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1.
Objective To study the protective effects of adenovirus-mediated human beta-nerve growth factor gene (hNGFβ) transfer combined with iron fortified nutrition on blast hearing damage in guinea pigs. Methods Deafness was induced by blast (172dB SPL) in 35 healthy guinea pigs. Seven days after noise exposure, 10 guinea pigs were inoculated with adenovirus-mediated hNGFβ (Ad-hNGFβ) into the perilymphatic space (the gene group), another 10 guinea pigs were given hNGFβ combined with iron fortified nutritional diet (the combination group), still another 10 guinea pigs were inoculated with artificial perilymphatic fluid (APF), and 5 guinea pigs served as control, given neither medicine nor noise exposure. Auditory brainstem response (ABR) threshold shifts were monitored in the guinea pigs before blast exposure and after gene transfer. Changes in cochlear morphology were examined with immunohistochemical and HE staining. Results One week after successful inoculation of hNGFβ, it was noted that Ad-hNGFβ protein was expressed in each turn of the cochlea, and its intensity was almost equal. After gene transfer in the combination group and the gene group, ABR threshold shifts at week 1 and 4 were significantly smaller than those of the APF group. HE staining showed that the number of the spiral ganglion cells in the combination group and the Ad-hNGFβ group was significantly higher than that of the control group after 4 weeks (P<0.01). Conclusions Ad-hNGFβ combined with iron fortified nutritional diet was effective in the prevention and treatment of blast hearing damage of cochlear hair cells. 相似文献
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卫生类高职学校在教育资源的配置上发挥自身的能动作用,是市场机制调节的必然结果。在资源有限的情况下,既市场因素,又不能忽略高职教育培养人才的特殊性,把握其为社会服务的根本宗旨,处理好经济效益与社会效益的相互关系。 相似文献
4.
吴建 《国外医学:耳鼻咽喉科学分册》1992,(4)
内耳病毒感染可伴发耳聋。虽曾建立内耳病毒感染的动物模型,但病毒感染内耳的途径还不清楚。作者对18只雄豚鼠经右圆窗接种0.002ml Hanta病毒液入耳蜗,感染后4、6、8、10、12、16天各处死3只;2只雄豚鼠同样方法接种无病毒的培养液,作为对照;另18只雄豚鼠经腹腔内接种此病毒液1ml,其中6只于接种前3天给予环磷酰胺(CY,250mg/kg),6只接种前1天和接种后5天给予环孢霉素A 相似文献
5.
贯彻"三个代表"思想创建一流总医院 总被引:3,自引:1,他引:2
本文阐述了以“三个代表”思想为指导,从科技强院,文化兴院和质量建院三个方面加强全面建设的经验,为全面提高军队医院建设提供参考。 相似文献
6.
目的 对临床用药咨询进行分析,了解临床需要,提高临床药学服务质量。方法 收集整理400例临床药物咨询资料,对用药咨询的内容进行回顾性分析。结果 咨询中以用法用量最多(20.75%),同类药物比较居第二位(14.00%),并且归纳了门诊用药十大误区与结果。结论药物咨询可提高病人的用药依从性,确保其合理用药。 相似文献
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目的 研究腹泻型与便秘型肠易激综合征(IBS)病人直肠肛管动力和直肠感觉功能的不同特点。方法 选择IBS病人85例,分成两组。其中腹泻组52例,便秘组33例,20例健康志愿者为对照组,采用PC Polygraf ID高分辨率多道胃肠功能测定仪,分别测定直肠肛管压力、直肠感知阈值、排便阈值、最大耐受量和直肠肛门抑制反射最低充气量。结果IBS病人的直肠静息压、肛管静息压和直肠肛门抑制反射最低充气量与健康对照组比较无显著性差异。腹泻组的最大缩窄压和排便阈值显著低于健康对照组。便秘组的松弛压、直肠感知阈值、排便阈值和最大耐受量显著高于健康对照组。结论腹泻型IBS病人的症状与最大缩窄压和排便闽值降低有关;而便秘型则与松弛压、直肠感知阈值、排便阈值和最大耐受量显著增高有关。 相似文献
9.
采用双温套式PCR法检测血清HCVRNA,由通常的三温度循环改为二温度循环,节省时间,并可用2个恒温水浴锅聚代PCR扩增仪,结果与经典法测定无差异。 相似文献
10.
Objective:To study whether adenovirus-mediated human β-nerve growth factor (Ad-hNGFβ) gene has any protective effect on blast hearing impairment. Methods:Deafness was induced by blast exposure (172.0 dB) in 30 healthy guinea pigs. On day 7 of blast exposure, Ad-hNGFβ was infused into the perilymphatic space of 20 animals as the study group (hNGFβ group), and artificial perilymph fluid (APF) was infused into the perilymphatic space of the other 10 animals as the control group. At weeks 1, 4 and 8 after blast exposure, the animals were sacrificed and the cochleae were removed for immunohistochemical and HE stainings. Results: Expression of Ad-hNGFβ protein was detected in each turn of the cochlea at the 1st week, with almost equal intensity in all turns. At the 4th week, the reactive intensity of the expression of Ad-hNGFβ protein decreased. At the 8th week, no expression was detectable. The results of HE staining showed that the amount of spiral ganglions in hNGFβ group was significantly greater than that of the control group at week 4 (P〈0.01). Conclusion: Ad-hNGFβ can be expressed at a high level and for a relatively long period in the blast impaired cochlea, suggesting that Ad-hNGFβ has a protective effect on cochlear spiral ganglion cells after blast exposure and the efficient gene transfer into cochlea had been achieved without toxicity. 相似文献