and the formation of protective myelin sheaths around axons, the part of the neuron that transmits electrical signals. Larval RNA was sequenced at 3 and 7 dpf, and myelin structure was assessed at 5–7 dpf. At 7 dpf, the researchers measured the larvae’s startle behavior in response to acoustic/vibrational stimuli. Well-known neural circuits and cell types drive this behavior.14^,15Exposure to 0.09ng DomA at 2 dpf had effects that were not observed at 1 or 4 dpf. It reduced the expression of genes required for maintaining axon and myelin structure, it produced structural deficits in myelin sheaths, and it delayed and changed typical motion features of the larvae’s startle response. However, it had no appreciable effects on mortality or gross morphology.“Our novel finding is a narrow critical window of development when DomA exposure disrupts the initial myelination of axons,” says Panlilio. “This is a potential molecular basis for an observable behavior, which provides an important functional end point for future research.” Even if the end point is similar in other organisms, she adds, the critical window may be different. The myelination process in humans, for example, starts in utero and continues throughout adolescence.For Rebekah Petroff, who was not involved in the new study, the results are consistent with observations in rodents,16^,17^,18^,19 marine mammals,20^,21 and nonhuman primates.22^,23 Petroff, a postdoctoral fellow at the University of Michigan, has studied DomA neurotoxicity in adult crab-eating macaques after low-level exposure.“Disrupted myelination pathways are a plausible mechanism for developmental end points that have been observed consistently across species,” says Petroff. “However, it will be difficult to translate how important these effects are until we know more about human exposure levels.” For example, the DomA exposure of fetuses and infants whose mothers consume contaminated shellfish is currently unknown.Jennifer Freeman, an associate professor of toxicology at Purdue University, appreciates the study’s precise targeting of different developmental stages. “I think we need to do more of that in developmental toxicology,” says Freeman, who also was not involved in the project. “If you don’t capture the susceptible period, you may completely miss an adverse health outcome.”Freeman finds the alignment of multiple pieces of evidence for the critical window of 2 dpf—namely, structural imaging, gene expression analysis, and functional outcome—compelling and considers it critical for regulating other environmental chemicals.Although researchers have identified several algal genes that produce DomA,24 we have only a limited understanding of the environmental stressors that trigger production of the toxin.25 Rising sea surface temperatures are predicted to increase the frequency of harmful algal blooms, including those with DomA-producing Pseudo-nitzschia species.26 DomA may persist in shellfish tissue long after the blooms dissipate, although substantial between- and within-species variation complicates predictions.27 “It’s a complex problem that’s challenging but important to regulate,” concludes Petroff.