支气管哮喘药物治疗近况的研究评价

目的通过对支气管哮喘药物治疗近况的比较分析,以促进临床合理用药。方法自2009年6月至2010年6月从医院科室共获得143例支气管哮喘的病案资料,对资料中使用的药物进行分类总结对比。结果对比发现抗生素、糖气质激素、β-受体激动剂的支气管哮喘药物在临床上的应用所占比例较大;而白三烯类、茶碱类以及抗组胺药物在临床上的应用所占比例较小。结论加强在临床上治疗支气管哮喘的合理用药管理,尤其注意儿童在用药上加以区别对待。     

早产重症RhD阴性新生儿溶血病换血1例

产妇，孕6产2。人流4次，1984年顺产1男婴，现健康存活。2001年孕35w早产1男婴，生后2d黄疸，因患脑瘫后遗症病故。2003年5月孕28w产前检查新生儿溶血病，结果为Rh(-)，抗-D效价128，孕33w^ 3测抗-D效价256，B超检查未见胎儿有明显水肿或贫血表现，于33w^ 6行剖官产娩出1男婴。患儿脐带水肿、皮肤苍白、四肢及口周发绀，呈贫血早产儿外貌，体重1700g，体温36．3℃，呼吸63次／min，     

品管圈活动在降低药占比管理工作中的应用

目的：通过品管圈方法加强对全院药占比的管理,提高合理用药水平,保障患者用药安全。方法：按照品管圈活动步骤,查找当前药占比偏高的原因,制定相应对策加以实施。结果：通过开展品管圈活动,全院药占比（不含中药饮片）由活动前的31.85%下降到活动后的30.40%。结论：品管圈活动有助于降低药占比,利于质量的持续改进。     

华法林治疗房颤的观察和护理

目的 探讨华法林治疗心房颤动的护理措施.方法 对我院近年来收治的158 例心房颤动患者临床资料及护理措施回顾分析.结果 均行口服华法林抗凝治疗,经治疗及精心护理,均病情好转出院.结论 严密观察出血的不良反应,定期复查凝血酶原,随时调整剂量,通过华法林抗凝治疗的精心护理,提高了治疗效果,起到促进患者康复的重要作用.     

Notch基因细胞内区在子宫颈癌组织中的表达及DAPT对子宫颈癌细胞的影响

Objective To study the expression and clinical significance of Notch intracellular domain (NICD) in cervical cancer and the effects of N-[N-(3,5-difluorophenyl)acetyl-L-alanyl]-S-phenyl glycine t-butyl ester (DAPT), a γ-secretase inhibitor on the proliferation and apoptosis of cervical cancer cell lines. Methods Western blot was used to detect the expression of NICD in the tissues of 40 cervical cancers and 21 normal cervix and its relationship with clinical features of cervical cancer was also analyzed. Proliferation of SiHa and HeLa cervical cells was determined by methyl thiazolyl tetrazolium (MTT) assay, cell cycles and apoptosis and index of proliferation were detected by flow cytometry method. The expression of NICD in SiHa and HeLa cells incubated with DAPT was detected by western blot. Results The expression level of NICD in cervical cancers was significantly higher than that of normal cervical tissues (1.237±0.353 vs 0.938±0.105, P<0.05). The NICD expression was higher in cervical cancers with high grade,lymph node involvement and parametrial invasion than that with low-middle grade (1.496±0.540 vs 1.150±0.216), without lymph node involvement (1.419±0.532 vs 1.159±0.210) and no parametrial invasion (1.718±0.710 vs 1.183±0.258), respectively (all P<0.05). The expression of NICD in cervical adenocarcinoma was higher than that of squamous cell cancer (1.463±0.395 vs 1.162±0.187, P<0.05). After SiHa and HeLa cells were incubated with DAPT, NICD expression was significantly lower than that in control (P<0.05). The effects of DAPT inhibited the proliferation and prompted the apoptosis of SiHa and HeLa cells was depended on its concentrations and times. Conclusions NICD may play a key role in the occurrence and progress of cervical cancer. The mechanism of DAPT inhibited the proliferation and prompted the apoptosis of SiHa and HeLa cells may be due to decreased the formation of NICD.     

子宫广泛性切除加阴道全切除术中行子宫浆膜代阴道成形术治疗阴道黑色素瘤二例分析

阴道黑色素瘤是较罕见的妇科恶性肿瘤,病死率极高,目前尚无理想的治疗方案.清华大学玉泉医院分别于2005年和2007年对两例阴道黑色素瘤患者在子宫广泛性切除+阴道全切除术中行子宫浆膜代阴道成形术治疗.术后患者均恢复正常性生活,目前分别存活20和39个月.