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排序方式: 共有484条查询结果,搜索用时 62 毫秒
1.
2.
Wan Beom Park Ranawaka A.P.M. Perera Pyoeng Gyun Choe Eric H.Y. Lau Seong Jin Choi June Young Chun Hong Sang Oh Kyoung-Ho Song Ji Hwan Bang Eu Suk Kim Hong Bin Kim Sang Won Park Nam Joong Kim Leo Lit Man Poon Malik Peiris Myoung-don Oh 《Emerging infectious diseases》2015,21(12):2186-2189
We investigated the kinetics of serologic responses to Middle East respiratory syndrome coronavirus (MERS-CoV) infection by using virus neutralization and MERS-CoV S1 IgG ELISA tests. In most patients, robust antibody responses developed by the third week of illness. Delayed antibody responses with the neutralization test were associated with more severe disease. 相似文献
3.
Shamsudeen F. Fagbo Leila Skakni Daniel K.W. Chu Musa A. Garbati Mercy Joseph Malik Peiris Ahmed M. Hakawi 《Emerging infectious diseases》2015,21(11):1981-1988
We investigated an outbreak of Middle East respiratory syndrome (MERS) at King
Fahad Medical City (KFMC), Riyadh, Saudi Arabia, during March 29–May 21,
2014. This outbreak involved 45 patients: 8 infected outside KFMC, 13 long-term
patients at KFMC, 23 health care workers, and 1 who had an indeterminate source
of infection. Sequences of full-length MERS coronavirus (MERS-CoV) from 10
patients and a partial sequence of MERS-CoV from another patient, when compared
with other MERS-CoV sequences, demonstrated that this outbreak was part of a
larger outbreak that affected multiple health care facilities in Riyadh and
possibly arose from a single zoonotic transmission event that occurred in
December 2013 (95% highest posterior density interval November 8,
2013–February 10, 2014). This finding suggested continued health
care–associated transmission for 5 months. Molecular epidemiology
documented multiple external introductions in a seemingly contiguous outbreak
and helped support or refute transmission pathways suspected through
epidemiologic investigation. 相似文献
4.
Wen Da Guan Chris Ka Pun Mok Zi Lin Chen Li Qiang Feng Zheng Tu Li Ji Cheng Huang Chang Wen Ke Xilong Deng Yun Ling Shi Guan Wu Xue Feng Niu Ranawaka A Perera Yuan Da Xu Jincun Zhao Lin Qi Zhang Yi Min Li Rong Chang Chen Malik Peiris Ling Chen Nan Shan Zhong 《Emerging infectious diseases》2015,21(12):2278-2280
5.
D. J Keating H. Peiris M. Kyloh S. J. H. Brookes N. J. Spencer 《Neurogastroenterology and motility》2013,25(10):849-853
6.
YH Leung EH Lau LJ Zhang Y Guan BJ Cowling JS Peiris 《Emerging infectious diseases》2012,18(8):1339-1341
We analyzed ≈12 years of surveillance data on avian influenza in Hong Kong live poultry markets. A ban on keeping live poultry overnight in these markets reduced virus isolation rates by 84% in chickens (p = 0.006) and 100% (p = 0.01) in minor poultry. 相似文献
7.
8.
Comparison of P388D1 mouse macrophage cell line and human monocytes for assay of dengue-2 infection-enhancing antibodies 总被引:2,自引:0,他引:2
S B Halstead K Larsen S Kliks J S Peiris J Cardosa J S Porterfield 《The American journal of tropical medicine and hygiene》1983,32(1):157-163
Tissue culture-adapted dengue 2 virus (DEN 2), strain 16681, exhibits antibody-dependent enhancement of infection (ADE) in P388D1 cells, a mouse macrophage-like cell line. ADE is dependent upon maintaining DEN 2 multiplicity of infection at between 0.1 and 0.001, and can be simply measured in multi-well plastic plates. The assay uses either trypsinized or non-trypsinized P388D1 cells at 5 x 10(5) cells per ml, an appropriate dilution of DEN 2 virus, and a source of antibody, and is most conveniently performed without further washing of stationary cultures, which are incubated in 5% CO2. Trypsinization of P388D1 cells prior to the addition of virus-serum mixtures reduced infection in control cultures thus increasing ADE. When cells were washed after incubation of virus-serum mixtures for 1 hour, a paradoxical increase of infection in cultures exposed to virus plus normal serum was noted, which reduced the sensitivity of the ADE assay. Using human cord blood sera, ADE titers measured in human monocytes and P388D1 cells were closely similar. This convenient and economical assay will facilitate large scale biological and epidemiological studies of dengue virus enhancing antibodies. 相似文献
9.
10.
Seto WH Tsang D Yung RW Ching TY Ng TK Ho M Ho LM Peiris JS;Advisors of Expert SARS group of Hospital Authority 《Lancet》2003,361(9368):1519-1520
We did a case-control study in five Hong Kong hospitals, with 241 non-infected and 13 infected staff with documented exposures to 11 index patients with severe acute respiratory syndrome (SARS) during patient care. All participants were surveyed about use of mask, gloves, gowns, and hand-washing, as recommended under droplets and contact precautions when caring for index patients with SARS. 69 staff who reported use of all four measures were not infected, whereas all infected staff had omitted at least one measure (p=0.0224). Fewer staff who wore masks (p=0.0001), gowns (p=0.006), and washed their hands (p=0.047) became infected compared with those who didn't, but stepwise logistic regression was significant only for masks (p=0.011). Practice of droplets precaution and contact precaution is adequate in significantly reducing the risk of infection after exposures to patients with SARS. The protective role of the mask suggests that in hospitals, infection is transmitted by droplets. 相似文献