全文获取类型
收费全文 | 270篇 |
免费 | 40篇 |
国内免费 | 4篇 |
专业分类
妇产科学 | 1篇 |
基础医学 | 95篇 |
口腔科学 | 1篇 |
临床医学 | 17篇 |
内科学 | 138篇 |
皮肤病学 | 2篇 |
神经病学 | 1篇 |
特种医学 | 3篇 |
外科学 | 7篇 |
综合类 | 3篇 |
预防医学 | 33篇 |
药学 | 2篇 |
肿瘤学 | 11篇 |
出版年
2023年 | 5篇 |
2022年 | 8篇 |
2021年 | 10篇 |
2020年 | 8篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 11篇 |
2015年 | 11篇 |
2014年 | 14篇 |
2013年 | 10篇 |
2012年 | 17篇 |
2011年 | 29篇 |
2010年 | 20篇 |
2009年 | 14篇 |
2008年 | 19篇 |
2007年 | 20篇 |
2006年 | 7篇 |
2005年 | 13篇 |
2004年 | 8篇 |
2003年 | 9篇 |
2002年 | 7篇 |
2001年 | 2篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 2篇 |
排序方式: 共有314条查询结果,搜索用时 15 毫秒
1.
Epitope mapping of the influenza A virus RNA polymerase PA using monoclonal antibodies 总被引:1,自引:0,他引:1
Hatta M Asano Y Masunaga K Ito T Okazaki K Toyoda T Kawaoka Y Ishihama A Kida H 《Archives of virology》2000,145(5):957-964
Summary. To obtain reagents to functionally map the PA protein, we produced monoclonal antibodies specific to this protein. Twenty-two
monoclonal antibodies reacting with PA protein in ELISA were divided into 10 groups on the basis of competitive binding patterns
to this protein. Of these, seventeen monoclonal antibodies bound to PA polypeptide spanning amino acids 101–400 and three
bound to that of amino acids 518–600, while the other two did not react with any PA polypeptides tested with the exception
of full-length PA. Among these monoclonal antibodies, only five reacted with PA in A/PR/8/34 virus-infected cells in indirect
immunofluorescence assay. Thus, we obtained monoclonal antibodies that recognize at least 10 distinct regions of the PA molecule.
These monoclonal antibodies should be useful in dissecting functions of the PA protein.
Received September 6, 1999/Accepted January 5, 2000 相似文献
2.
3.
Takayuki Fujii Masao Hagihara Keiko Mitamura Shiori Nakashima Shin Ohara Tomoyuki Uchida Morihiro Inoue Moe Okuda Atsuhiro Yasuhara Jurika Murakami Calvin Duong Kiyoko Iwatsuki-Horimoto Seiya Yamayoshi Yoshihiro Kawaoka 《Internal medicine (Tokyo, Japan)》2022,61(11):1681
Objective Coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally. Although the relationship between anti-SARS-CoV-2 immunoglobulin G (IgG) antibodies and COVID-19 severity has been reported, information is lacking regarding the seropositivity of patients with particular types of diseases, including hematological diseases. Methods In this single-center, retrospective study, we compared SARS-CoV-2 IgG positivity between patients with hematological diseases and those with non-hematological diseases. Results In total, 77 adult COVID-19 patients were enrolled. Of these, 30 had hematological disorders, and 47 had non-hematological disorders. The IgG antibody against the receptor-binding domain of the spike protein was detected less frequently in patients with hematological diseases (60.0%) than in those with non-hematological diseases (91.5%; p=0.029). Rituximab use was significantly associated with seronegativity (p=0.010). Conclusion Patients with hematological diseases are less likely to develop anti-SARS-CoV-2 antibodies than those with non-hematological diseases, which may explain the poor outcomes of COVID-19 patients in this high-risk group. 相似文献
4.
Daisuke Miyaki Hiroshi Aikata Yohji Honda Noriaki Naeshiro Takashi Nakahara Mio Tanaka Yuko Nagaoki Tomokazu Kawaoka Shintaro Takaki Koji Waki Akira Hiramatsu Shoichi Takahashi Masaki Ishikawa Hideaki Kakizawa Kazuo Awai Kazuaki Chayama 《Journal of gastroenterology and hepatology》2012,27(12):1850-1857
5.
Daisuke Miyaki Hiroshi Aikata Hiromi Kan Hatsue Fujino Ayako Urabe Keiichi Masaki Takayuki Fukuhara Tomoki Kobayashi Noriaki Naeshiro Takashi Nakahara Tomokazu Kawaoka Akira Hiramatsu Shoichi Takahashi Masaki Ishikawa Hideaki Kakizawa Kazuo Awai Kazuaki Chayama 《Journal of gastroenterology and hepatology》2013,28(12):1834-1841
6.
Tomio Ueno Koutaro Yamamoto Toru Kawaoka Motonari Takashima Masaaki Oka 《Journal of hepato-biliary-pancreatic sciences》2005,12(4):304-309
Background/Purpose
The aim of this study was to investigate the current use of antibiotic prophylaxis (AP) in association with pancreatoduodenectomy (PD) in Japan, and to determine its surgical implications.Methods
We surveyed 2331 patients who underwent PD for treatment of disease in the periampullary region. Data, obtained during the period January 2002 through December 2003, from 111 major surgical services associated with the Japanese Society for Pancreatic Surgery, were analyzed with regard to patient characteristics, preoperative complications, AP, and postoperative morbidities.Results
Eighty-five (78.7%) of the 108 eligible institutions chose a first- or second-generation cephalosporin for AP, given for a mean duration of 4.3 days. At all but 1 institution, the first dose was administered prior to surgical incision of the skin. At 42% of the institutions, an additional antibiotic was administered during surgery. The overall rate of wound infection was 6.8% of the 2266 patients for whom data were available. Preoperative jaundice was found in 55.3% of these 2266 patients, and 92.6% of these jaundiced patients were suffering from preoperative infections. In addition, those with preoperative infections were also diagnosed as having biliary infections. The number of patients with preoperative jaundice in combination with preoperative infections was significantly related to the rate of postoperative morbidity (P < 0.0001).Conclusions
Administration of AP in association with PD in Japan seems appropriate. Icteric patients with biliary infections are at high risk for postoperative morbidities and need careful monitoring after surgery. 相似文献7.
Sequence requirements for cleavage activation of influenza virus hemagglutinin expressed in mammalian cells. 总被引:33,自引:1,他引:33 下载免费PDF全文
Y Kawaoka R G Webster 《Proceedings of the National Academy of Sciences of the United States of America》1988,85(2):324-328
Cleavage of the hemagglutinin (HA) in tissue culture systems has been correlated with virulence of avian influenza viruses. To examine the structural requirements for cleavage of the HA, the HA gene from a virulent H5 influenza virus was expressed in mammalian cells (CV-1), and the cleavage site of the HA was explored by using site-specific mutagenesis. The expressed HA protein exhibited normal cleavage, transport to the cell membrane, and ability to adsorb and to fuse erythrocytes at pH 5. Site-specific mutagenesis of the HA directly established that (i) most of the basic amino acids at this site are critical for cleavage activation; (ii) besides the connecting peptide sequence, at least one other structural feature of the HA is required for enzyme recognition; and (iii) the length of the connecting peptide can abrogate the structural feature(s). 相似文献
8.
M. Imai S. Herfst E.M. Sorrell E.J.A. Schrauwen M. Linster M. De Graaf R.A.M. Fouchier Y. Kawaoka 《Virus research》2013
Highly pathogenic avian H5N1 influenza A viruses occasionally infect humans and cause severe respiratory disease and fatalities. Currently, these viruses are not efficiently transmitted from person to person, although limited human-to-human transmission may have occurred. Nevertheless, further adaptation of avian H5N1 influenza A viruses to humans and/or reassortment with human influenza A viruses may result in aerosol transmissible viruses with pandemic potential. Although the full range of factors that modulate the transmission and replication of influenza A viruses in humans are not yet known, we are beginning to understand some of the molecular changes that may allow H5N1 influenza A viruses to transmit via aerosols or respiratory droplets among mammals. A better understanding of the biological basis and genetic determinants that confer transmissibility to H5N1 influenza A viruses in mammals is important to enhance our pandemic preparedness. 相似文献
9.
Noriaki Naeshiro Hideaki Kakizawa Hiroshi Aikata Hiromi Kan Hatsue Fujino Takayuki Fukuhara Tomoki Kobayashi Yohji Honda Daisuke Miyaki Tomokazu Kawaoka Masataka Tsuge Akira Hiramatsu Michio Imamura Yoshiiku Kawakami Hideyuki Hyogo Masaki Ishikawa Kazuo Awai Kazuaki Chayama 《Hepatology research》2014,44(7):740-749