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1.
Andrea Ferrari MD Daniel Orbach MD Michela Casanova MD Max M. van Noesel MD Pablo Berlanga MD Bernadette Brennan MD Nadege Corradini MD Reineke A. Schoot MD Gema L. Ramirez-Villar MD Lisa Lyngsie Hjalgrim MD Rita Alaggio MD Gabriela Guillen Burrieza MD Akmal Safwat MD Alison L. Cameron MD Rick R. van Rijn MD Veronique Minard-Colin MD Ilaria Zanetti BSc Gianni Bisogno MD Julia C. Chisholm MD Johannes H. M. Merks MD 《Cancer》2023,129(16):2542-2552
Background
Limited data exist on the clinical behavior of pediatric non-rhabdomyosarcoma soft tissue sarcomas (NRSTS) with distant metastases at onset, and a clear standard of care has not yet been defined.Methods
This cohort study reports on pediatric adult-type metastatic NRSTS enrolled in two concurrent prospective European studies, i.e., the randomized BERNIE study and the single-arm MTS 2008 study developed by the European paediatric Soft tissue sarcoma Study Group. Treatment programs were originally designed for patients with metastatic rhabdomyosarcoma, i.e., nine courses of multidrug chemotherapy (with or without bevacizumab in the BERNIE study), followed by 12 cycles of maintenance therapy, whereas radiotherapy and/or surgery (on primary tumor and/or metastases) were delayed until after seven courses of chemotherapy had been administered.Results
The study included 61 patients <21 years old treated from July 2008 to December 2016. The lung was the site of metastases in 75% of the cases. All patients received multi-agent chemotherapy, 44% had local therapy to primary tumor, and 18% had treatment of metastases. Median time to progression/relapse was 6 months. A high rate of tumor progression was observed during the initial part of the chemotherapy program. With a median follow-up of 41.5 months (range, 2–111 months), 3-year event-free survival and overall survival were 15.4% (95% confidence interval [CI], 7.6–25.7) and 34.9% (95% CI, 22.7–47.5), respectively. There were no statistically significant differences in outcome depending on the type of treatment administered.Conclusions
The study confirmed the overall poor outcome for patients with metastatic NRSTS, whose treatment remains a challenge.Plain Language Summary
- Pediatric non-rhabdomyosarcoma soft tissue sarcomas form a heterogeneous group of rare tumors.
- Although recent international studies have defined the standard of care for patients with localized disease, limited data are available on the clinical behavior of patients with distant metastases.
- This study on 61 metastatic cases treated on two prospective European protocols confirms that the chances of survival of such patients are often dismal and a standard treatment is still lacking.
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Recellularizing of human acellular dermal matrices imaged by high‐definition optical coherence tomography 下载免费PDF全文
Marc A. L. M. Boone Jean Pierre Draye Gunther Verween Annalisa Aiti Jean‐Paul Pirnay Gilbert Verbeken Daniel De Vos Thomas Rose Serge Jennes Gregor B. E. Jemec Veronique del Marmol 《Experimental dermatology》2015,24(5):349-354
High‐definition optical coherence tomography (HD‐OCT) permits real‐time 3D imaging of the impact of selected agents on human skin allografts. The real‐time 3D HD‐OCT assessment of (i) the impact on morphological and cellular characteristics of the processing of human acellular dermal matrices (HADMs) and (ii) repopulation of HADMs in vitro by human fibroblasts and remodelling of the extracellular matrix by these cells. Four different skin decellularization methods, Dispase II/Triton X‐100, Dispase II/SDS (sodium dodecyl sulphate), NaCl/Triton X‐100 and NaCl/SDS, were analysed by HD‐OCT. HD‐OCT features of epidermal removal, dermo‐epidermal junction (DEJ) integrity, cellularity and dermal architecture were correlated with reflectance confocal microscopy (RCM), histopathology and immunohistochemistry. Human adult dermal fibroblasts were in vitro seeded on the NaCl/Triton X‐100 processed HADMs, cultured up to 19 days and evaluated by HD‐OCT in comparison with MTT proliferation test and histology. Epidermis was effectively removed by all treatments. DEJ was best preserved after NaCl/Triton X‐100 treatment. Dispase II/SDS treatment seemed to remove all cellular debris in comparison with NaCl/Triton X‐100 but disturbed the DEJ severely. The dermal micro‐architectural structure and vascular spaces of (sub)papillary dermis were best preserved with the NaCl/Triton X‐100. The impact on the 3D structure and vascular holes was detrimental with Dispase II/SDS. Elastic fibre fragmentation was only observed after Dispase II incubation. HD‐OCT showed that NaCl/Triton X‐100 processed matrices permitted in vitro repopulation by human dermal fibroblasts (confirmed by MTT test and histology) and underwent remodelling upon increasing incubation time. Care must be taken in choosing the appropriate processing steps to maintain selected properties of the extracellular matrix in HADMs. Processing HADMs with NaCl/Triton X‐100 permits in vitro the proliferation and remodelling activity of human dermal fibroblasts. HD‐OCT provides unique real‐time and non‐invasive 3D imaging of tissue‐engineered skin constructs and complementary morphological and cytological information. 相似文献
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Hendrickx Gaelle De Roeck Veronique Russet Frdrick Dieleman Gwen Franic Tomislav Maras Athanasios McNicholas Fiona Paul Moli Santosh Paramala Schulze Ulrike Signorini Giulia Singh Swaran P. Street Cathy Tuomainen Helena Verhulst Frank Wolke Dieter Purper-Ouakil Diane Tremmery Sabine 《European child & adolescent psychiatry》2020,29(1):41-49
European Child & Adolescent Psychiatry - The majority of adolescents with mental health problems do not experience continuity of care when they reach the transition boundary of their child and... 相似文献
6.
Liu Shuling Matthew Lau Sie Kuei Seyed Ehsan Saffari Zheng Jiayun Tan Tse Yeun Jessie Phoon Wai Leng Veronique Viardot-Foucault Sadhana Nadarajah Jerry Kok Yen Chan Tan Heng Hao 《Reproductive biomedicine online》2019,38(1):39-45
Research question
Previous studies of aromatase inhibitors on male infertility have focused on men with low testosterone–oestradiol ratio of less than 10. Can aromatase inhibitors improve spermatogenesis in men with idiopathic male infertility with normal testosterone–oestradiol ratio?Design
Prospective study of men with idiopathic severe oligozoospermia (sperm concentration <5 million/ml) carried out between February 2015 and March 2017. The objective was to assess if semen-analysis parameters improved after treatment with letrozole. Secondary objectives were to monitor the safety of letrozole in men, and to measure the alterations in serum FSH, LH, oestradiol and testosterone levels.Results
Fifteen men with normal testosterone–oestradiol ratio (>10) were treated with letrozole 2.5 mg daily for 4 months. This produced a 5.5-fold increase in sperm concentration (P?=?0.0068). All men had increased total serum testosterone and suppressed oestradiol levels after treatment, thus raising the overall testosterone–oestradiol ratio (P < 0.0001). Adverse effects from letrozole were relatively minor and included loss of libido (54%), headaches (25%), fatigue (21%), weakness (13%), loss of hair (8%) and dry mouth (8%).Conclusions
Letrozole improves sperm concentration and increases testosterone–oestradiol ratio for men with oligozoospermia who have normal testosterone–oestradiol ratio; its role in the treatment of male infertility may be extended to this group of patients. In addition, it is a relatively well-tolerated drug with no serious adverse effects. 相似文献7.
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Baboudjian Michael Bandelier Quentin Gondran-Tellier Bastien Abdallah Rony Michel Floriane Sichez Pierre Clement Di-Crocco Eugenie Akiki Akram Gaillet Sarah Delaporte Veronique Andre Marc Daniel Laurent Karsenty Gilles Lechevallier Eric Boissier Romain 《International urology and nephrology》2020,52(4):611-618
International Urology and Nephrology - In our center, until 2018, MRI-targeted biopsy was underused. Since January 2018, we systematically performed MRI-targeted biopsy for suspicious... 相似文献
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Rita Deroisy Julian Collette Thierry Chevallier Veronique Breuil Jean Yves Reginster 《Current therapeutic research》1998,59(12):850-862
The aim of this study was to compare the effects of a 1-year treatment with calcium and vitamin D3 combined in a tablet (Orocal®) with the effects of a reference treatment of calcium administered in a sachet (Ostram®) and vitamin D3 as a tablet (Devaron®) on bone remodeling markers and bone mineral density (BMD) in elderly women. The study population comprised 119 women ?70 years of age, 80% of whom were institutionalized. The women were randomly allocated to receive treatment for 1 year with elemental calcium 0.5 g and vitamin D3 400 IU combined in one chewable tablet taken twice daily (group 1) or elemental calcium 0.6 g (1 sachet) and vitamin D3 400 IU (1 chewable tablet) taken twice daily (group 2). BMD of the proximal femur was measured at four sites before and after the 1-year treatment period; serum 1–84 intact parathyroid hormone (PTH) concentration, serum 25-hydroxyvitamin D3 (25[OH]D3) concentration, and bone alkaline phosphatase activity were measured at baseline and after 6 and 12 months of treatment. At month 12, BMD had increased at all sites in patients in group 1; the increase was statistically significant at the trochanter (2.34%). In group 2, BMD had increased slightly at the femoral neck and decreased slightly at the other sites, without any statistically significant differences. BMD changes were not statistically different between the two groups except at the trochanter, where we observed a 2.34% increase in group 1 versus a 0.32% decrease in group 2. In group 1, PTH decreased significantly from baseline to month 6 (13%) and then did not change significantly from month 6 to month 12. In group 2 the decrease was not statistically significant. In addition, 25(OH)D3 increased significantly in the two groups from baseline to month 6 (group 1, 107%; group 2, 96%). This significant increase continued but became less marked from month 6 to month 12 (13% and 15%, respectively). Bone alkaline phosphatase values decreased significantly in both groups from baseline to month 6; however, this decrease was significantly greater in group 1 (35%) than in group 2 (22%). From month 6 to month 12, values remained constant in both groups. In this study, results with the calcium and vitamin D3 combination tablet were comparable to those of the separated calcium and vitamin D3 supplementation concerning parameters involved in senile osteoporosis. Densitometric data showed some prevention of age-related bone loss; biochemical data showed the correction of vitamin D3 deficiency and the reduction of PTH secretion, both of which are responsible for increased bone remodeling in the elderly. The results of this study confirm those of previous studies on the effects of calcium and vitamin D3 supplementation in institutionalized elderly patients. 相似文献
10.
Marolleau JP Vanneaux V Rea D Ternaux B Delasse V Hubert V Chantre E Traineau R Robin M Benbunan M Socié G Larghero J 《Transfusion》2007,47(2):266-271
BACKGROUND: In allogeneic bone marrow transplantation (BMT), a higher nucleated and CD34+ cell dose has been reported to improve various outcomes. Other cell types, such as lymphocyte subsets, also influenced BMT results. While nucleated red blood cells (NRBCs) represent a subset of bone marrow (BM) cell subpopulation, the question of their quantification in BM grafts and the impact of BM processing on their recovery has not been addressed. STUDY DESIGN AND METHODS: In a prospective study on 77 BM products, NRBCs were enumerated by flow cytometry and the recovery analyzed after manipulation. Because NRBCs could compromise white blood cell count, the impact of NRBC count on CD34+ cell percentage and total nucleated cell (TNC) dose were also determined. RESULTS: The mean percentage of NRBCs in BM grafts was 21.6 percent (range, 7.8%-40.9%). Mean NRBC recoveries after BM concentration or RBC depletion were 98.4 and 28.7 percent, respectively, close to those obtained for TNC cells (88.6 and 31.3%, respectively). When corrected with NRBC count, the mean percentages of corrected CD34+ cell and TNC dose were significantly modified when compared with uncorrected values, whatever the type of BM manipulation. CONCLUSION: Our data show that NRBC quantification might be of importance to improve quality control of BM products and to evaluate the influence of NRBCs cell dose on outcomes after BMT. 相似文献