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排序方式: 共有806条查询结果,搜索用时 15 毫秒
1.
Alessandro Beneduce MD Cristina Capogrosso MD Francesco Moroni MD Francesco Ancona MD Giulio Falasconi MD Luigi Pannone MD Stefano Stella MD Giacomo Ingallina MD Francesco Melillo MD Marco Bruno Ancona MD Vittorio Romano RT Anna Palmisano MD Azeem Latib MD Antonio Colombo MD Matteo Montorfano MD Antonio Esposito MD Eustachio Agricola MD 《Echocardiography (Mount Kisco, N.Y.)》2020,37(12):2071-2081
2.
Isolation and characterization of murine cell surface components. I. Purification of milligram quantities of Thy-1.1 总被引:1,自引:0,他引:1 下载免费PDF全文
The Thy-l.1 molecule was isolated from the BW5147 murine lymphoblastoid cell line. The initial step in purification was the preparation of a crude plasma membrane fraction followed by acetone precipitation. The acetone pellet was solubilized using deoxycholate (DOC) and Thy-1.1 was purified by use of a Lens culinaris lectin affinity column and an AcA-34 gel filtration column. The purified glycoprotein with Thy-1.1 activity had a mol wt of approximately 25,000 daltons. The isolation of this molecule was effected by detecting Thy-I activity utilizing rabbit anti- mouse brain serum tested on rat thymocytes. Congenic anti-Thy-l.1 serum was ineffective in detecting Thy-l.1 after DOC solubilization. An antiserum prepared in rabbits to the purified Thy-1.1 was found to be cytotoxic to mouse and rat thymocytes. The cytotoxic activity of this antisera could be completely absorbed with AKR/Jax brain and thymus but was not absorbed by liver. In addition, AKR/Jax thymocytes totally absorbed all cytotoxic activity of the rabbit anti-purified Thy-1 serum for BW5147 cells suggesting that the cell line shares identical specificities with normal thymocytes. The purified Thy-1.1 molecule was able to totally absorb the cytotoxic activity of mouse congenic anti-Thy-1. These studies serve as a model for the isolation of other murine lymphoid cell surface components in quantities for detailed structural and functional analysis. 相似文献
3.
Radiography on wheels arrives to nursing homes – an economic assessment of a new health care technology in southern Sweden 下载免费PDF全文
4.
We have found that the addition of 10 mM inorganic phosphate to DHA in CPD-adenine maintains ATP levels at normal or higher than normal values for six weeks of storage. 2,3-DPG values are slightly lowered by the extra phosphate, but are still maintained at approximately half normal for four weeks by the DHA. The addition of a higher phosphate concentration, 20 mM, to DHA produced lower levels of ATP and 2,3-DPG than those observed with 10 mM phosphate, although both levels were better than in the CPD-adenine control. pH values in this experiment were lowest in the three preservatives containing DHA, probably indicating increased lactate production due to metabolism of this triose sugar, in addition to dextrose present in CPD. 相似文献
5.
C. Warren Olanow MD Raymond T. Bartus PhD Laura A. Volpicelli‐Daley PhD Jeffrey H. Kordower PhD 《Movement disorders》2015,30(13):1715-1724
Trophic factors show great promise in laboratory studies as potential therapies for PD. However, multiple double‐blind, clinical trials have failed to show benefits in comparison to a placebo control. This article will review the scientific rationale for testing trophic factors in PD, the results of the different clinical trials that have been performed to date, and the possible explanations for these failed outcomes. We will also consider future directions and the likelihood that trophic factors will become a viable therapy for patients with PD. © 2015 International Parkinson and Movement Disorder Society 相似文献
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Measuring health care workers’ perceptions of what constitutes a compassionate organisation culture and working environment: Findings from a quantitative feasibility survey 下载免费PDF全文
Robert McSherry PhD RGN DipN B.Sc. MSc PGCE RT FHEC FFNRRCSI NTF Paddy Pearce RGN B.Sc. MSc 《Journal of nursing management》2018,26(2):127-139
10.
Angiography vs transesophageal echocardiography‐guided patent foramen ovale closure: A propensity score matched analysis of a two‐center registry 下载免费PDF全文