近交系胎鼠中脑多巴胺能神经元体外定向分化的初步研究

目的体外培养近交系大鼠胚胎腹侧中脑前体细胞(VMP)并诱导其分化为多巴胺能神经元(DN),为研究DN定向分化的分子机制提供细胞模型。方法取材胎龄11d的近交系大鼠胚胎VMP,体外用碱性成纤维细胞生长因子(bFGF)增殖培养7d后换用L-抗坏血酸-2-磷酸酯倍半镁盐(AA-2P)诱导分化为DN,随后进行免疫荧光染色鉴定。结果细胞总数扩增49.76倍,免疫荧光染色显示β-TubulinⅢ阳性的神经元中(71.33±20.42)%为TH阳性的DN,后者占细胞总数的(24.85±12.85)%。结论近交系大鼠VMP经体外原代培养能够得到较高比例的DN,可作为深入研究DN定向分化分子机制的细胞模型。     

骨髓基质干细胞向神经元样细胞分化前后BMP4基因差异表达的初步研究

目的初步探讨骨髓基质干细胞（BMSC）向神经元样细胞分化前后骨形态发生蛋白4（BMP4）基因的表达变化情况。方法体外培养大鼠BMSC,用碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）对其进行诱导,分别于诱导前和诱导后第7天行基因芯片检测BMP4基因表达水平,并采用实时定量PCR进行验证。结果在BMSC向神经元样细胞诱导分化后的芯片表达谱中,BMP4基因的表达明显上调,且实时定量PCR亦证实此结果。结论在BMSC向神经元样细胞的分化过程中,BMP4基因可能发挥着重要的作用。     

骨髓间充质干细胞促进中脑前体细胞增殖及定向分化的实验研究

目的 观察BMSCs对胚胎腹侧中脑前体细胞(VMP)体外扩增和定向分化的影响,并分析其可能的营养机制.方法 分别取胎龄11d大鼠胚胎VMP、成年大鼠BMSCs进行体外培养,并建立二者的共培养体系.体外扩增7 d的VMP分为对照组、BMSCs分化液组、BMSCs+VMP共培养分化液组,分别加入普通分化液、BMSCs分化液、BMSCs+VMP共培养分化液进行诱导分化.观察细胞生长情况.分化期末行免疫荧光染色,分析比较3组细胞中酪氨酸羟化酶(TH)阳性细胞占总细胞数的比例.结果诱导分化7 d后,对照组、BMSCs分化液组和BMSCs+VMP共培养分化液组中细胞数分别较培养前扩增(44.13±4.75)倍、(60.63±5.25)倍、(64.00±7.63)倍,TH阳性细胞比例分别为(18.76±5.20)%、(23.49±4.10)%、(28.08±5.42)%,比较差异有统计学意义(P<0.05).结论 BMSCs能够通过分泌营养因子有效促进VMP增殖并定向分化为多巴胺能神经元.     

骨髓基质干细胞向神经元样细胞诱导分化前后相关基因表达谱的变化

背景：一系列研究发现,骨髓基质干细胞可在体外经人工诱导分化为神经元样细胞,有望用以移植治疗神经退行性疾病,但是其分化的分子机制尚不明了。 目的：观察骨髓基质干细胞向神经元样细胞分化前后基因表达谱的变化。 设计、时间及地点：观察性实验,于2006-12/2007-12在苏州大学附属第二医院神经外科脑肿瘤研究室完成。 材料：健康成年近交系Wistar大鼠,体质量约300 g。 方法：体外培养和纯化大鼠骨髓基质干细胞,用碱性成纤维细胞生长因子和表皮生长因子对其进行诱导,分别于诱导前和诱导后第7天抽提RNA后利用Affymetrix基因芯片检测分析诱导前后差异基因表达谱,并有选择地对其中的一些重要基因进行实时定量PCR验证。 主要观察指标：①骨髓基质干细胞的培养及诱导分化。②诱导分化后神经细胞的鉴定。③诱导分化前后基因芯片的检测。④基因芯片结果的分析。⑤实时定量PCR的验证。 结果：骨髓基质干细胞经诱导后形态上呈神经元样细胞改变,免疫荧光证实β-TubulinⅢ染色后阳性率为(69.04±5.63)%。基因芯片共发现差异基因215条,其中上调基因125条,下调基因90条。明确与神经元发育密切相关的基因有13条,其中上调7条,下调6条。挑选重要的4条基因Bmp4、Robo2、Numb、Enc1进行实时定量PCR验证,其结果与基因芯片结果相符。 结论：骨髓基质干细胞在体外诱导分化为神经元样细胞的相关基因表达谱中,有一些明确与神经元分化密切相关的基因存在差异表达,深入研究这些基因可能为基因工程改造骨髓基质干细胞奠定基础。     

脑胶质瘤卒中误诊为急性脑出血临床分析

目的探讨脑胶质瘤卒中误诊的原因、临床表现及诊治方法。方法回顾性分析11例胶质瘤卒中病例的临床资料,病人初诊时误诊为脑出血,经手术和病理证实为脑胶质瘤。结果肿瘤大部分切除7例,部分切除4例。术后病理示间变性星形细胞瘤5例,多彤性胶质母细胞瘤3例,少突胶质细胞瘤2例,室管膜瘤1例。术后8例随访3—36个月,复发6例,再次手术4例。小组共死广6例,生存时间超过3年4例,失访1例。结论脑胶质瘤卒中起病急,初诊时易误诊为急性脑出血,CT增强扫描或MRJ检查有助于鉴别诊断。早期手术有利于明确诊断,提高病人生存率。     

骨髓间充质干细胞促进中脑前体细胞增殖及定向分化的实验研究

Objective To explore the potential neurotrophic effect of bone marrow-derived mesenchymal stem cells (BMSCs) on the proliferation and differentiation of ventral mesencephalic precursors (VMPs).Methods VMPs from E 11 inbred rat embryos and BMSCs from adult rats were cultured in 2 separate groups.Moreover,a co-cultured group was also established.Afterwards,the 3 different differentiation mediums obtained from these 3 defined groups were used to influence the differentiation procedures of normal VMPs that had amplification in vitro for 7 d.The growth of cells was observed;immunocytochemical staining was performed on these cells at the late differentiation phase.Relative yields of TH+ cells were calculated and compared.Results Seven d after the inducing differentiation,the total cell numbers multiplied about 44.13±4.75,60.63±5.25 and 64.00±7.63 folds in the VMP control group,the BMSCs group and the VMP+BMSCs co-cultured group,respectively,as compared with those before the differentiation.Correspondingly, the ratios of TH+ cells in the total cell population were (18.76±5.20)%, (23.49±4.10)% and (28.08±5.42)% in the VMP control group,the BMSCs group and the VMP+BMSCs co-cultured group,respectively; significant differences betwwen each 2 groups were found (P<0.05).Conclusion BMSCs can secret nutritional factors to promote the proliferation and committed differentiation of VMPs into dopaminergic neurons.     

水分离技术在治疗高血压脑出血中的应用

目的：探讨水分离技术在经侧裂岛叶手术治疗高血压脑出血中的应用。方法回顾性分析13例高血压基底核脑出血病人的临床资料,术中应用水分离技术解剖侧裂和清除血肿。结果10例血肿完全清除,3例血肿清除大于90%。13例病人随访3个月,日常生活能力分级为：l 级2例,2级7例,3级3例,4级1例。结论在经侧裂岛叶清除高血压基底核脑出血中使用水分离技术有助于解剖侧裂和清除血肿。     

CT定位微创治疗高血压脑出血患者的有效性分析

目的:探索CT定位微创疗法治疗高血压脑出血患者的疗效.方法:选取苏州市吴中人民医院神经外科2017年1月—2020年12月收诊的高血压脑出血患者80例,按照随机数字法分为对照组和观察组,各40例,对照组实施开颅血肿清除术,观察组实施C T定位下的微创血肿清除术,比较两组的术后并发症发生率、美国国立卫生研究院卒中量表(N...     

骨髓间充质干细胞促进中脑前体细胞增殖及定向分化的实验研究

Objective To explore the potential neurotrophic effect of bone marrow-derived mesenchymal stem cells (BMSCs) on the proliferation and differentiation of ventral mesencephalic precursors (VMPs).Methods VMPs from E 11 inbred rat embryos and BMSCs from adult rats were cultured in 2 separate groups.Moreover,a co-cultured group was also established.Afterwards,the 3 different differentiation mediums obtained from these 3 defined groups were used to influence the differentiation procedures of normal VMPs that had amplification in vitro for 7 d.The growth of cells was observed;immunocytochemical staining was performed on these cells at the late differentiation phase.Relative yields of TH+ cells were calculated and compared.Results Seven d after the inducing differentiation,the total cell numbers multiplied about 44.13±4.75,60.63±5.25 and 64.00±7.63 folds in the VMP control group,the BMSCs group and the VMP+BMSCs co-cultured group,respectively,as compared with those before the differentiation.Correspondingly, the ratios of TH+ cells in the total cell population were (18.76±5.20)%, (23.49±4.10)% and (28.08±5.42)% in the VMP control group,the BMSCs group and the VMP+BMSCs co-cultured group,respectively; significant differences betwwen each 2 groups were found (P<0.05).Conclusion BMSCs can secret nutritional factors to promote the proliferation and committed differentiation of VMPs into dopaminergic neurons.