nm23-H1基因转染对人胆管癌细胞系QBC939体外浸润能力的影响

目的：探讨nm23-H1基因转染对人胆管癌细胞系QBC939体外浸润能力的影响。方法：将含有全长nm23-H1 cDNA的真核表达载体通过脂腩体法转染人胆管癌细胞系。结果：转染成功的QBC939细胞，其nm23-Hl基因的mRNA、蛋白表达明显增加，转染nm23-H1基因的胆管癌细胞体外浸润能力下降，穿越matrigel的细胞数明显低于亲本QBC939细胞，代表浸润能力的IV型胶原酶（MMP-9）分泌量下降。结论：nm23-Hl基因可以抑制胆管癌细胞的体外浸润能力。     

腹腔镜胆囊切除术前对合并症的诊治及术中对胆道损伤的预防

腹腔镜胆囊切除术前对合并症的诊治及术中对胆道损伤的预防周永碧龚建平王曙光腹腔镜胆囊切除术（ｌａｐａｒｏｓｃｏｐｉｃｃｈｏｌｅｃｙｓｔｅｃ－ｔｏｍｙ，简称ＬＣ）已在全世界得到推广，但如果术前对胆囊疾病患者的合并疾病认识不足，术中处理不当，术后会出现多种...     

改良犬原位背驮式肝移植的解剖学观察

目的观察犬肝脏解剖学特点,探索建立改良的犬同种异体原位背驮式肝移植模型。方法对15只杂交犬的肝脏进行活体和尸体标本的解剖观察。结果犬肝共分7叶,门静脉分左右支进入肝脏,门静脉左支供应约70%肝组织(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ叶),门静脉右支供应约30%肝组织(Ⅵ、Ⅶ叶),分别阻断门静脉左、右支后5 min左右门静脉压力达到峰值,并迅速回复至门静脉正常压力值上限范围内;肝动脉和胆管与门静脉分支伴行进入各肝叶;左肝静脉粗大,可以作为肝静脉重建的流出道,其余3~6支肝静脉可以分别结扎,肝后下腔静脉易于与肝脏分离。结论犬肝的解剖学特点适宜于建立改良的原位背驮式肝移植模型。     

Effects of α1-adrenergic receptor on the proliferation of cholangiocarcinoma cells

Objective To investigate the correlation between α1-adrenergic receptor and the pathological behavior of cholangiocarcinoma, and the effects of norepinephrine (NE) on the proliferation of cholangiocarcinoma cell line QBC939. Methods Thirty-six samples of cholangiocarcinoma were resected in Southwest Hospital from August 2002 to March 2008. The expression of α1-adrenergic receptor in the 36 samples of cholangiocarcinoma tissue and 4 samples of normal bile duct tissue were detected by SABC technique. The proliferation of cholangio-carcinoma cell line QBC939 was detected after processing the cells with NE, phentolamine and prazosin. All the data were analyzed by chi-square test. Results The high positive expression rate of α1-adrenergic receptor was 68% (17/25) in patients with lymph node metastasis, which was significantly higher than 9% (1/11) in patients without lymph node metastasis (χ2=10.604, P<0.05). The high positive expression rate of α1-adrenergic receptor was 85% (11/13) in patients with middle and low positioned cholangiocarcinoma, which was significantly higher than 30% (7/23) in patients with hilar cholangiocarcinoma (χ2=9.753, P<0.05). NE promoted the proliferation of cholangiocarcinoma cell line QBC939 by stimulating the expression of α1-adrenergic receptor, and in a concentration-dependent manner. The proliferative effect was weakened as time passed by, and it was eliminated by phentolamine and prazosin. Conclusions The expression of α1-adrenergic receptor is diverse due to lymph node metastasis and the location of the tumor, α1-adrenergic receptor with high expression may play an important role in the proliferation and metastasis of cholangiocarcinoma.     

肝移植术后胆道并发症的发生原因与对策

肝移植术后胆道并发症发生率高达10％～34％，严重影响肝移植受者生存时间及生活质量，是肝移植后居第2位的主要并发症。随着对该并发症的进一步认识和外科技术的提高，外科技术原因造成的吻合口、引流管相关并发症发生率呈下降趋势，而非外科技术原因造成的胆道并发症变则成了主要类型。因此，被称为“阿基里斯之踵再现”。     

胆囊收缩素、胃泌素对胆管癌细胞细胞内钙的调节

目的:探索胆囊收缩素(CCK)、胃泌素对胆管癌细胞细胞内钙的调控作用。方法:应用细胞内钙测定技术,检测了CCK、胃泌素及其受体拮抗剂对胆管癌细胞QBC939(QB)细胞内钙的影响。结果:CCK-8S、G-17明显促进了胆管癌细胞QB细胞     

年龄因素对健康人心率变异性的影响

目的探讨年龄因素对健康人心率变异性（ＨＲＶ）的影响。材料与方法将２３６例健康人分为五个年龄组，使用美国先进医用设备公司５．０版本ＨＲＶ软件做短时时域及频域ＨＲＶ分析。结果年龄因素对健康人ＨＲＶ短时时域和频域分析的多项指标产生显著影响，随着年龄增大，时域指标中的ＨＲＳＤ、ＳＤＡＮＮ、ｒＭＳＳＤ及ＰＮＮ５０均逐渐降低；随着年龄增长，频域指标中各频段下的绝对面积，即：ＶＬＦ、ＬＦ、ＭＦ及ＨＦ均逐渐降低；而频域指标中各频段下的相对面积，即：ＶＬＦＰ、ＬＦＰ、ＭＦＰ、ＨＦＰ随着年龄增长呈不同的发展趋势。ＬＦ／ＨＦ随着年龄的增加而逐渐升高。结论ＨＲＶ随着年龄的增加而下降，尤以迷走神经活性下降为显著。频域指标中各频段的相对面积较绝对面积能更敏感地反映交感、迷走神经张力的消长。     

血清HA、LN水平的测定对陈旧性心梗心肌纤维化的诊断意义

目的 作者应用放射免疫法,检测了57例陈旧心梗病人血清HA、LN水平,并以66例正常人作对照。结果陈旧心梗组血清HA、LN水平明显高于正常值,与对照组间有显著性差异(P<0.001),且增高程度与梗塞面积呈正比。结论血清HA、LN高水平是对陈旧心梗心肌纤维化诊断有价值的指标。     

儿童特应性皮炎312例临床分析

特应性皮炎（AD）是一种慢性、难治性皮肤疾病。因反复发作、剧烈瘙痒而影响患儿的日常生活。笔者对312例儿童AD的临床特征进行分析,现将结果报告如下。     

肝癌射频消融术后并发胸腔脓肿1例

1 临床资料 患者,男,45岁.因肝癌切除术后72d,右上腹持续隐痛不适2d,于2005年7月1日入院.患者2005年4月19日因原发性肝癌行右肝Ⅵ、Ⅶ段不规则切除;病理报告:肝细胞性肝癌.