全文获取类型
收费全文 | 7956篇 |
免费 | 818篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 47篇 |
儿科学 | 202篇 |
妇产科学 | 140篇 |
基础医学 | 1144篇 |
口腔科学 | 203篇 |
临床医学 | 1057篇 |
内科学 | 1935篇 |
皮肤病学 | 169篇 |
神经病学 | 953篇 |
特种医学 | 202篇 |
外科学 | 731篇 |
综合类 | 196篇 |
一般理论 | 2篇 |
预防医学 | 754篇 |
眼科学 | 188篇 |
药学 | 461篇 |
中国医学 | 2篇 |
肿瘤学 | 398篇 |
出版年
2021年 | 109篇 |
2020年 | 87篇 |
2019年 | 135篇 |
2018年 | 156篇 |
2017年 | 99篇 |
2016年 | 88篇 |
2015年 | 126篇 |
2014年 | 207篇 |
2013年 | 247篇 |
2012年 | 404篇 |
2011年 | 374篇 |
2010年 | 194篇 |
2009年 | 178篇 |
2008年 | 308篇 |
2007年 | 351篇 |
2006年 | 335篇 |
2005年 | 346篇 |
2004年 | 318篇 |
2003年 | 275篇 |
2002年 | 326篇 |
2001年 | 301篇 |
2000年 | 271篇 |
1999年 | 243篇 |
1998年 | 93篇 |
1997年 | 68篇 |
1996年 | 67篇 |
1995年 | 79篇 |
1994年 | 62篇 |
1993年 | 58篇 |
1992年 | 194篇 |
1991年 | 179篇 |
1990年 | 181篇 |
1989年 | 163篇 |
1988年 | 173篇 |
1987年 | 188篇 |
1986年 | 177篇 |
1985年 | 156篇 |
1984年 | 122篇 |
1983年 | 88篇 |
1982年 | 55篇 |
1981年 | 58篇 |
1980年 | 59篇 |
1979年 | 91篇 |
1978年 | 88篇 |
1977年 | 68篇 |
1975年 | 58篇 |
1974年 | 76篇 |
1973年 | 54篇 |
1972年 | 56篇 |
1971年 | 60篇 |
排序方式: 共有8784条查询结果,搜索用时 15 毫秒
1.
2.
Non‐melanoma skin cancer frequently results from chronic exposure to ultraviolet (UV) irradiation. UV‐induced DNA damage activates cell cycle arrest checkpoints through degradation of the cyclin‐dependent kinase activators, the cell division cycle 25 (CDC25) phosphatases. We previously reported increased CDC25A in nonmelanoma skin cancer, but CDC25B and CDC25C had not been previously examined. Consequently, we hypothesized that increased expression of CDC25B and CDC25C increases tumor cell proliferation and skin tumor growth. We found that CDC25B and CDC25C were increased in mouse and human skin cancers. CDC25B was primarily cytoplasmic in skin and skin tumors and was significantly increased in the squamous cell carcinoma (SCC), while CDC25C was mostly nuclear in the skin, with an increased cytoplasmic signal in the premalignant and malignant tumors. Surprisingly, forced expression of CDC25B or CDC25C in cultured SCC cells did not affect proliferation, but instead suppressed apoptosis, while CDC25C silencing increased apoptosis without impacting proliferation. Targeting CDC25C to the nucleus via mutation of its nuclear export sequence, however, increased proliferation in SCC cells. Overexpression of CDC25C in the nuclear compartment did not hinder the ability of CDC25C to suppress apoptosis, neither did mutation of sites necessary for its interaction with 14‐3‐3 proteins. Analysis of apoptotic signaling pathways revealed that CDC25C increased activating phosphorylation of Akt on Ser473, increased inhibitory phosphorylation of proapoptotic BAD on Ser136, and increased the survival protein Survivin. Silencing of CDC25C significantly reduced Survivin levels. Taken together, these data suggest that increased expression of CDC25B or CDC25C are mechanisms by which skin cancers evade apoptotic cell death. 相似文献
3.
James H. Holmes Michael J. Schurr Booker T. King Kevin Foster Lee D. Faucher Mary A. Lokuta Allen R. Comer Peggy J. Rooney Kelly F. Barbeau Stuart T. Mohoney Angela L.F. Gibson B. Lynn Allen-Hoffmann 《Burns : journal of the International Society for Burn Injuries》2019,45(8):1749-1758
ObjectiveThis open-label, controlled, randomized study assessed the safety, tolerability, and efficacy of StrataGraft tissue compared to autograft in the treatment of deep partial-thickness (DPT) burns.MethodsThirty subjects with DPT thermal burns (3%–43% total body surface area) were treated with StrataGraft tissue as follows: cohort 1, ≤220 cm2 refrigerated tissue; cohort 2, ≤440 cm2 refrigerated tissue; and cohort 3, ≤440 cm2 cryopreserved tissue. On each subject, two comparable areas of DPT burn were randomized to receive StrataGraft tissue or autograft. Coprimary end points were the percent area of the StrataGraft tissue treatment site undergoing salvage autografting by Day 28 and wound closure of treatment sites by 3 months.ResultsBy Day 28, no StrataGraft tissue treatment sites underwent autografting. By 3 months, 93% and 100% of the StrataGraft tissue and autograft treatment sites achieved complete wound closure, respectively. No significant differences in observer total and overall opinion POSAS scores between StrataGraft tissue and autograft treatment sites were observed at any timepoint. The most common adverse event was pruritus (17%).ConclusionsStrataGraft tissue treatment of DPT thermal burns reduced the need for autograft, resulted in wound closure and treatment-site cosmesis comparable to that of autograft, and was well tolerated. 相似文献
4.
Jun-Yu Zhang Tao-Hui Liu Ye He Han-Qing Pan Wen-Hua Zhang Xiao-Ping Yin Xiao-Li Tian Bao-Ming Li Xiao-Dong Wang Andrew Holmes Ti-Fei Yuan Bing-Xing Pan 《Neuropsychopharmacology》2019,85(3):189-201
Background
Chronic stress exposure increases the risk of developing various neuropsychiatric illnesses. The behavioral sequelae of stress correlate with dendritic hypertrophy and glutamate-related synaptic remodeling at basolateral amygdala projection neurons (BLA PNs). Yet, though BLA PNs are functionally heterogeneous with diverse corticolimbic targets, it remains unclear whether stress differentially impacts specific output circuits.Methods
Confocal imaging was used to reconstruct the morphology of mouse BLA PNs with the aid of retrograde tracing and biocytin staining. The synaptic activity in these neurons was measured with in vitro electrophysiology, and anxiety-like behavior of the mice was assessed with the elevated plus maze and open field test.Results
Chronic restraint stress (CRS) produced dendritic hypertrophy across mouse BLA PNs, regardless of whether they did (BLA→dorsomedial prefrontal cortex [dmPFC]) or did not (BLA?dmPFC) target dmPFC. However, CRS increased the size of dendritic spine heads and the number of mature, mushroom-shaped spines only in BLA?dmPFC PNs, sparing neighboring BLA→dmPFC PNs. Moreover, the excitatory glutamatergic transmission was also selectively increased in BLA?dmPFC PNs, and this effect correlated with CRS-induced increases in anxiety-like behavior. Segregating BLA?dmPFC PNs based on their targeting of ventral hippocampus (BLA→ventral hippocampus) or nucleus accumbens (BLA→nucleus accumbens) revealed that CRS increased spine density and glutamatergic signaling in BLA→ventral hippocampus PNs in a manner that correlated with anxiety-like behavior.Conclusions
Chronic stress caused BLA PN neuronal remodeling with a previously unrecognized degree of circuit specificity, offering new insight into the pathophysiological basis of depression, anxiety disorders, and other stress-related conditions. 相似文献5.
6.
7.
8.
9.
10.