高脂模型大鼠代谢特征分析

目的:观察高脂饲料喂养对SD大鼠糖脂代谢的影响.方法:将20只雄性SD大鼠随机分成普食组(Normal)和高脂组(HFD),每周监测体质量,喂养8周后采用OGTT和ITT检测胰岛素抵抗,自动分析仪检测血糖血脂,称量肝脏、肾周和附睾脂肪重量,免疫组化分析组织形态.结果:与普食组相比,高脂大鼠体质量及肾周、附睾脂肪重量明显...     

上调 Notch－1基因对 U215细胞 AKT －mTOR 通路的影响

目的：观察上调 Notch －1基因对人胶质瘤 U251细胞生学物行为及 AKT －mTOR 信号通路的影响。方法：采用 pNL －NICD 慢病毒感染 U251细胞,以 RT －PCR 和 Western －Blot 检测 Notch －1基因 mRNA 和蛋白的表达,以 MTT 检测细胞增殖能力,流式细胞术检测细胞周期变化,Transwell 实验检测细胞侵袭能力,同时检测 Notch －1上调对 AKT、mTOR、P70S6K、4Ebp －1蛋白的影响。结果：与对照组比较,NICD 慢病毒转染72h后,Notch －1基因 mRNA 和蛋白表达明显增高（P ＜0．01）;Notch －1上调明显促进 U251细胞增殖、侵袭、促进细胞进入 S 期,增加周期蛋白 Cyclin D1、CDK －4的表达,促进 AKT、mTOR 蛋白磷酸化。结论：上调 Notch －1基因促进 U251细胞增殖、侵袭,其机制和活化 AKT －mTOR 通路有关。     

平糖方对痰瘀证代谢综合征的临床疗效观察

目的：观察平糖方对代谢综合征患者中医病理证素积分和代谢指标的影响。方法：选择70例痰瘀证代谢综合征患者,随机分为平糖方组和安慰剂。干预8周,采用证素积分评价中医病位、病性证素变化,并比较治疗前后血糖、血脂等代谢指标的变化。结果：平糖方治疗后,病位证素脾和病性证素痰、湿、瘀的积分均较治疗前明显降低（P＜0.05）。平糖方组患者的体重较治疗前减少;血清胆固醇和甘油三酯、120min血糖和胰岛素水平较治疗前降低（P＜0.05）。结论：平糖方能降低代谢综合征患者的脾、痰、湿、瘀病理证素积分,改善糖脂代谢紊乱,减轻胰岛素抵抗。     

糖尿病患者生存质量研究的近况及展望

文章综述了糖尿病生存质量的研究现状,包括生存质量的涵义、生存质量评价的必要性、生存质量量表、影响因素、干预措施及目前存在的问题,并对今后的研究方向做出展望。     

血脂康胶囊治疗高尿酸血症疗效和安全性的随机双盲临床试验

目的评价血脂康胶囊治疗高尿酸血症的疗效和安全性。方法采用随机、双盲、安慰剂、对照的设计,将80例高尿酸血症患者随机分为两组,试验组和对照组各40例,进行治疗和比较,疗程为28d。结果共有75例完成了试验(试验组38例,对照组37例)。与用药前相比,试验组的尿酸、黄嘌呤氧化酶、空腹血浆胰岛素、胰岛素抵抗指数、三酰甘油、胆固醇、脂蛋白、超敏C反应蛋白、内皮素、尿β2微球蛋白下降和高密度脂蛋白升高均有统计学意义。试验过程中两组均未出现不良反应。结论血脂康胶囊治疗高尿酸血症具有很好的疗效和安全性。     

《中国2型糖尿病防治指南(2020年版)》推荐中成药品种述评

中华医学会糖尿病学分会发布《中国2型糖尿病防治指南(2020年版)》(以下简称"2020年版《指南》")阐述了中医药在防治糖尿病中的作用.中成药是中医防治糖尿病的重要手段,本文结合2020年版《指南》推荐的中成药品种,分析其组成、功效及循证证据等,就中成药在临床应用中的定位及研究方向进行探讨,为充分发挥中成药在2型糖尿...     

根据疏泄理论从古中医经方角度解读桂枝汤

桂枝汤为《伤寒杂病论》第一方,此处“第一”的意义包含第一个出现的方剂和加减运用最广泛的方剂双重含义。如柯琴在《伤寒来苏集》中所说:“此为仲景群方之魁,乃滋阴和阳,解肌发汗,调和营卫之主方……不拘何经,不论中风、杂病,咸得用此”。现本文根据杨叔禹教授的疏泄理论,对桂枝汤进行解读,兹介绍如下。     

Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats

Objective:To investigate the therapeutic effects of Ping-tang Recipe(平糖方,PTR) on highfat diet(HFD)-induced insulin resistance and non-alcoholic fatty liver disease(NAFLD),and to elucidate the underlying mechanisms.Methods:Forty male SD rats were included in the study.Ten rats were fed on normal diet as normal control,and thirty rats were fed on HFD for 8 weeks to induce obesity,followed with low dose (0.42 g/kg) or high dose(0.84 g/kg) of PTR or vehicle for 8 weeks with 10 animals for each group.Glucose metabolism and insulin sensitivity were evaluated by oral glucose tolerance test and insulin tolerance test. Hepatic steatosis was measured by immunohistochemistry.Liver lipid metabolic genes were analyzed by quantitative real-time polymerase chain reaction,while AMP-activated protein kinase(AMPK) expression was examined by Western blot.Results:Rats fed on HFD developed abdominal obesity,insulin resistance and NAFLD.PTR treatment reduced visceral fat(peri-epididymal and peri-renal) accumulation,improved glucose metabolism,and attenuated hepatic steatosis.The expressions of the key lipolytic regulating genes,including peroxisome proliferators-activated receptorγco-activator 1α(PGC-1α),peroxisome proliferator-activated receptorγ(PRAR-γ) andα(PRAR-α),were up-regulated(P<0.05 or P<0.01),while the expressions of lipogenic genes such as sterol regulatory element-binding protein 1c(SREBP-1c),fatty acid synthase (FAS) and liver fatty acid-binding protein(L-FABP) were down-regulated(P<0.05 or P<0.01).In addition, PTR activated AMPK and promoted acetyl-CoA carboxylase phosphorylation in the liver.Conclusions:PTR improves insulin resistance and reverse hepatic steatosis in the rat model of HFD-induced obesity through promotion of lipolysis and reduction of lipogenesis,which involves the AMPK signaling pathway,thus representing a new therapeutic intervention for obesity related insulin resistance and NAFLD.