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排序方式: 共有1916条查询结果,搜索用时 15 毫秒
1.
Elena Andreucci Anna Laurenzana Silvia Peppicelli Alessio Biagioni Francesca Margheri Jessica Ruzzolini Francesca Bianchini Gabriella Fibbi Mario Del Rosso Chiara Nediani Simona Serratì Livia Fucci Michele Guida Lido Calorini 《Oncology research》2020,28(9):873-884
Malignant melanoma is a highly aggressive skin cancer characterized by an elevated grade of tumor cell plasticity. Such plasticity allows adaptation of melanoma cells to different hostile conditions and guarantees tumor
survival and disease progression, including aggressive features such as drug resistance. Indeed, almost 50% of
melanoma rapidly develop resistance to the BRAFV600E inhibitor vemurafenib, with fast tumor dissemination,
a devastating consequence for patients’ outcomes. Vasculogenic mimicry (VM), the ability of cancer cells to
organize themselves in perfused vascular-like channels, might sustain tumor spread by providing vemurafenibresistant cancer cells with supplementary ways to enter into circulation and disseminate. Thus, this research
aims to determine if vemurafenib resistance goes with the acquisition of VM ability by aggressive melanoma
cells, and identify a driving molecule for both vemurafenib resistance and VM. We used two independent
experimental models of drug-resistant melanoma cells, the first one represented by a chronic adaptation of
melanoma cells to extracellular acidosis, known to drive a particularly aggressive and vemurafenib-resistant
phenotype, the second one generated with chronic vemurafenib exposure. By performing in vitro tube formation assay and evaluating the expression levels of the VM markers EphA2 and VE-cadherin by Western blotting
and flow cytometer analyses, we demonstrated that vemurafenib-resistant cells obtained by both models are
characterized by an increased ability to perform VM. Moreover, by exploiting the CRISPR-Cas9 technique and
using the urokinase plasminogen activator receptor (uPAR) inhibitor M25, we identified uPAR as a driver of
VM expressed by vemurafenib-resistant melanoma cells. Thus, uPAR targeting may be successfully leveraged
as a new complementary therapy to inhibit VM in drug-resistant melanoma patients, to counteract the rapid
progression and dissemination of the disease. 相似文献
2.
Xiaoxuan Liu Ameenat L. Solebo Livia Faes Sophie Beese Tasanee Braithwaite Matthew E. Round 《Ocular immunology and inflammation》2020,28(6):898-907
ABSTRACT
Purpose
New instrument-based techniques for anterior chamber (AC) cell counting can offer automation and objectivity above clinician assessment. This review aims to identify such instruments and its correlation with clinician estimates. 相似文献3.
4.
Debora Lopes Salles Scheffel Jaime Aparecido Cury Livia Maria Andal Tenuta Rgis Henke Scheffel Cristina Perez Diana Gabriela Soares Fernanda Gonalves Basso Carlos Alberto de Souza Costa David Henry Pashley Josimeri Hebling 《International journal of paediatric dentistry / the British Paedodontic Society [and] the International Association of Dentistry for Children》2020,30(5):650-659
5.
6.
Simona Petrucci MD PhD Monia Ginevrino PhD Ilaria Trezzi MD Edoardo Monfrini MD Lucia Ricciardi MD PhD Alberto Albanese MD Micol Avenali MD Paolo Barone MD Anna Rita Bentivoglio MD PhD Vincenzo Bonifati MD PhD Francesco Bove MD Laura Bonanni MD PhD Livia Brusa MD Cristina Cereda PhD Giovanni Cossu MD Chiara Criscuolo MD Giovanna Dati BSc Anna De Rosa MD PhD Roberto Eleopra MD Giovanni Fabbrini MD Laura Fadda MD PhD Manuela Garbellini BSc Brigida Minafra MD Marco Onofrj MD Claudio Pacchetti MD Ilaria Palmieri BSc Maria Teresa Pellecchia MD PhD Martina Petracca MD Marina Picillo MD PhD Antonio Pisani MD PhD Annamaria Vallelunga PhD Roberta Zangaglia MD Alessio Di Fonzo MD PhD Francesca Morgante MD PhD Enza Maria Valente MD PhD ITA-GENE-PD Study Group 《Movement disorders》2020,35(11):2106-2111
7.
Giovanne B. Diniz Daniella S. Battagello Marianne O. Klein Bianca S. M. Bono Jozélia G. P. Ferreira Livia C. Motta-Teixeira Jessica C. G. Duarte Françoise Presse Jean-Louis Nahon Antoine Adamantidis Melissa J. Chee Luciane V. Sita Jackson C. Bittencourt 《Journal of neuroscience research》2020,98(10):2045-2071
Melanin-concentrating hormone (MCH) is a ubiquitous vertebrate neuropeptide predominantly synthesized by neurons of the diencephalon that can act through two G protein-coupled receptors, called MCHR1 and MCHR2. The expression of Mchr1 has been investigated in both rats and mice, but its synthesis remains poorly described. After identifying an antibody that detects MCHR1 with high specificity, we employed immunohistochemistry to map the distribution of MCHR1 in the CNS of rats and mice. Multiple neurochemical markers were also employed to characterize some of the neuronal populations that synthesize MCHR1. Our results show that MCHR1 is abundantly found in a subcellular structure called the primary cilium, which has been associated, among other functions, with the detection of free neurochemical messengers present in the extracellular space. Ciliary MCHR1 was found in a wide range of areas, including the olfactory bulb, cortical mantle, striatum, hippocampal formation, amygdala, midline thalamic nuclei, periventricular hypothalamic nuclei, midbrain areas, and in the spinal cord. No differences were observed between male and female mice, and interspecies differences were found in the caudate-putamen nucleus and the subgranular zone. Ciliary MCHR1 was found in close association with several neurochemical markers, including tyrosine hydroxylase, calretinin, kisspeptin, estrogen receptor, oxytocin, vasopressin, and corticotropin-releasing factor. Given the role of neuronal primary cilia in sensing free neurochemical messengers in the extracellular fluid, the widespread distribution of ciliary MCHR1, and the diverse neurochemical populations who synthesize MCHR1, our data indicate that nonsynaptic communication plays a prominent role in the normal function of the MCH system. 相似文献
8.
9.
Cristina Fiorani Livia Biancone Giorgia Tema Kristina Porokhnavets Manfredi Tesauro Achille L. Gaspari Giuseppe S. Sica 《JSLS, Journal of the Society of Laparoendoscopic Surgeons》2014,18(3)
Midgut malrotation is an anomaly of fetal intestinal rotation. Its incidence in adults is rare. A case of midgut malrotation in a 51-year-old man with complicated Crohn''s disease of the terminal ileum is presented. Symptoms, diagnosis, and treatment are reviewed. Preoperative workup led to correct surgical planning that ultimately allowed a successful laparoscopic resection. 相似文献